Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow BTK  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • CC Chemokine Receptor 5 (CCR5) ELISA Kit
 • PIN1 Antibody
 • Ly6a Antibody
 • PAPPA Native Protein
 • A Disintegrin and Metalloprotease 22 (ADAM22) ELISA Kit
 • Apolipoprotein B (APOB) ELISA Kit
 • Prestin (SLC26A5) ELISA Kit
 • Total Immunoglobulin (TIG) ELISA Kit
 • NMDA NR2B Subunit, N-terminus (GRIN2B) Antibody
 • CD279 Antibody
 • DDI2 Antibody
 • TrkB Antibody
 • MKP-2 Antibody
 • ABCF2 Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-BTK antibody :: anti-Human, Mouse BTK Antibody

Scan QR to view Datasheet Catalog #    MBS852725
Unit / Price
0.1 mg  /  $215 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

BTK, Antibody

★Popular Item★
 Also Known As   

BTK (Ab-222) Antibody

 Product Synonym Names    AGMX1; ATK; Agammaglobulinaemia tyrosine kinase; B cell progenitor kinase; BPK; Bruton's tyrosine kinase; EC 2.7.10.2; Kinase EMB; kinase Btk
 Product Gene Name   

anti-BTK antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    300300
 3D Structure    ModBase 3D Structure for Q06187
Section Bar
 Species Reactivity    Human, Mouse
Section Bar
 Specificity    BTK (Ab-222) antibody detects endogenous levels of total BTK protein.
 Purity/Purification    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
 Form/Format    In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Section Bar
 Immunogen    The antiserum was produced against synthesized non-phosphopeptide derived from human BTK around the phosphorylation site of tyrosine 222 (A-L-YP-D-Y).
Section Bar
 Preparation and Storage    Store at-20 degree C for 1 year
 Other Notes    Small volumes of anti-BTK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 Product Categories/Family for anti-BTK antibody    Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
 Applications Tested/Suitable for anti-BTK antibody   

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

 Application Notes for anti-BTK antibody    WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:20000
Section Bar
 Immunohistochemistry (IHC) of anti-BTK antibody    Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BTK (Ab-222) antibody.
anti-BTK antibody Immunohistochemistry (IHC) (IHC) image
Section Bar
 Western Blot (WB) of anti-BTK antibody    Western blot analysis of extracts from Hela cells, using BTK (Ab-222) antibody.
anti-BTK antibody Western Blot (WB) (WB) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for BTK. It may not necessarily be applicable to this product.
 NCBI GI #    4557377
 NCBI GeneID    695
 NCBI Accession #    NP_000052.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000061.2 [Other Products]
Section Bar
 UniProt Primary Accession #    Q06187 [Other Products]
 UniProt Secondary Accession #    Q32ML5; B2RAW1 [Other Products]
 UniProt Related Accession #    Q06187 [Other Products]
 Molecular Weight    79,937 Da
Table BarTOPTable Bar
 NCBI Official Full Name    tyrosine-protein kinase BTK isoform 1
 NCBI Official Synonym Full Names    Bruton tyrosine kinase
 NCBI Official Symbol    BTK [Similar Products]
 NCBI Official Synonym Symbols   
AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
[Similar Products]
 NCBI Protein Information    tyrosine-protein kinase BTK
 UniProt Protein Name    Tyrosine-protein kinase BTK
 UniProt Synonym Protein Names   
Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase
 Protein Family    Tyrosine-protein kinase
 UniProt Gene Name    BTK [Similar Products]
 UniProt Synonym Gene Names    AGMX1; ATK; BPK; ATK; BPK [Similar Products]
Section Bar
 NCBI Summary for BTK    The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]
Section Bar
 UniProt Comments for BTK    Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.
Table BarTOPTable Bar
 

Product References and Citations for anti-BTK antibody

   Sulekha Verma, J. Biol. Chem., Feb 2001; 276: 4671-4676. Yan M. Li, Cancer Res., Nov 2003; 63: 7630-7633. Malti Nikrad, Mol. Cancer Ther., Mar 2005; 4: 443-449. Feng Dong, Infect. Immun., Mar 2005; 73: 1861-1864. Hiroo Ueno, Mol. Biol. Cell, Feb 2000; 11: 735. Hidenori Hase, J. Biol. Chem., Nov 2002; 277: 46950-46958. Yasuyuki Hirashima, J. Biol. Chem., Jul 2003; 278: 26793-26802. Mingzhong Yao, J. Neurosci., Feb 2007; 27: 1422-1433.
Section Bar
 Research Articles on BTK    1. Ibrutinib-resistant TMD8 cells had higher BCL2 gene expression and increased sensitivity to ABT-199, a BCL-2 inhibitor. Consistently, clinical samples from ABC-DLBCL patients who experienced poorer response to ibrutinib had higher BCL2 gene expression. We further demonstrated synergistic growth suppression by ibrutinib and ABT-199 in multiple ABC-DLBCL, GCB-DLBCL, and follicular lymphoma cell lines.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-BTK antibodyPathways associated with anti-BTK antibody
 Reference Product  PubMed Publications
 TEC antibody  >190 publications with BTK and TEC
 SYK antibody  >154 publications with BTK and SYK
 LYN antibody  >117 publications with BTK and LYN
 ITK antibody  >82 publications with BTK and ITK
 BMX antibody  >48 publications with BTK and BMX
 CD19 antibody  >36 publications with BTK and CD19
 BLNK antibody  >35 publications with BTK and BLNK
 TLR4 antibody  >18 publications with BTK and TLR4
 CBL antibody  >15 publications with BTK and CBL
 FAS antibody  >13 publications with BTK and FAS
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway antibodies  Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 Class I PI3K Signaling Events Pathway antibodies  Class I PI3K Signaling Events Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
Diseases associated with anti-BTK antibodyOrgans/Tissues associated with anti-BTK antibody
 Disease Name  Pubmed Publications
 Agammaglobulinemia Antibodies  >699 publications with BTK and Agammaglobulinemia
 Neoplasms Antibodies  >125 publications with BTK and Neoplasms
 Inflammation Antibodies  >48 publications with BTK and Inflammation
 Cardiovascular Diseases Antibodies  >44 publications with BTK and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >34 publications with BTK and Disease Models, Animal
 Nervous System Diseases Antibodies  >21 publications with BTK and Nervous System Diseases
 Bruton type agammaglobulinemia Antibodies  >18 publications with BTK and Bruton type agammaglobulinemia
 Arteriosclerosis Antibodies  >10 publications with BTK and Arteriosclerosis
 Brain Diseases Antibodies  >10 publications with BTK and Brain Diseases
 Neoplasms, Experimental Antibodies  >9 publications with BTK and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >601 publications with BTK and Blood
 Bone Antibodies  >107 publications with BTK and Bone
 Bone Marrow Antibodies  >95 publications with BTK and Bone Marrow
 Spleen Antibodies  >84 publications with BTK and Spleen
 Lung Antibodies  >22 publications with BTK and Lung
 Liver Antibodies  >20 publications with BTK and Liver
 Skin Antibodies  >18 publications with BTK and Skin
 Lymph Node Antibodies  >16 publications with BTK and Lymph Node
 Heart Antibodies  >16 publications with BTK and Heart
 Brain Antibodies  >12 publications with BTK and Brain
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions