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| Product Name
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B-Raf (Phospho-Thr753), Antibody
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| Also Known As
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Rabbit B-Raf (Phospho-Thr753) Antibody
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| Chromosome Location
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Chromosome: 7; NC_000007.13 (140433812..140624564, complement). Location: 7q34
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| OMIM
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114500
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| 3D Structure
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ModBase 3D Structure for P15056
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| Host
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Rabbit
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| Species Reactivity
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Human, mouse, rat
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| Form/Format
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Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
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| Concentration
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1 mg/ml (lot specific)
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| Other Notes
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Small volumes of anti-B-Raf antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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| Applications Tested/Suitable for anti-B-Raf antibody
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ELISA (EIA), Western Blot (WB)
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TOP
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| NCBI/Uniprot data below describe general gene information for B-Raf. It may not necessarily be applicable to this product. |
| NCBI GI #
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33188459
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| NCBI GeneID
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673
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| NCBI Accession #
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NP_004324.2
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_004333.4
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[Other Products]
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| UniProt Primary Accession #
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P15056
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[Other Products]
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| UniProt Secondary Accession #
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Q13878; Q3MIN6; Q9UDP8; Q9Y6T3; A4D1T4; B6HY61; B6HY62; B6HY63; B6HY64; B6HY65; B6HY66
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[Other Products]
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| UniProt Related Accession #
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P15056
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[Other Products]
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| Molecular Weight
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84,437 Da
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TOP
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| NCBI Official Full Name
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serine/threonine-protein kinase B-raf
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| NCBI Official Synonym Full Names
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v-raf murine sarcoma viral oncogene homolog B1
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| NCBI Official Symbol
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BRAF
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[Similar Products]
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| NCBI Official Synonym Symbols
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NS7; BRAF1; RAFB1; B-RAF1
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[Similar Products]
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| NCBI Protein Information
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serine/threonine-protein kinase B-raf; 94 kDa B-raf protein; proto-oncogene B-Raf; murine sarcoma viral (v-raf) oncogene homolog B1; B-Raf proto-oncogene serine/threonine-protein kinase (p94)
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| UniProt Protein Name
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Serine/threonine-protein kinase B-raf
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| UniProt Synonym Protein Names
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Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
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| UniProt Gene Name
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BRAF
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[Similar Products]
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| UniProt Synonym Gene Names
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BRAF1; RAFB1
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[Similar Products]
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| UniProt Entry Name
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BRAF_HUMAN
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| NCBI Summary for B-Raf
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This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
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| UniProt Comments for B-Raf
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BRAF: a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib.
Protein type: Oncoprotein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Kinase, protein; TKL group; RAF family
Chromosomal Location of Human Ortholog: 7q34
Cellular Component: neuron projection; mitochondrion; plasma membrane; nucleus; cytosol
Molecular Function: small GTPase binding; identical protein binding; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; protein heterodimerization activity; mitogen-activated protein kinase kinase binding; calcium ion binding; ATP binding; protein kinase activity
Biological Process: positive T cell selection; response to peptide hormone stimulus; response to cAMP; fibroblast growth factor receptor signaling pathway; CD4-positive, alpha beta T cell differentiation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; protein heterooligomerization; somatic stem cell maintenance; MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; synaptic transmission; organ morphogenesis; myeloid progenitor cell differentiation; small GTPase mediated signal transduction; positive regulation of stress fiber formation; visual learning; negative regulation of neuron apoptosis; negative regulation of apoptosis
Disease: Noonan Syndrome 1; Noonan Syndrome 7; Lung Cancer; Leopard Syndrome 3; Cardiofaciocutaneous Syndrome 1
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TOP
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| Research Articles on B-Raf
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1. The novel melanoma cell lines were compared to metastatic cell lines (HBL, LND1), wild type (wt) for MC1R and BRAF genes.
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TOP
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with anti-B-Raf antibody | Pathways associated with anti-B-Raf antibody |
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| Diseases associated with anti-B-Raf antibody | Organs/Tissues associated with anti-B-Raf antibody |
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Antibody 
