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anti-CHEK1 antibody :: Rabbit anti-Human, Mouse CHEK1-S280 Antibody

Scan QR to view Datasheet Catalog #    MBS125258
Unit / Price
0.05 mL  /  $265 +1 FREE 8GB USB
0.1 mL  /  $380 +1 FREE 8GB USB
0.2 mL  /  $555 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CHEK1-S280, Antibody

 Also Known As   

Phospho-CHEK1-S280 Polyclonal Antibody

 Product Synonym Names    Phospho-CHEK1-S280: CHK1
 Product Gene Name   

anti-CHEK1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    603078
 3D Structure    ModBase 3D Structure for O14757
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 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse
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 Purity/Purification    Affinity Purification
 Concentration    1mg/ml (lot specific)
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 Species    Human
 Route    Synthetic Peptide
 Immunogen    A phospho specific peptide corresponding to residues surrounding S280 of human CHEK1
 Calculated Molecular Weight    54kDa
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 Preparation and Storage    Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
 Other Notes    Small volumes of anti-CHEK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CHEK1 antibody

   The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
 Product Categories/Family for anti-CHEK1 antibody    Phosphorylation
 Applications Tested/Suitable for anti-CHEK1 antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

 Application Notes for anti-CHEK1 antibody    WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
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 Western Blot (WB) of anti-CHEK1 antibody    Western blot analysis of extracts of HT-29 cells, using Phospho-CHEK1-Ser280 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
anti-CHEK1 antibody Western Blot (WB) (WB) image
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 Immunofluorescence (IF) of anti-CHEK1 antibody    Immunofluorescence analysis of MCF-7 cells using Phospho-CHEK1-S280 antibody. Blue: DAPI for nuclear staining.
anti-CHEK1 antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for CHEK1. It may not necessarily be applicable to this product.
 NCBI GI #    317373343
 NCBI GeneID    1111
 NCBI Accession #    O14757.2 [Other Products]
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 UniProt Primary Accession #    O14757 [Other Products]
 UniProt Secondary Accession #    A8K934; B4DDD0; B4DSK3; B5BTY6; F5H7S4; H2BI51 [Other Products]
 UniProt Related Accession #    O14757 [Other Products]
 Molecular Weight    476
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 NCBI Official Full Name    Serine/threonine-protein kinase Chk1
 NCBI Official Synonym Full Names    checkpoint kinase 1
 NCBI Official Symbol    CHEK1 [Similar Products]
 NCBI Official Synonym Symbols   
CHK1
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase Chk1; Chk1-S; checkpoint kinase-1; CHK1 checkpoint homolog; cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1
 UniProt Protein Name    Serine/threonine-protein kinase Chk1
 UniProt Synonym Protein Names   
CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1
 UniProt Gene Name    CHEK1 [Similar Products]
 UniProt Synonym Gene Names    CHK1 [Similar Products]
 UniProt Entry Name    CHK1_HUMAN
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 NCBI Summary for CHEK1    The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]
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 UniProt Comments for CHEK1    Chk1: a protein kinase of the CAMKL family. Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Plays an important role in both S and G2 checkpoints, embryonic development and tumor suppression. Phosphorylated and activated by ATM/ATR following DNA damage, it binds to single-stranded DNA and DNA ends and has a role in the repair of double strand breaks. Activated Chk1 can phosphorylate and inactivate cdc25C via phosphorylation and 14-3-3 binding, blocking the activation of cdc2 and transition into M-phase. Other substrates include p53. Implicated in resistance to apoptosis in response to chemotherapy. Inhibitors under development to chemosensitize tumors. Somatic mutations found in stomach tumors, and in colon and endometrial tumors, where CHK1 may be a target of microsatellite instability. Inhibitors: SB218078, UNC-01.

Protein type: Kinase, protein; Tumor suppressor; Protein kinase, CAMK; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMKL family; CHK1 subfamily

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: nucleoplasm; centrosome; extracellular space; chromosome, telomeric region; condensed nuclear chromosome; intracellular membrane-bound organelle; replication fork; cytosol; nucleus; chromatin

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: DNA damage induced protein phosphorylation; chromatin-mediated maintenance of transcription; regulation of mitotic centrosome separation; negative regulation of mitosis; DNA damage checkpoint; peptidyl-threonine phosphorylation; G2/M transition DNA damage checkpoint; G2/M transition of mitotic cell cycle; DNA repair; DNA replication; response to DNA damage stimulus; regulation of cell proliferation
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 Research Articles on CHEK1    1. selective targeting of Chk1 and Chk2 by oncolytic adenovirus mutants was chosen to treat resistant tumor xenograft mice, and the maximum antitumoral efficacy was achieved with the combined co-abrogation of Chk1 and Chk2
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CHEK1 antibodyPathways associated with anti-CHEK1 antibody
 Reference Product  PubMed Publications
 ATR antibody  >20 publications with CHEK1 and ATR
 ATM antibody  >19 publications with CHEK1 and ATM
 TP53 antibody  >12 publications with CHEK1 and TP53
 RAD51 antibody  >8 publications with CHEK1 and RAD51
 CDC25A antibody  >4 publications with CHEK1 and CDC25A
 CDC25C antibody  >2 publications with CHEK1 and CDC25C
 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chk1/Chk2(Cds1) Mediated Inactivation Of Cyclin B:Cdk1 Complex Pathway antibodies  Chk1/Chk2(Cds1) Mediated Inactivation Of Cyclin B:Cdk1 Complex Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
 DNA Damage Response Pathway antibodies  DNA Damage Response Pathway Diagram
 DNA Damage-induced Cell Cycle Checkpoints Pathway antibodies  DNA Damage-induced Cell Cycle Checkpoints Pathway Diagram
Diseases associated with anti-CHEK1 antibodyOrgans/Tissues associated with anti-CHEK1 antibody
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >9 publications with CHEK1 and Breast Neoplasms
 Lung Neoplasms Antibodies  >6 publications with CHEK1 and Lung Neoplasms
 Adenocarcinoma Antibodies  >5 publications with CHEK1 and Adenocarcinoma
 Liver Neoplasms Antibodies  >5 publications with CHEK1 and Liver Neoplasms
 Liver Diseases Antibodies  >4 publications with CHEK1 and Liver Diseases
 Colonic Neoplasms Antibodies  >4 publications with CHEK1 and Colonic Neoplasms
 Neoplasms, Experimental Antibodies  >3 publications with CHEK1 and Neoplasms, Experimental
 Fatty Liver Antibodies  >3 publications with CHEK1 and Fatty Liver
 Carcinoma, Hepatocellular Antibodies  >3 publications with CHEK1 and Carcinoma, Hepatocellular
 Cell Transformation, Neoplastic Antibodies  >3 publications with CHEK1 and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >8 publications with CHEK1 and Lung
 Liver Antibodies  >8 publications with CHEK1 and Liver
 Blood Antibodies  >7 publications with CHEK1 and Blood
 Lymph Node Antibodies  >6 publications with CHEK1 and Lymph Node
 Bone Antibodies  >6 publications with CHEK1 and Bone
 Bone Marrow Antibodies  >5 publications with CHEK1 and Bone Marrow
 Prostate Antibodies  >4 publications with CHEK1 and Prostate
 Embryonic Tissue Antibodies  >3 publications with CHEK1 and Embryonic Tissue
 Lymph Antibodies  >3 publications with CHEK1 and Lymph
 Kidney Antibodies  >3 publications with CHEK1 and Kidney
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