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anti-AR antibody :: Rabbit Androgen Receptor (AR) Antibody

Scan QR to view Datasheet Catalog #    MBS462010
Testing Data
Unit / Price
0.1 mg  /  $245 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Androgen Receptor (AR), Antibody

 Also Known As   

Rabbit anti AR antibody

 Product Synonym Names    Rabbit anti-AR (NT); Androgen Receptor; Dihydrotestosterone Receptor
 Product Gene Name   

anti-AR antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: X; NC_000023.10 (66763874..66944119). Location: Xq12
 OMIM    300068
 3D Structure    ModBase 3D Structure for P10275
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 Host    Rabbit
 Species Reactivity    Human, Rat, Mouse, Canine
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 Specificity    This antibody recognizes ~110 kDa of human AR protein. It also reacts with mouse and rat. The other species are not tested.
 Purity/Purification    The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
 Form/Format    This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
 Concentration    Size: 100 ug/200 ul (lot specific)
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 Antigen Preparation    A synthetic peptide corresponding to N-term of human AR. This sequence is identical among human, rat, mouse, dog.
 Positive Control    LNCaP prostate Ca Cell
 Cellular Location    Nuclear
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 Preparation and Storage    The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
 Other Notes    Small volumes of anti-AR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-AR antibody

   The androgen receptor (AR), a member of nuclear receptor family, is essential for the growth of prostate cancer cells. The phosphorylation of AR is induced by growth factors and elevated in hormone-refractory prostate tumors. It's been reported the signal pathway of the PI3K/AKT, Src to be responsible for phosphorylation of AR at Ser213/210 and Ser81. The abnormal activation of AR is observed in many diseases, such as prostate cancer.
 Applications Tested/Suitable for anti-AR antibody   

ELISA, Western Blot, Immunohistochemistry

 Application Notes for anti-AR antibody    Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
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 Testing Data of anti-AR antibody    anti-AR antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for AR. It may not necessarily be applicable to this product.
 NCBI GI #    178882
 NCBI GeneID    367
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 UniProt Primary Accession #    P10275 [Other Products]
 UniProt Secondary Accession #    Q9UD95; A2RUN2; B1AKD7 [Other Products]
 UniProt Related Accession #    P10275; Q13771; Q9BZG5; Q9BZG6; Q9BZG7; Q9NUA2; Q9UN21 [Other Products]
 Molecular Weight    110 kDa
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 NCBI Official Full Name    androgen receptor
 NCBI Official Synonym Full Names    androgen receptor
 NCBI Official Symbol    AR [Similar Products]
 NCBI Official Synonym Symbols   
KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
[Similar Products]
 NCBI Protein Information    androgen receptor; OTTHUMP00000023450; OTTHUMP00000217211; dihydrotestosterone receptor; androgen nuclear receptor variant 2; nuclear receptor subfamily 3 group C member 4
 UniProt Protein Name    Androgen receptor
 UniProt Synonym Protein Names   
Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4
 Protein Family    Allatostatin
 UniProt Gene Name    AR [Similar Products]
 UniProt Synonym Gene Names    DHTR; NR3C4 [Similar Products]
 UniProt Entry Name    ANDR_HUMAN
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 NCBI Summary for AR    The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq]
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 UniProt Comments for AR    Function: Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3. Ref.33 Ref.44 Ref.51 Ref.56 Ref.58

Enzyme regulation: AIM-100 (4-amino-5,6-biaryl-furo[2,3-d]pyrimidine) suppresses TNK2-mediated phosphorylation at Tyr-267. Inhibits the binding of the Tyr-267 phosphorylated form to androgen-responsive enhancers (AREs) and its transcriptional activity. Ref.47

Subunit structure: Binds DNA as a homodimer. Part of a ternary complex containing AR, EFCAB6/DJBP and PARK7. Interacts with HIPK3 and NR0B2 in the presence of androgen. The ligand binding domain interacts with MYST2/HBO1 in the presence of dihydrotestosterone. Interacts with EFCAB6/DJBP, PELP1, PQBP1, RANBP9, RBAK, SPDEF, SRA1, TGFB1I1, ZNF318 and RREB1. Interacts with ZMIZ1/ZIMP10 and ZMIZ2/ZMIP7 which both enhance its transactivation activity. Interacts with SLC30A9 and RAD54L2/ARIP4

By similarity. Interacts via the ligand-binding domain with LXXLL and FXXLF motifs from NCOA1, NCOA2, NCOA3, NCOA4 and MAGEA11. The AR N-terminal poly-Gln region binds Ran resulting in enhancement of AR-mediated transactivation. Ran-binding decreases as the poly-Gln length increases. Interacts with HIP1 (via coiled coil domain). Interacts (via ligand-binding domain) with TRIM68. Interacts with TNK2. Interacts with USP26. Interacts with RNF6. Interacts (regulated by RNF6 probably through polyubiquitination) with RNF14; regulates AR transcriptional activity. Interacts with PRMT2 and TRIM24. Interacts with GNB2L1/RACK1. Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.27 Ref.29 Ref.30 Ref.31 Ref.32 Ref.33 Ref.34 Ref.35 Ref.36 Ref.39 Ref.40 Ref.41 Ref.42 Ref.43 Ref.44 Ref.46 Ref.50 Ref.51 Ref.56 Ref.58

Subcellular location: Nucleus. Cytoplasm. Note: Predominantly cytoplasmic in unliganded form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unliganded form in the presence of GNB2L1. Ref.7 Ref.32 Ref.39 Ref.44

Tissue specificity: Isoform 2 is mainly expressed in heart and skeletal muscle. Ref.7

Domain: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain. Ref.50 Ref.56

Post-translational modification: Sumoylated on Lys-386 (major) and Lys-520. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity. Ref.44 Ref.46Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-534 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Ref.37 Ref.41 Ref.45 Ref.47

Polymorphism: The poly-Gln region of AR is highly polymorphic and the number of Gln varies in the population (from 17 to 26). A smaller size of the poly-Gln region may be associated with the development of prostate cancer.The poly-Gly region of AR is polymorphic and ranges from 24 to 31 Gly. A poly-Gly region shorter or equal to 23 may be associated with the development of androgenetic alopecia.

Involvement in disease: Defects in AR are the cause of androgen insensitivity syndrome (AIS) [

MIM:300068]; previously known as testicular feminization syndrome (TFM). AIS is an X-linked recessive form of pseudohermaphroditism due end-organ resistance to androgen. Affected males have female external genitalia, female breast development, blind vagina, absent uterus and female adnexa, and abdominal or inguinal testes, despite a normal 46,XY karyotype. Ref.4 Ref.16 Ref.18 Ref.52 Ref.66 Ref.68 Ref.71 Ref.73 Ref.74 Ref.75 Ref.76 Ref.77 Ref.78 Ref.80 Ref.81 Ref.85 Ref.86 Ref.88 Ref.90 Ref.92 Ref.94 Ref.96 Ref.105 Ref.106 Ref.107 Ref.109 Ref.110 Ref.111 Ref.112 Ref.113 Ref.114 Ref.116 Ref.117 Ref.119 Ref.120 Ref.121 Ref.127 Ref.129 Ref.130 Ref.133 Ref.134 Ref.136 Ref.137 Ref.138 Ref.139 Ref.142 Ref.145 Ref.146 Ref.147 Ref.150 Ref.152 Ref.153 Ref.154 Ref.155 Ref.156 Ref.157 Ref.158 Ref.159 Ref.160 Ref.162 Ref.165 Ref.166 Ref.171 Ref.172 Ref.173 Ref.174 Ref.178 Ref.181 Ref.182 Ref.185 Ref.186 Ref.188 Ref.191Defects in AR are the cause of spinal and bulbar muscular atrophy X-linked type 1 (SMAX1) [

MIM:313200]; also known as Kennedy disease. SMAX1 is an X-linked recessive form of spinal muscular atrophy. Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX1 occurs only in men. Age at onset is usually in the third to fifth decade of life, but earlier involvement has been reported. It is characterized by slowly progressive limb and bulbar muscle weakness with fasciculations, muscle atrophy, and gynecomastia. The disorder is clinically similar to classic forms of autosomal spinal muscular atrophy. Note=Caused by trinucleotide CAG repeat expansion. In SMAX1 patients the number of Gln ranges from 38 to 62. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. Ref.190Note=Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.Defects in AR are the cause of androgen insensitivity syndrome partial (PAIS) [

MIM:312300]; also known as Reifenstein syndrome. PAIS is characterized by hypospadias, hypogonadism, gynecomastia, genital ambiguity, normal XY karyotype, and a pedigree pattern consistent with X-linked recessive inheritance. Some patients present azoospermia or severe oligospermia without other clinical manifestations.

Miscellaneous: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone-receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites.Transcriptional activity is enhanced by binding to RANBP9.The level of tyrosine phosphorylation may serve as a diagnostic tool to predict patient outcome in response to hormone-ablation therapy. Inhibition of tyrosine phosphorylation may be an effective intervention target for hormone-refractory prostate cancer.

Sequence similarities: Belongs to the nuclear hormone receptor family. NR3 subfamily.Contains 1 nuclear receptor DNA-binding domain.
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Product References and Citations for anti-AR antibody

   Yasumasa Ikeda, et al (2005), Androgen Receptor Gene Knockout Male Mice Exhibit Impaired Cardiac Growth and Exacerbation of Angiotensin II-induced Cardiac Fibrosis. J. Biol. Chem., Vol. 280, Issue 33, 29661-29666.
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 Research Articles on AR    1. Report reciprocal feedback regulation of PI3K and androgen receptor signaling in PTEN-deficient prostate cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-AR antibodyPathways associated with anti-AR antibody
 Reference Product  PubMed Publications
 JUN antibody  >494 publications with AR and JUN
 CTNNB1 antibody  >477 publications with AR and CTNNB1
 CASP3 antibody  >366 publications with AR and CASP3
 EGFR antibody  >307 publications with AR and EGFR
 MAPK1 antibody  >256 publications with AR and MAPK1
 CCND1 antibody  >228 publications with AR and CCND1
 PTEN antibody  >150 publications with AR and PTEN
 HIF1A antibody  >114 publications with AR and HIF1A
 AKT1 antibody  >112 publications with AR and AKT1
 AHR antibody  >110 publications with AR and AHR
 Products by Pathway  Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Coregulation Of Androgen Receptor Activity Pathway antibodies  Coregulation Of Androgen Receptor Activity Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 IL-6 Signaling Pathway antibodies  IL-6 Signaling Pathway Diagram
 Nongenotropic Androgen Signaling Pathway antibodies  Nongenotropic Androgen Signaling Pathway Diagram
 Notch-mediated HES/HEY Network Pathway antibodies  Notch-mediated HES/HEY Network Pathway Diagram
 Nuclear Receptor Transcription Pathway antibodies  Nuclear Receptor Transcription Pathway Diagram
Diseases associated with anti-AR antibodyOrgans/Tissues associated with anti-AR antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >8167 publications with AR and Neoplasms
 Autoimmune Diseases Antibodies  >5461 publications with AR and Autoimmune Diseases
 Cardiovascular Diseases Antibodies  >5314 publications with AR and Cardiovascular Diseases
 Inflammation Antibodies  >3456 publications with AR and Inflammation
 Prostatic Neoplasms Antibodies  >2096 publications with AR and Prostatic Neoplasms
 Congenital Abnormalities Antibodies  >2083 publications with AR and Congenital Abnormalities
 Urologic Diseases Antibodies  >1543 publications with AR and Urologic Diseases
 Kidney Diseases Antibodies  >1457 publications with AR and Kidney Diseases
 Hypertension Antibodies  >1069 publications with AR and Hypertension
 Liver Diseases Antibodies  >944 publications with AR and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >9971 publications with AR and Muscle
 Skin Antibodies  >6943 publications with AR and Skin
 Vascular Antibodies  >4272 publications with AR and Vascular
 Brain Antibodies  >3778 publications with AR and Brain
 Prostate Antibodies  >3223 publications with AR and Prostate
 Lung Antibodies  >1784 publications with AR and Lung
 Embryonic Tissue Antibodies  >1759 publications with AR and Embryonic Tissue
 Eye Antibodies  >1129 publications with AR and Eye
 Uterus Antibodies  >500 publications with AR and Uterus
 Mammary Gland Antibodies  >164 publications with AR and Mammary Gland
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