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anti-APOB antibody :: Goat Apolipoprotein B-100 Antibody

Scan QR to view Datasheet Catalog #    MBS390016 anti-APOB antibody
Unit / Price
0.5 mL  /  $290 +1 FREE 8GB USB
1 mL  /  $435 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Apolipoprotein B-100 (APOB), Antibody

★Popular Item★
 Also Known As   

HRP-conjugated Goat Anti-Human Apolipoprotein B-100

 Product Synonym Names    Horseradish Peroxidase (HRP) conjugated Goat Anti-Human Apolipoprotein B-100/48 (Apo B-100/48), Polyclonal Antibody
 Product Gene Name   

anti-APOB antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 2; NC_000002.11 (21224301..21266945, complement). Location: 2p24-p23
 OMIM    107730
 3D Structure    ModBase 3D Structure for P04114
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 Host    Goat
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 Specificity    Specifically binds to human apo B-48/100. Dilution for immunoblot and ELISA range: 200 to 8,000.
 Form/Format    1 ml in Freeze-dried powder.
 Concentration    1 mg / ml (lot specific)
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 Source Note    Polyclonal antibody purified by human apolipoprotein B-100- Sepharose affinity column, and conjugated with HRP.
 Preservative    50 mM PBS, 0.1 M NaCl, pH 7.4 and 0.01% Thimersol.
 Stabilizer    10 mg / ml Bovine Serum Albumin.
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 Preparation and Storage    Freeze-dried product should be stored refrigerated until opened. After opening, restore to 0.5 ml with distilled water. If it is not completely clear after standing for 1-2 Hours at room temperature, centrifuge the product. It is stable for several weeks at 4 degree C as an undiluted liquid. Do not use for more than one day after dilution. For extended storage after reconstitution, we suggest aliquot to avoid repeated freezing and thawing; or the addition of an equal volume of glycerol to make a final glycerol concentration of 50%, followed by storage at -20 degree C. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol
 Other Notes    Small volumes of anti-APOB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-APOB antibody    HRP Antibodies
 Applications Tested/Suitable for anti-APOB antibody   

ELISA (EIA), Immunoblot (IB)

 Application Notes for anti-APOB antibody    Freeze-dried powder. Dilution for Immunoblot & ELISA range: 1,000 to 8,000.
Use: The antibody can be used for detection of human apo B-48/100 in plasma and lipoproteins, immunoassays and immunoblots.
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NCBI/Uniprot data below describe general gene information for APOB. It may not necessarily be applicable to this product.
 NCBI GI #    105990532
 NCBI GeneID    338
 NCBI Accession #    NP_000375.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000384.2 [Other Products]
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 UniProt Primary Accession #    P04114 [Other Products]
 UniProt Secondary Accession #    O00502; P78479; P78480; P78481; Q13779; Q13785; Q13786; Q13787; Q13788; Q4ZG63; Q53QC8 [Other Products]
 UniProt Related Accession #    P04114 [Other Products]
 Molecular Weight    515,605 Da [Similar Products]
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 NCBI Official Full Name    apolipoprotein B-100
 NCBI Official Synonym Full Names    apolipoprotein B
 NCBI Official Symbol    APOB [Similar Products]
 NCBI Official Synonym Symbols   
FLDB; LDLCQ4
[Similar Products]
 NCBI Protein Information    apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; apolipoprotein B48; apolipoprotein B (including Ag(x) antigen)
 UniProt Protein Name    Apolipoprotein B-100
 Protein Family    Apolipoprotein
 UniProt Gene Name    APOB [Similar Products]
 UniProt Synonym Gene Names    Apo B-100; Apo B-48 [Similar Products]
 UniProt Entry Name    APOB_HUMAN
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 NCBI Summary for APOB    This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]
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 UniProt Comments for APOB    Function: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Subunit structure: Interacts with PCSK9. Ref.38

Subcellular location: Cytoplasm. Secreted Ref.38.

Induction: Up-regulated in response to enterovirus 71 (EV71) infection (at protein level). Ref.34

Post-translational modification: Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. Ref.31

Involvement in disease: Familial hypobetalipoproteinemia 1 (FHBL1) [MIM:107730]: A disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.46Familial ligand-defective apolipoprotein B-100 (FDB) [MIM:144010]: Dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.40 Ref.42 Ref.44 Ref.49Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Sequence similarities: Contains 1 vitellogenin domain.

RNA editing: Edited at position 2180.The stop codon (UAA) at position 2180 is created by RNA editing. Apo B-48, derived from the fully edited RNA, is produced only in the intestine and is found in chylomicrons. Apo B-48 is a shortened form of apo B-100 which lacks the LDL-receptor region. The unedited version (apo B-100) is produced by the liver and is found in the VLDL and LDL.

Sequence caution: The sequence AAA51752.1 differs from that shown. Reason: Frameshift at positions 942, 951, 1139, 1165, 1164, 1371 and 1385.
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 Research Articles on APOB    1. We used exome sequencing to discover a novel nonsense mutation in exon 26 of APOB (p.K2240X) responsible for low cholesterol and fatty liver in a large kindred.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-APOB antibodyDiseases associated with anti-APOB antibody
 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 LDL-mediated Lipid Transport Pathway antibodies  LDL-mediated Lipid Transport Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >3258 publications with APOB and Cardiovascular Diseases
 Dyslipidemias Antibodies  >2855 publications with APOB and Dyslipidemias
 Atherosclerosis Antibodies  >2435 publications with APOB and Atherosclerosis
 Hyperlipidemias Antibodies  >2380 publications with APOB and Hyperlipidemias
 Coronary Disease Antibodies  >1794 publications with APOB and Coronary Disease
 Heart Diseases Antibodies  >1549 publications with APOB and Heart Diseases
 Hypercholesterolemia Antibodies  >1421 publications with APOB and Hypercholesterolemia
 HYPERCHOLESTEROLEMIA, AUTOSOMAL DOMINANT, TYPE B Antibodies  >696 publications with APOB and HYPERCHOLESTEROLEMIA, AUTOSOMAL DOMINANT, TYPE B
 Hyperlipoproteinemia Type II Antibodies  >696 publications with APOB and Hyperlipoproteinemia Type II
 Insulin Resistance Antibodies  >647 publications with APOB and Insulin Resistance
Organs/Tissues associated with anti-APOB antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7746 publications with APOB and Blood
 Liver Antibodies  >2142 publications with APOB and Liver
 Heart Antibodies  >1397 publications with APOB and Heart
 Intestine Antibodies  >567 publications with APOB and Intestine
 Kidney Antibodies  >415 publications with APOB and Kidney
 Muscle Antibodies  >302 publications with APOB and Muscle
 Brain Antibodies  >136 publications with APOB and Brain
 Lung Antibodies  >115 publications with APOB and Lung
 Embryonic Tissue Antibodies  >55 publications with APOB and Embryonic Tissue
 Spleen Antibodies  >54 publications with APOB and Spleen
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