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anti-AS160 antibody :: Rabbit anti-Human, mouse AS160 (Phospho-Thr642) Antibody

Scan QR to view Datasheet Catalog #    MBS002191 anti-AS160 antibody
Unit / Price
0.05 mL  /  $270 +1 FREE 8GB USB
0.1 mL  /  $445 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AS160 (Phospho-Thr642), Antibody

★Popular Item★
 Also Known As   

Rabbit AS160 (Phospho-Thr642) Antibody

 Product Gene Name   

anti-AS160 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 13; NC_000013.10 (75858809..76056250, complement). Location: 13q22.2
 OMIM    612465
 3D Structure    ModBase 3D Structure for O60343
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 Host    Rabbit
 Species Reactivity    Human, mouse
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 Specificity    Phospho-TBC1D4 (T642) Polyclonal Antibody dectects endogenous levels of TBC1D4 protein only when phosphorylated at T642.
 Purity/Purification    The antibody was affinity-purified from rabbit anti-serum by affinity-chromatography using epitope-specific immunogen.
 Form/Format    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% soidum azide.
 Concentration    1 mg/ml (lot specific)
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 Immunogen    Synthesized peptide derived from human TBC1D4 around the phoshorylation site of T642.
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 Preparation and Storage    20 Degree C/ 1 year
 Other Notes    Small volumes of anti-AS160 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-AS160 antibody   

Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA (EIA)

 Application Notes for anti-AS160 antibody    Immunohistochemistry: 1/100-1/300
Immunofluorescence: 1/200-1/1000.
ELISA: 1/5000. Not yet tested in other applications.
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NCBI/Uniprot data below describe general gene information for AS160. It may not necessarily be applicable to this product.
 NCBI GI #    114688046
 NCBI GeneID    9882
 NCBI Accession #    NP_055647.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_014832.2 [Other Products]
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 UniProt Primary Accession #    O60343 [Other Products]
 UniProt Secondary Accession #    Q5W0B9; Q68D14; A7E2X8; B4DU25; B4E235; B6ETN8; B6ETN9 [Other Products]
 UniProt Related Accession #    O60343 [Other Products]
 Molecular Weight    146,563 Da
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 NCBI Official Full Name    TBC1 domain family member 4
 NCBI Official Synonym Full Names    TBC1 domain family, member 4
 NCBI Official Symbol    TBC1D4 [Similar Products]
 NCBI Official Synonym Symbols   
AS160
[Similar Products]
 NCBI Protein Information    TBC1 domain family member 4; akt substrate of 160 kDa; Acrg embryonic lethality minimal region ortholog; TBC (Tre-2, BUB2, CDC16) domain-containing protein
 UniProt Protein Name    TBC1 domain family member 4
 UniProt Synonym Protein Names   
Akt substrate of 160 kDa
 UniProt Gene Name    TBC1D4 [Similar Products]
 UniProt Synonym Gene Names    AS160; KIAA0603; AS160 [Similar Products]
 UniProt Entry Name    TBCD4_HUMAN
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 NCBI Summary for AS160    This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]
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 UniProt Comments for AS160    AS160: a widely-expressed Rab GTPase-activating protein which apparently plays a role in mediating insulin action. Phosphorylated by the Ser/Thr protein kinase Akt in response to insulin. Is redistributed from low-density microsomes to the cytosol upon insulin treatment. Insulin stimulation of GLUT4 exocytosis is dependent on AS160. The translocation of GLUT4 from intracellular storage sites to the cell surface functions to stimulate insulin uptake by adipose and muscle tissue. Its expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects; its phosphorylation is impaired in skeletal muscle of type 2 diabetics. The protein is expressed in pancreatic Langerhans islets, including beta cells. Up-regulated in T cells from patients with atopic dermatitis. Two alternatively spliced isoforms have been described. Isoform 2, which promotes GLUT4 translocation at the plasma membrane, is the highest overexpressed in most tissues. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver or adipose tissue.

Protein type: GAPs; GAPs, Rab

Chromosomal Location of Human Ortholog: 13q22.2

Cellular Component: cytoplasmic vesicle membrane; cytoplasm

Biological Process: vesicle-mediated transport; negative regulation of vesicle fusion; cellular response to insulin stimulus

Disease: Diabetes Mellitus, Noninsulin-dependent, 5
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 Research Articles on AS160    1. insulin resistance in muscles from healthy individuals is associated with suppression of site-specific phosphorylation of AS160
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-AS160 antibodyDiseases associated with anti-AS160 antibody
 Products by Pathway  Pathway Diagram
 Class I PI3K Signaling Events Mediated By Akt Pathway antibodies  Class I PI3K Signaling Events Mediated By Akt Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Insulin-mediated Glucose Transport Pathway antibodies  Insulin-mediated Glucose Transport Pathway Diagram
 Membrane Trafficking Pathway antibodies  Membrane Trafficking Pathway Diagram
 Translocation Of GLUT4 To The Plasma Membrane Pathway antibodies  Translocation Of GLUT4 To The Plasma Membrane Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >5 publications with AS160 and Inflammation
 Neoplasms Antibodies  >4 publications with AS160 and Neoplasms
 Liver Diseases Antibodies  >3 publications with AS160 and Liver Diseases
 Necrosis Antibodies  >3 publications with AS160 and Necrosis
 Weight Loss Antibodies  >2 publications with AS160 and Weight Loss
 Hypertrophy Antibodies  >1 publications with AS160 and Hypertrophy
 Fatty Liver Antibodies  >1 publications with AS160 and Fatty Liver
 Breast Neoplasms Antibodies  >1 publications with AS160 and Breast Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with AS160 and Carcinoma, Hepatocellular
 Kidney Diseases Antibodies  >1 publications with AS160 and Kidney Diseases
Organs/Tissues associated with anti-AS160 antibody
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >79 publications with AS160 and Muscle
 Blood Antibodies  >27 publications with AS160 and Blood
 Kidney Antibodies  >8 publications with AS160 and Kidney
 Liver Antibodies  >7 publications with AS160 and Liver
 Connective Tissue Antibodies  >6 publications with AS160 and Connective Tissue
 Heart Antibodies  >3 publications with AS160 and Heart
 Embryonic Tissue Antibodies  >3 publications with AS160 and Embryonic Tissue
 Lung Antibodies  >2 publications with AS160 and Lung
 Ovary Antibodies  >2 publications with AS160 and Ovary
 Uterus Antibodies  >1 publications with AS160 and Uterus
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