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anti-BTK antibody :: Rabbit BTK (Phospho-Tyr223) Antibody

Scan QR to view Datasheet Catalog #    MBS000637
Western blot
Unit / Price
0.05 mL  /  $270 +1 FREE 8GB USB
0.1 mL  /  $445 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

BTK (Phospho-Tyr223), Antibody

★Popular Item★
 Also Known As   

Rabbit BTK (Phospho-Tyr223) Antibody

 Product Synonym Names    Agammaglobulinaemia tyrosine kinase; AGMX1; ATK; B cell progenitor kinase; BPK; Bruton's tyrosine kinase; EC 2.7.10.2; kinase Btk; Kinase EMB
 Product Gene Name   

anti-BTK antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    MAAVILESIF LKRSQQKKKT SPLNFKKRLF LLTVHKLSYY EYDFERGRRG SKKGSIDVEK ITCVETVVPE KNPPPERQIP RRGEESSEME QISIIERFPY PFQVVYDEGP LYVFSPTEEL RKRWIHQLKN VIRYNSDLVQ KYHPCFWIDG QYLCCSQTAK NAMGCQILEN RNGSLKPGSS HRKTKKPLPP TPEEDQILKK PLPPEPAAAP VSTSELKKVV ALYDYMPMNA NDLQLRKGDE YFILEESNLP WWRARDKNGQ EGYIPSNYVT EAEDSIEMYE WYSKHMTRSQ AEQLLKQEGK EGGFIVRDSS KAGKYTVSVF AKSTGDPQGV IRHYVVCSTP QSQYYLAEKH LFSTIPELIN YHQHNSAGLI SRLKYPVSQQ NKNAPSTAGL GYGSWEIDPK DLTFLKELGT GQFGVVKYGK WRGQYDVAIK MIKEGSMSED EFIEEAKVMM NLSHEKLVQL YGVCTKQRPI FIITEYMANG CLLNYLREMR HRFQTQQLLE MCKDVCEAME YLESKQFLHR DLAARNCLVN DQGVVKVSDF GLSRYVLDDE YTSSVGSKFP VRWSPPEVLM YSKFSSKSDI WAFGVLMWEI YSLGKMPYER FTNSETAEHI AQGLRLYRPH LASEKVYTIM YSCWHEKADE RPTFKILLSN ILDVMDEES
 Chromosome Location    Chromosome: X; NC_000023.10 (100604435..100641212, complement). Location: Xq21.33-q22
 OMIM    300300
 3D Structure    ModBase 3D Structure for Q06187
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 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, mouse, rat
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 Specificity    Phospho-BTK(Tyr223) Antibody detects endogenous levels of BTK only when phosphorylated at Tyrosine 223.
 Purity/Purification    Immunogen affinity purified
 Form/Format    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
 Concentration    1 mg/ml (lot specific)
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 Immunogen    A synthesized peptide derived from human BTK around the phosphorylation site of Tyrosine 223.
 Storage buffer    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 C.Stable for 12 months from date of receipt.
 Subcellular Location    Cytoplasm,Cell membrane,Peripheral membrane proteinNucleus,Note:In steady state, BTK is predominantly cytosolic. Following B-cell receptor (BCR) engagement by antigen, translocates to the plasma membrane through its PH domain. Plasma membrane localization is a critical step in the activation of BTK. A fraction of BTK also shuttles between the nucleus and the cytoplasm, and nuclear export is mediated by the nuclear export receptor CRM1.
 Post-translational Modifications    Following B-cell receptor (BCR) engagement, translocates to the plasma membrane where it gets phosphorylated at Tyr-551 by LYN and SYK. Phosphorylation at Tyr-551 is followed by autophosphorylation of Tyr-223 which may create a docking site for a SH2 containing protein. Phosphorylation at Ser-180 by PRKCB, leads in translocation of BTK back to the cytoplasmic fraction. Phosphorylation at Ser-21 and Ser-115 creates a binding site for PIN1 at these Ser-Pro motifs, and promotes it's recruitment.
 Tissue Specificity    Predominantly expressed in B-lymphocytes.
 Subunit Structure    Binds GTF2I through the PH domain. Interacts with SH3BP5 via the SH3 domain. Interacts with IBTK via its PH domain. Interacts with ARID3A, CAV1, FASLG, PIN1, TLR8 and TLR9.
 Similarity    Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
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 Other Notes    Small volumes of anti-BTK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-BTK antibody

   Background: Defects in the Bruton tyrosine kinase (BTK) gene cause Agammaglobulinemia. Agammaglobulinemia is an X-linked immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement.

Function: Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.
 Applications Tested/Suitable for anti-BTK antibody   

Western Blot (WB)

 Application Notes for anti-BTK antibody    WB 1:500-1:2000
Optimal dilutions/concentrations should be determined by the end user.
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 Western blot of anti-BTK antibody    Western blot analysis on HeLa cell lysate using Phospho-BTK(Tyr223) antibody,The lane on the left is treated with the antigen-specific peptide.
anti-BTK antibody Western blot image
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NCBI/Uniprot data below describe general gene information for BTK. It may not necessarily be applicable to this product.
 NCBI GI #    4557377
 NCBI GeneID    695
 NCBI Accession #    NP_000052.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000061.2 [Other Products]
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 UniProt Primary Accession #    Q06187 [Other Products]
 UniProt Secondary Accession #    Q32ML5 [Other Products]
 UniProt Related Accession #    Q06187 [Other Products]
 Molecular Weight    80kDa
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 NCBI Official Full Name    tyrosine-protein kinase BTK
 NCBI Official Synonym Full Names    Bruton agammaglobulinemia tyrosine kinase
 NCBI Official Symbol    BTK [Similar Products]
 NCBI Official Synonym Symbols   
AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
[Similar Products]
 NCBI Protein Information    tyrosine-protein kinase BTK; B-cell progenitor kinase; agammaglobulinaemia tyrosine kinase; tyrosine-protein kinase BTK isoform (lacking exon 14); dominant-negative kinase-deficient Brutons tyrosine kinase
 UniProt Protein Name    Tyrosine-protein kinase BTK
 UniProt Synonym Protein Names   
Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase
 Protein Family    Tyrosine-protein kinase
 UniProt Gene Name    BTK [Similar Products]
 UniProt Synonym Gene Names    AGMX1; ATK; BPK; ATK; BPK [Similar Products]
 UniProt Entry Name    BTK_HUMAN
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 NCBI Summary for BTK    The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq, Nov 2008]
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 UniProt Comments for BTK    Btk: a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis.

Protein type: Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; Tec family

Chromosomal Location of Human Ortholog: Xq21.33-q22

Cellular Component: extrinsic to internal side of plasma membrane; mast cell granule; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; cytosol; lipid raft

Molecular Function: identical protein binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; metal ion binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; adaptive immune response; transcription, DNA-dependent; B cell activation; cell maturation; calcium-mediated signaling; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of cell proliferation; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; regulation of B cell cytokine production; MyD88-dependent toll-like receptor signaling pathway; regulation of B cell apoptosis; B cell receptor signaling pathway; histamine secretion by mast cell; toll-like receptor signaling pathway; innate immune response; positive regulation of B cell differentiation; mesoderm development; cell differentiation; toll-like receptor 4 signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Isolated Growth Hormone Deficiency, Type Iii; Agammaglobulinemia, X-linked
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 Research Articles on BTK    1. study reveals the existence of Btk-dependent molecular cooperation between FcgammaRIIa and TLR4 signaling cascades in LPS-"primed" human neutrophils
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-BTK antibodyPathways associated with anti-BTK antibody
 Reference Product  PubMed Publications
 TEC antibody  >190 publications with BTK and TEC
 SYK antibody  >154 publications with BTK and SYK
 LYN antibody  >117 publications with BTK and LYN
 ITK antibody  >82 publications with BTK and ITK
 BMX antibody  >48 publications with BTK and BMX
 CD19 antibody  >36 publications with BTK and CD19
 BLNK antibody  >35 publications with BTK and BLNK
 TLR4 antibody  >18 publications with BTK and TLR4
 CBL antibody  >15 publications with BTK and CBL
 FAS antibody  >13 publications with BTK and FAS
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway antibodies  Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 Class I PI3K Signaling Events Pathway antibodies  Class I PI3K Signaling Events Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
Diseases associated with anti-BTK antibodyOrgans/Tissues associated with anti-BTK antibody
 Disease Name  Pubmed Publications
 Agammaglobulinemia Antibodies  >699 publications with BTK and Agammaglobulinemia
 Neoplasms Antibodies  >125 publications with BTK and Neoplasms
 Inflammation Antibodies  >48 publications with BTK and Inflammation
 Cardiovascular Diseases Antibodies  >44 publications with BTK and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >34 publications with BTK and Disease Models, Animal
 Nervous System Diseases Antibodies  >21 publications with BTK and Nervous System Diseases
 Bruton type agammaglobulinemia Antibodies  >18 publications with BTK and Bruton type agammaglobulinemia
 Arteriosclerosis Antibodies  >10 publications with BTK and Arteriosclerosis
 Brain Diseases Antibodies  >10 publications with BTK and Brain Diseases
 Neoplasms, Experimental Antibodies  >9 publications with BTK and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >601 publications with BTK and Blood
 Bone Antibodies  >107 publications with BTK and Bone
 Bone Marrow Antibodies  >95 publications with BTK and Bone Marrow
 Spleen Antibodies  >84 publications with BTK and Spleen
 Lung Antibodies  >22 publications with BTK and Lung
 Liver Antibodies  >20 publications with BTK and Liver
 Skin Antibodies  >18 publications with BTK and Skin
 Lymph Node Antibodies  >16 publications with BTK and Lymph Node
 Heart Antibodies  >16 publications with BTK and Heart
 Brain Antibodies  >12 publications with BTK and Brain
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