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anti-Akt2 antibody :: Akt2 Antibody

Scan QR to view Datasheet Catalog #    MBS857574
Unit / Price
0.1 mg  /  $215 +1 FREE 8GB USB
0.05 mg  /  $225 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Akt2, Antibody

 Also Known As   

Akt2 (Phospho-Ser474) Antibody

 Product Gene Name   

anti-Akt2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    125853
 3D Structure    ModBase 3D Structure for P31751
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 Species Reactivity    Human, Mouse, Rat
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 Specificity    Akt2 (phospho-Ser474) antibody detects endogenous levels of Akt2 only when phosphorylated at serine 474.
 Purity/Purification    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
 Form/Format    In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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 Immunogen    The antiserum was produced against synthesized phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-SP-Y-S).
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 Preparation and Storage    Store at-20 degree C for 1 year
 Other Notes    Small volumes of anti-Akt2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-Akt2 antibody    Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
 Applications Tested/Suitable for anti-Akt2 antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-Akt2 antibody    WB: 1:500~1:1000
IHC: 1:50~1:100
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 Immunohistochemistry (IHC) of anti-Akt2 antibody    P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2 (phospho-Ser474) antibody.
anti-Akt2 antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-Akt2 antibody    Western blot analysis using Akt2 (Ab-474) antibody and Akt2 (phospho-Ser474) antibody.
anti-Akt2 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for Akt2. It may not necessarily be applicable to this product.
 NCBI GI #    1059842869
 NCBI GeneID    208
 NCBI Accession #    NP_001317440.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001330511.1 [Other Products]
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 UniProt Primary Accession #    P31751 [Other Products]
 UniProt Secondary Accession #    Q05BV0; Q0VAN0; Q0VAN1; Q68GC0; B2RBD8 [Other Products]
 UniProt Related Accession #    P31751 [Other Products]
 Molecular Weight    51,083 Da
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 NCBI Official Full Name    RAC-beta serine/threonine-protein kinase isoform 4
 NCBI Official Synonym Full Names    AKT serine/threonine kinase 2
 NCBI Official Symbol    AKT2 [Similar Products]
 NCBI Official Synonym Symbols   
PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
[Similar Products]
 NCBI Protein Information    RAC-beta serine/threonine-protein kinase
 UniProt Protein Name    RAC-beta serine/threonine-protein kinase
 UniProt Synonym Protein Names   
Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta
 Protein Family    Potassium channel
 UniProt Gene Name    AKT2 [Similar Products]
 UniProt Synonym Gene Names    PKB beta [Similar Products]
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 NCBI Summary for Akt2    This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq, Jul 2008]
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 UniProt Comments for Akt2    AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI3P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development.One of the few specific substrates of AKT2 identified recently is PITX2. Phosphorylation of PITX2 impairs its association with the CCND1 mRNA-stabilizing complex thus shortening the half-life of CCND1. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. Phosphorylates C2CD5 on 'Ser-197' during insulin-stimulated adipocytes. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis. Phosphorylates CLK2 on 'Thr-343'.CautionIn light of strong homologies in the primary amino acid sequence, the 3 AKT kinases were long surmised to play redundant and overlapping roles. More recent studies has brought into question the redundancy within AKT kinase isoforms and instead pointed to isoform specific functions in different cellular events and diseases. AKT1 is more specifically involved in cellular survival pathways, by inhibiting apoptotic processes; whereas AKT2 is more specific for the insulin receptor signaling pathway. Moreover, while AKT1 and AKT2 are often implicated in many aspects of cellular transformation, the 2 isoforms act in a complementary opposing manner. The role of AKT3 is less clear, though it appears to be predominantly expressed in brain.
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Product References and Citations for anti-Akt2 antibody

   Sun M, et al. (2001) Cancer Res; 61(16): 5985-91. Yuan ZQ, et al. (2000) Oncogene; 19(19): 2324-30. Meier R, et al. (1997) J Biol Chem; 272(48): 30491-7.
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 Research Articles on Akt2    1. miR2965p/AKT2 axis serves important roles in Hepatocellular carcinoma carcinogenesis and progression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-Akt2 antibodyPathways associated with anti-Akt2 antibody
 Reference Product  PubMed Publications
 MTOR antibody  >121 publications with Akt2 and MTOR
 FOXO1 antibody  >42 publications with Akt2 and FOXO1
 TBC1D4 antibody  >13 publications with Akt2 and TBC1D4
 FOXO3 antibody  >13 publications with Akt2 and FOXO3
 PHLPP1 antibody  >8 publications with Akt2 and PHLPP1
 TSC2 antibody  >7 publications with Akt2 and TSC2
 FOXO4 antibody  >3 publications with Akt2 and FOXO4
 INS antibody  >3 publications with Akt2 and INS
 TSC1 antibody  >3 publications with Akt2 and TSC1
 TCL1B antibody  >1 publications with Akt2 and TCL1B
 Products by Pathway  Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AKT Phosphorylates Targets In The Cytosol Pathway antibodies  AKT Phosphorylates Targets In The Cytosol Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 AKT-mediated Inactivation Of FOXO1A Pathway antibodies  AKT-mediated Inactivation Of FOXO1A Pathway Diagram
 AMP-activated Protein Kinase (AMPK) Signaling Pathway antibodies  AMP-activated Protein Kinase (AMPK) Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of AKT2 Pathway antibodies  Activation Of AKT2 Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
Diseases associated with anti-Akt2 antibodyOrgans/Tissues associated with anti-Akt2 antibody
 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >162 publications with Akt2 and Insulin Resistance
 Adenocarcinoma Antibodies  >92 publications with Akt2 and Adenocarcinoma
 Breast Neoplasms Antibodies  >92 publications with Akt2 and Breast Neoplasms
 Disease Models, Animal Antibodies  >74 publications with Akt2 and Disease Models, Animal
 Ovarian Neoplasms Antibodies  >72 publications with Akt2 and Ovarian Neoplasms
 Lung Neoplasms Antibodies  >56 publications with Akt2 and Lung Neoplasms
 Liver Diseases Antibodies  >55 publications with Akt2 and Liver Diseases
 Cardiovascular Diseases Antibodies  >48 publications with Akt2 and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >43 publications with Akt2 and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >42 publications with Akt2 and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >225 publications with Akt2 and Muscle
 Blood Antibodies  >167 publications with Akt2 and Blood
 Liver Antibodies  >127 publications with Akt2 and Liver
 Lung Antibodies  >84 publications with Akt2 and Lung
 Vascular Antibodies  >75 publications with Akt2 and Vascular
 Adipose Tissue Antibodies  >63 publications with Akt2 and Adipose Tissue
 Brain Antibodies  >59 publications with Akt2 and Brain
 Heart Antibodies  >55 publications with Akt2 and Heart
 Bone Antibodies  >47 publications with Akt2 and Bone
 Connective Tissue Antibodies  >42 publications with Akt2 and Connective Tissue
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