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anti-APAF1 antibody :: Rabbit Apaf-1 (CT) Antibody

Scan QR to view Datasheet Catalog #    MBS194022 anti-APAF1 antibody
Unit / Price
0.1 mg  /  $400 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Apaf-1 (CT) (APAF1), Antibody

 Also Known As   

Anti-Apaf1 (CT) Antibody

 Product Synonym Names    Anti-Apaf-1 (CT); Apaf1 (CT)
 Product Gene Name   

anti-APAF1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 12; NC_000012.11 (99039078..99129211). Location: 12q23
 OMIM    602233
 3D Structure    ModBase 3D Structure for O14727
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 Host    Rabbit
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 Specificity    Anti-Apaf-1 (CT) [Rabbit antibody to peptide corresponding to aa 1158-1177 of human Apaf1. Recognizes human, mouse, and rat Apaf1.]
This antibody recognizes human, mouse, and rat Apaf1 (130 kD).
 Form/Format    100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chroma-tography.
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 Antigen    Peptide corresponding to aa 1158-1177 of human Apaf1. The sequence of the immunogenic peptide differs from that of mouse Apaf1 by one amino acid.
 Stabilizer    None
 Preservatives    0.02% sodium azide
 Dilutions Instructions    Dilute in PBS or medium which is identical to that used in the assay system.
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 Preparation and Storage    This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.
 Other Notes    Small volumes of anti-APAF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-APAF1 antibody

   Apoptosis is related to many diseases and induced by a familyof cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.
 Product Categories/Family for anti-APAF1 antibody    Apoptosis Antibodies
 Applications Tested/Suitable for anti-APAF1 antibody   


 Application Notes for anti-APAF1 antibody    Immunoblotting: use at 1:500-1:2,000 dilution.
Positive control: Human heart tissue lysate.
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NCBI/Uniprot data below describe general gene information for APAF1. It may not necessarily be applicable to this product.
 NCBI GI #    32483363
 NCBI GeneID    317
 NCBI Accession #    NP_863659.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_181869.1 [Other Products]
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 UniProt Primary Accession #    O14727 [Other Products]
 UniProt Secondary Accession #    O43297; Q7Z438; Q9BXZ6; Q9UBZ5; Q9UGN8; Q9UGN9; Q9UGP0; Q9UJ58; Q9UJ59; Q9UJ60; B2RMX8 [Other Products]
 UniProt Related Accession #    O14727 [Other Products]
 Molecular Weight    141,840 Da [Similar Products]
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 NCBI Official Full Name    apoptotic protease-activating factor 1 isoform e
 NCBI Official Synonym Full Names    apoptotic peptidase activating factor 1
 NCBI Official Symbol    APAF1 [Similar Products]
 NCBI Official Synonym Symbols   
CED4; APAF-1; DKFZp781B1145
[Similar Products]
 NCBI Protein Information    apoptotic protease-activating factor 1
 UniProt Protein Name    Apoptotic protease-activating factor 1
 Protein Family    Apoptotic protease-activating factor
 UniProt Gene Name    APAF1 [Similar Products]
 UniProt Synonym Gene Names    KIAA0413 [Similar Products]
 UniProt Entry Name    APAF_HUMAN
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 NCBI Summary for APAF1    This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq]
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 UniProt Comments for APAF1    APAF: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). By E2F and p53/TP53 in apoptotic neurons. Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 12q23

Cellular Component: cytosol; nucleus; apoptosome

Molecular Function: identical protein binding; protein binding; nucleotide binding; caspase activator activity; ADP binding; ATP binding

Biological Process: caspase activation; regulation of apoptosis; nervous system development; neuron apoptosis; positive regulation of apoptosis; apoptosis; forebrain development; neural tube closure; caspase activation via cytochrome c
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 Research Articles on APAF1    1. Expression of Apaf-1 was up-regulated in MCF-7 cells treated with 5-Aza-CdR.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-APAF1 antibodyPathways associated with anti-APAF1 antibody
 Reference Product  PubMed Publications
 CASP9 antibody  >352 publications with APAF1 and CASP9
 CASP3 antibody  >243 publications with APAF1 and CASP3
 Products by Pathway  Pathway Diagram
 Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway antibodies  Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation By HSP70 Pathway antibodies  Apoptosis Modulation By HSP70 Pathway Diagram
Diseases associated with anti-APAF1 antibodyOrgans/Tissues associated with anti-APAF1 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >239 publications with APAF1 and Neoplasms
 Carcinoma Antibodies  >68 publications with APAF1 and Carcinoma
 Leukemia Antibodies  >43 publications with APAF1 and Leukemia
 Melanoma Antibodies  >35 publications with APAF1 and Melanoma
 Skin Diseases Antibodies  >34 publications with APAF1 and Skin Diseases
 Drug Toxicity Antibodies  >34 publications with APAF1 and Drug Toxicity
 Urogenital Neoplasms Antibodies  >33 publications with APAF1 and Urogenital Neoplasms
 Adenocarcinoma Antibodies  >28 publications with APAF1 and Adenocarcinoma
 Neurodegenerative Diseases Antibodies  >26 publications with APAF1 and Neurodegenerative Diseases
 Colorectal Neoplasms Antibodies  >25 publications with APAF1 and Colorectal Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >80 publications with APAF1 and Brain
 Blood Antibodies  >67 publications with APAF1 and Blood
 Muscle Antibodies  >43 publications with APAF1 and Muscle
 Heart Antibodies  >31 publications with APAF1 and Heart
 Embryonic Tissue Antibodies  >26 publications with APAF1 and Embryonic Tissue
 Eye Antibodies  >23 publications with APAF1 and Eye
 Skin Antibodies  >21 publications with APAF1 and Skin
 Thymus Antibodies  >13 publications with APAF1 and Thymus
 Testis Antibodies  >11 publications with APAF1 and Testis
 Bone Marrow Antibodies  >11 publications with APAF1 and Bone Marrow
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