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anti-BCL-2 antibody :: Rabbit BCL-2 Antibody

Scan QR to view Datasheet Catalog #    MBS462019
Testing Data
Unit / Price
0.1 mg  /  $285 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

BCL-2, Antibody

 Also Known As   

Rabbit anti BCL-2

 Product Synonym Names    Rabbit anti--Bcl-2; B-cell lymphoma protein 2; (alpha; beta isoforms); BCL2; BCL-2.
 Product Gene Name   

anti-BCL-2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 13; NC_005112.2 (12730736..12905108, complement). Location: 13p13
 3D Structure    ModBase 3D Structure for P49950
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 Host    Rabbit
 Species Reactivity    Human, Rat, Mouse, Bovine, Canine
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 Specificity    This antibody recognizes Bcl-2 with the phosphorylation site Thr129. It does not cross-react with nonphosphospecific peptide.
 Purity/Purification    The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
 Form/Format    This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
 Concentration    Size: 100 ug/200 ul (lot specific)
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 Antigen Preparation    A synthetic peptide derived from C-terminus of human BCL-2 protein. This sequence is identical in human, rat, mouse bovine and dog.
 Positive Control    Raji cells
 Cellular Location    Cytoplasmic
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 Preparation and Storage    The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
 Other Notes    Small volumes of anti-BCL-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-BCL-2 antibody

   The Bcl-2 gene codes for a 25 kDa protein which has been implicated in the control of cell death by apoptosis, in normal tissues, expression of bcl-2 is restricted to cells and tissues that are characterized by apoptotic cell turnover. Bcl-2 protein exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release, thus it is also considered as an inner mitochondrial membrane protein which can be expressed in lymphoma and breast carcinoma cells. This protein modulates mitochondrial calcium homeostasis and proton flux. Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74, Ser87 and Thr127 which may be the targets of the ASK1/MKK7/JNK1 pathway, and that phosphorylation of Bcl-2 may be a marker for mitotic events. Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes. Phosphorylation of Bcl-2 at Ser70 by IL-3/JNK may be required for its enhanced antiapoptotic functions.
 Applications Tested/Suitable for anti-BCL-2 antibody   

ELISA, Western Blot, Immunohistochemistry

 Application Notes for anti-BCL-2 antibody    Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
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 Testing Data of anti-BCL-2 antibody    anti-BCL-2 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for BCL-2. It may not necessarily be applicable to this product.
 NCBI GI #    1004379
 NCBI GeneID    24224
 NCBI Accession #    AAA77687.1 [Other Products]
 NCBI GenBank Nucleotide #    U34964.1 [Other Products]
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 UniProt Primary Accession #    P49950 [Other Products]
 UniProt Secondary Accession #    Q62837; Q64032 [Other Products]
 UniProt Related Accession #    P49950; Q7TSN8; Q9R1B3 [Other Products]
 Molecular Weight    25-26 kDa
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 NCBI Official Full Name    BCL-2
 NCBI Official Synonym Full Names    B-cell CLL/lymphoma 2
 NCBI Official Symbol    Bcl2 [Similar Products]
 NCBI Official Synonym Symbols   
Bcl-2
[Similar Products]
 NCBI Protein Information    apoptosis regulator Bcl-2; Bcl2-like protein; B-cell leukemia/lymphoma 2; B cell lymphoma 2 associated oncogene
 UniProt Protein Name    Apoptosis regulator Bcl-2
 Protein Family    Bcl-2-like protein
 UniProt Gene Name    Bcl2 [Similar Products]
 UniProt Synonym Gene Names    Bcl-2 [Similar Products]
 UniProt Entry Name    BCL2_RAT
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 NCBI Summary for BCL-2    an anti-apoptotic protein; involved in inhibiting cell death in many different cell types [RGD]
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 UniProt Comments for BCL-2    Function: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Subunit structure: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity. Also interacts with APAF1, BBC3, BCL2L1, BNIPL, EI24, FKBP8, MRPL41, RAF-1, TP53BP2. Interacts with BAG1 in an ATP-dependent manner

Subcellular location: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

Tissue specificity: Expressed in a variety of tissues, with highest levels in reproductive tissues. In the adult brain, expression is localized in mitral cells of the olfactory bulb, granule and pyramidal neurons of hippocampus, pontine nuclei, cerebellar granule neurons, and in ependymal cells. In prenatal brain, expression is higher and localized in the neuroepithelium and in the cortical plate.

Domain: The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1

Post-translational modification: Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A)

By similarity.Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity

By similarity.Monoubiquitinated by PARK2, leading to increase its stability

Sequence similarities: Belongs to the Bcl-2 family.
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Product References and Citations for anti-BCL-2 antibody

   Camlica H., et al. Statistical interpretation of CA125 and Bcl-2 in serum of patients with late stage ovarian cancer. Am J Clin Oncol, 2008 Dec, 31 (6): 585-8.
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 Research Articles on BCL-2    1. These results indicated that the Bcl-2 and Bax played a pivotal role in the gastric mucosal I-R injury and repair by activation of ERK1/2.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-BCL-2 antibodyPathways associated with anti-BCL-2 antibody
 Reference Product  PubMed Publications
 Bax antibody  >731 publications with BCL-2 and Bax
 Bcl2l11 antibody  >5 publications with BCL-2 and Bcl2l11
 Products by Pathway  Pathway Diagram
 ATM Pathway antibodies  ATM Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
Diseases associated with anti-BCL-2 antibodyOrgans/Tissues associated with anti-BCL-2 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1723 publications with BCL-2 and Neoplasms
 Lymphoma Antibodies  >752 publications with BCL-2 and Lymphoma
 Lymphoma, B-Cell Antibodies  >532 publications with BCL-2 and Lymphoma, B-Cell
 Leukemia Antibodies  >437 publications with BCL-2 and Leukemia
 Carcinoma Antibodies  >403 publications with BCL-2 and Carcinoma
 Lymphoma, Follicular Antibodies  >278 publications with BCL-2 and Lymphoma, Follicular
 Breast Neoplasms Antibodies  >213 publications with BCL-2 and Breast Neoplasms
 Adenocarcinoma Antibodies  >155 publications with BCL-2 and Adenocarcinoma
 Leukemia, Myeloid Antibodies  >154 publications with BCL-2 and Leukemia, Myeloid
 Neoplasms, Experimental Antibodies  >132 publications with BCL-2 and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >180 publications with BCL-2 and Brain
 Spleen Antibodies  >44 publications with BCL-2 and Spleen
 Artery Antibodies  >41 publications with BCL-2 and Artery
 Pituitary Gland Antibodies  >1 publications with BCL-2 and Pituitary Gland
 Uncharacterized Tissue Antibodies  >1 publications with BCL-2 and Uncharacterized Tissue
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