View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Flavivirus group antigen Recombinant Antibody Catalog # MBS488169 Unit / Price 0.2 mg / $570 +1 FREE 8GB USB 5x0.2 mg / $2,370 +2 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Flavivirus group antigen, Recombinant Antibody Popular Item Full Product Name Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)] Product Synonym Names Protein E; Envelope Protein Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP MBS488169 COA COA PDF MBS488169 MSDS MSDS PDF MBS488169 Technical Datasheet Technical Datasheet PDF Isotype Mouse IgGc2a, kappa Clone Number D1-4G2-4-15 (4G2) Species Reactivity Dengue Virus, Flaviviridae, West Nile Virus, Yellow Fever Virus, Zika Virus Specificity Recognizes flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E. Purity/Purification Protein A Affinity Purified Form/Format PBS only with 0.02% Proclin 300. Concentration 1 mg/ml (lot specific) Immunogen Dengue Virus type 2 antigens Preparation and Storage Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. ISO Certification Manufactured in an ISO 9001:2015 Certified Laboratory. Animal-free Manufacturing Platform The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free. Other Notes Small volumes of Flavivirus group antigen recombinant antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Product Categories/Family for Flavivirus group antigen recombinant antibody Infectious diseases; Virus Applications Tested/Suitable for Flavivirus group antigen recombinant antibody Immunohistochemistry-Paraffin (IHC-P), Neutralization (NTRL), Western Blot (WB), ELISA, Flow Cytometry (FC), Immunofluoresence (IF) Application Notes for Flavivirus group antigen recombinant antibody This antibody binds to flavivirus group antigen, protein E. It can be used as an anti-Dengue virus antibody, anti-West Nile virus antibody, anti-Japanese Encephalitis or anti-Zika Virus antibody (Aubry et al. 2016) to identify cells infected with these flaviviridae. It binds to the fusion loop at the extremity of domain II of E protein from all four serotypes and prevents syncytia formation (Summers, 1989). The epitope is highly conserved amongst flaviviridae and has been functionally analyzed in detail by Crill and Chang 2004 (PMID: 15564505). Previous studies have used acetone- (Henchal et al. 1982) or methanol-fixed slides (Moreland & Tay, 2010). Please note that binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions (Lai et al. 2008). A version of 4G2 directly conjugaed to APC was used by Quicke and co-workers (Quicke, 2016) to detect Zika virus in human placental macrophages by flow-cytometry (Quicke 2016). Ramaiah et al. (2016) used Ab00230-2.0 at a 1:200 dilution to stain for Zika virus in methanol-fixed Vero cells 48 h post-infection. TOP Product References and Citations for Flavivirus group antigen recombinant antibody Nawa et al. Development of dengue IgM-capture enzyme-linked immunosorbent assay with higher sensitivity using monoclonal detection antibody. J Virol Methods PMID:11164919 TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP