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anti-BRAF antibody :: Rabbit BRAF-S445 Antibody

Scan QR to view Datasheet Catalog #    MBS125740
Western Blot (WB)
Unit / Price
0.05 mL  /  $265 +1 FREE 8GB USB
0.1 mL  /  $380 +1 FREE 8GB USB
0.2 mL  /  $555 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

BRAF-S445, Antibody

★Popular Item★
 Also Known As   

Phospho-BRAF-S445

 Product Gene Name   

anti-BRAF antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    phenotype 613707
 3D Structure    ModBase 3D Structure for P15056
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 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Purity/Purification    Affinity purification
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 Swiss Prot    P15056
 Species    Human
 Route    Synthetic Peptide
 Immunogen    A phospho specific peptide corresponding to residues surrounding S445 of human BRAF.
 Buffer    PBS with 0.02% sodium azide, 50% glyverol, pH7.3
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 Preparation and Storage    Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze/thaw cycles.
 Other Notes    Small volumes of anti-BRAF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-BRAF antibody    Phosphorylation
 Applications Tested/Suitable for anti-BRAF antibody   

Western Blot (WB)

 Application Notes for anti-BRAF antibody    WB: 1:500 - 1:2000
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 Western Blot (WB) of anti-BRAF antibody    Western blot analysis of extracts of Raji cells, using Phospho-BRAF-S445 antibody at 1:2000 dilution. Raji cells were treated by PMA/TPA (200nM) for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 10s.
anti-BRAF antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for BRAF. It may not necessarily be applicable to this product.
 NCBI GI #    50403720
 NCBI GeneID    673
 NCBI Accession #    P15056.4 [Other Products]
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 UniProt Primary Accession #    P15056 [Other Products]
 UniProt Secondary Accession #    Q13878; Q3MIN6; Q9UDP8; Q9Y6T3; A4D1T4; B6HY61; B6HY62; B6HY63; B6HY64; B6HY65; B6HY66 [Other Products]
 UniProt Related Accession #    P15056 [Other Products]
 Molecular Weight    84kDa
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 NCBI Official Full Name    Serine/threonine-protein kinase B-raf
 NCBI Official Synonym Full Names    B-Raf proto-oncogene, serine/threonine kinase
 NCBI Official Symbol    BRAF [Similar Products]
 NCBI Official Synonym Symbols   
NS7; BRAF1; RAFB1; B-RAF1
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase B-raf; 94 kDa B-raf protein; proto-oncogene B-Raf; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1; murine sarcoma viral (v-raf) oncogene homolog B1; B-Raf proto-oncogene serine/threonine-protein kinase (p94)
 UniProt Protein Name    Serine/threonine-protein kinase B-raf
 UniProt Synonym Protein Names   
Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
 Protein Family    Serine/threonine-protein kinase
 UniProt Gene Name    BRAF [Similar Products]
 UniProt Synonym Gene Names    BRAF1; RAFB1 [Similar Products]
 UniProt Entry Name    BRAF_HUMAN
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 NCBI Summary for BRAF    This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for BRAF    BRAF: a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Oncoprotein; EC 2.7.11.1; TKL group; RAF family

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: neuron projection; mitochondrion; plasma membrane; cytosol; nucleus

Molecular Function: small GTPase binding; protein serine/threonine kinase activity; identical protein binding; protein binding; MAP kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; protein heterodimerization activity; calcium ion binding; ATP binding; protein kinase activity

Biological Process: positive T cell selection; response to peptide hormone stimulus; fibroblast growth factor receptor signaling pathway; response to cAMP; CD4-positive, alpha beta T cell differentiation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; somatic stem cell maintenance; protein heterooligomerization; MAPKKK cascade; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; regulation of cell proliferation; synaptic transmission; organ morphogenesis; myeloid progenitor cell differentiation; small GTPase mediated signal transduction; positive regulation of stress fiber formation; visual learning; negative regulation of neuron apoptosis; negative regulation of apoptosis

Disease: Noonan Syndrome 1; Noonan Syndrome 7; Lung Cancer; Cardiofaciocutaneous Syndrome 1; Leopard Syndrome 3
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 Research Articles on BRAF    1. The mutations of EGFR and BRAF genes were not found in HER2-mutated patients with brain metastasis from non-small cell lung cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-BRAF antibodyPathways associated with anti-BRAF antibody
 Reference Product  PubMed Publications
 HRAS antibody  >170 publications with BRAF and HRAS
 MAP2K1 antibody  >118 publications with BRAF and MAP2K1
 MAP2K2 antibody  >71 publications with BRAF and MAP2K2
 RAF1 antibody  >61 publications with BRAF and RAF1
 YWHAE antibody  >1 publications with BRAF and YWHAE
 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
Diseases associated with anti-BRAF antibodyOrgans/Tissues associated with anti-BRAF antibody
 Disease Name  Pubmed Publications
 Melanoma Antibodies  >2066 publications with BRAF and Melanoma
 Neuroendocrine Tumors Antibodies  >1430 publications with BRAF and Neuroendocrine Tumors
 Colorectal Neoplasms Antibodies  >1165 publications with BRAF and Colorectal Neoplasms
 Thyroid Neoplasms Antibodies  >921 publications with BRAF and Thyroid Neoplasms
 Neoplasm Metastasis Antibodies  >523 publications with BRAF and Neoplasm Metastasis
 Lung Neoplasms Antibodies  >308 publications with BRAF and Lung Neoplasms
 Lung Diseases Antibodies  >263 publications with BRAF and Lung Diseases
 Nervous System Diseases Antibodies  >248 publications with BRAF and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >240 publications with BRAF and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >202 publications with BRAF and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Thyroid Antibodies  >1134 publications with BRAF and Thyroid
 Skin Antibodies  >963 publications with BRAF and Skin
 Lung Antibodies  >437 publications with BRAF and Lung
 Blood Antibodies  >374 publications with BRAF and Blood
 Lymph Node Antibodies  >370 publications with BRAF and Lymph Node
 Brain Antibodies  >315 publications with BRAF and Brain
 Kidney Antibodies  >81 publications with BRAF and Kidney
 Prostate Antibodies  >56 publications with BRAF and Prostate
 Ovary Antibodies  >38 publications with BRAF and Ovary
 Pancreas Antibodies  >35 publications with BRAF and Pancreas
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