Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody  rightarrow PTPRC rightarrow Monoclonal PTPRC  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • GIRK1-KIR3.1-KCNJ3 (Phospho-Ser185) Antibody
 • Recombination Activating Gene 2 (PAG-2) ELISA Kit
 • Crystal Proteins beta (CRYbeta) ELISA Kit
 • Protein Tyrosine Phosphatase Receptor Type N (PTPRN) ELISA Kit
 • Major Histocompatibility Complex I (MHC 1) ELISA Kit
 • Mucin-5 Subtype Ac (MUC5AC) ELISA Kit
 • sCD8 ELISA Kit
 • Nucleoporin 37Kda (NUP37) ELISA Kit
 • Arachidonic Acid (AA) ELISA Kit
 • Fas-Apo-1 ELISA Kit
 • Acetylcholine Receptor Antibody (ACHRAB) ELISA Kit
 • Thyroid-Globulin Antibody (TG) ELISA Kit
 • Peroxiredoxin-2 (PRDX2) ELISA Kit
 • Fibrinopeptide B (FPB) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

PTPRC antibody :: CD45RO Antibody

Scan QR to view Datasheet Catalog #    MBS438407
Immunohistochemistry (IHC)
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $150 +1 FREE 8GB USB
7 ml (Prediluted)  /  $270 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $270 +1 FREE 8GB USB
0.1 mg (Without BSA & Azide at 1mg/ml)  /  $285 +1 FREE 8GB USB
0.2 mg (With BSA & Azide at 0.2mg/ml)  /  $360 +1 FREE 8GB USB
0.2 mg (Without BSA & Azide at 1mg/ml)  /  $380 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD45RO (PTPRC), Antibody

★Popular Item★
 Also Known As   

CD45RO (T-Cell Marker) Mouse Monoclonal Antibody

 Product Synonym Names    T200, GP180, LCA, Leukocyte Common Antigen, LY5, Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
 Product Gene Name   

PTPRC antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    126200
 3D Structure    ModBase 3D Structure for P08575
Section Bar
 Clonality    Monoclonal
 Isotype    IgG2a, kappa
 Clone Number    SPM125
 Host    Mouse
 Species Reactivity    Human, Chimpanzee, Common Marmoset
Section Bar
 Specificity    Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
 Form/Format    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Section Bar
 Cellular Localization    Cell Surface
 Immunogen    Cultured human T-cells from an IL-2-dependent T-cell line (CA1)
 Hu-Chromosome Location    1q31.3
 Positive Control    CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
Section Bar
 Preparation and Storage    Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
 Other Notes    Small volumes of PTPRC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 Applications Tested/Suitable for PTPRC antibody   

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

 Application Notes for PTPRC antibody    Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Section Bar
 Immunohistochemistry (IHC) of PTPRC antibody    Formalin-fixed, paraffin-embedded human Lymphoma stained with CD45RO Monoclonal Antibody (SPM125).
PTPRC antibody  Immunohistochemistry (IHC) (IHC) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for PTPRC. It may not necessarily be applicable to this product.
 NCBI GI #    392307007
 NCBI GeneID    5788
 NCBI Accession #    NP_002829.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_002838.4 [Other Products]
Section Bar
 UniProt Primary Accession #    P08575 [Other Products]
 UniProt Secondary Accession #    Q16614; Q9H0Y6; A8K7W6 [Other Products]
 UniProt Related Accession #    P08575 [Other Products]
 Molecular Weight    180-185kDa
Table BarTOPTable Bar
 NCBI Official Full Name    receptor-type tyrosine-protein phosphatase C isoform 1
 NCBI Official Synonym Full Names    protein tyrosine phosphatase, receptor type C
 NCBI Official Symbol    PTPRC [Similar Products]
 NCBI Official Synonym Symbols   
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
[Similar Products]
 NCBI Protein Information    receptor-type tyrosine-protein phosphatase C
 UniProt Protein Name    Receptor-type tyrosine-protein phosphatase C
 UniProt Synonym Protein Names   
Leukocyte common antigen; L-CA; T200; CD_antigen: CD45
 Protein Family    Receptor-type tyrosine-protein phosphatase
 UniProt Gene Name    PTPRC [Similar Products]
 UniProt Synonym Gene Names    CD45; L-CA [Similar Products]
 UniProt Entry Name    PTPRC_HUMAN
Section Bar
 NCBI Summary for PTPRC    The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
Section Bar
 UniProt Comments for PTPRC    CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Receptor protein phosphatase, tyrosine; Membrane protein, integral; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 1q31-q32

Cellular Component: cell surface; external side of plasma membrane; focal adhesion; integral to plasma membrane; membrane; plasma membrane

Molecular Function: protein binding; protein kinase binding; protein tyrosine phosphatase activity; transmembrane receptor protein tyrosine phosphatase activity

Biological Process: B cell proliferation; B cell receptor signaling pathway; bone marrow development; cell surface receptor linked signal transduction; defense response to virus; dephosphorylation; hemopoietic progenitor cell differentiation; immunoglobulin biosynthetic process; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of protein kinase activity; negative regulation of T cell mediated cytotoxicity; positive regulation of antigen receptor-mediated signaling pathway; positive regulation of B cell proliferation; positive regulation of T cell proliferation; protein amino acid dephosphorylation; regulation of cell cycle; release of sequestered calcium ion into cytosol; stem cell development; substrate-bound cell migration, cell release from substrate; T cell differentiation; T cell receptor signaling pathway

Disease: Hepatitis C Virus, Susceptibility To; Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-positive
Table BarTOPTable Bar
 

Product References and Citations for PTPRC antibody

   Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.2. Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138.3. Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315.4. Yamada M, et al. 1996. Stroke 27:1155. 5. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430
Section Bar
 Research Articles on PTPRC    1. Findings indicate that CD45 antigen(+) and c-Kit protein(+) hematopoietic cells were more abundant in muscle than in bone marrow between embryonic 14.5 and 17.5 days.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with PTPRC antibody Pathways associated with PTPRC antibody
 Reference Product  PubMed Publications
 CD4 antibody  >109 publications with PTPRC and CD4
 LCK antibody  >22 publications with PTPRC and LCK
 ZAP70 antibody  >10 publications with PTPRC and ZAP70
 CSK antibody  >7 publications with PTPRC and CSK
 FYN antibody  >5 publications with PTPRC and FYN
 CD22 antibody  >4 publications with PTPRC and CD22
 ITGAM antibody  >4 publications with PTPRC and ITGAM
 LYN antibody  >3 publications with PTPRC and LYN
 LGALS1 antibody  >2 publications with PTPRC and LGALS1
 SPI1 antibody  >1 publications with PTPRC and SPI1
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPO Receptor Signaling Pathway antibodies  EPO Receptor Signaling Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
Diseases associated with PTPRC antibody Organs/Tissues associated with PTPRC antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >89 publications with PTPRC and Inflammation
 Cardiovascular Diseases Antibodies  >79 publications with PTPRC and Cardiovascular Diseases
 Necrosis Antibodies  >46 publications with PTPRC and Necrosis
 Lung Diseases Antibodies  >30 publications with PTPRC and Lung Diseases
 Fibrosis Antibodies  >26 publications with PTPRC and Fibrosis
 Breast Neoplasms Antibodies  >22 publications with PTPRC and Breast Neoplasms
 Arthritis, Rheumatoid Antibodies  >21 publications with PTPRC and Arthritis, Rheumatoid
 Multiple Sclerosis Antibodies  >20 publications with PTPRC and Multiple Sclerosis
 Metaplasia Antibodies  >15 publications with PTPRC and Metaplasia
 Multiple Myeloma Antibodies  >14 publications with PTPRC and Multiple Myeloma
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >462 publications with PTPRC and Blood
 Bone Antibodies  >161 publications with PTPRC and Bone
 Bone Marrow Antibodies  >151 publications with PTPRC and Bone Marrow
 Vascular Antibodies  >97 publications with PTPRC and Vascular
 Brain Antibodies  >58 publications with PTPRC and Brain
 Liver Antibodies  >56 publications with PTPRC and Liver
 Lung Antibodies  >51 publications with PTPRC and Lung
 Muscle Antibodies  >49 publications with PTPRC and Muscle
 Embryonic Tissue Antibodies  >40 publications with PTPRC and Embryonic Tissue
 Spleen Antibodies  >39 publications with PTPRC and Spleen
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions