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anti-H3K27ac antibody :: Rabbit H3K27AC Antibody

Scan QR to view Datasheet Catalog #    MBS9126998
Western Blot (WB)
Unit / Price
0.05 mL  /  $265 +1 FREE 8GB USB
0.1 mL  /  $380 +1 FREE 8GB USB
0.2 mL  /  $555 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

H3K27AC, Antibody

★Popular Item★
 Also Known As   

Histone H3K27AC Polyclonal Antibody

 Product Synonym Names    Acetyl-Histone H3-K27: H3t; H3.4; H3/g; H3FT
 Product Gene Name   

anti-H3K27ac antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    602820
 3D Structure    ModBase 3D Structure for Q16695
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 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat, Other (Wide Range)
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 Purity/Purification    Affinity Purification
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 Species    Human
 Route    Synthetic Peptide
 Immunogen    A synthetic acetylated peptide corresponding to residues surrounding K27 of human Histone H3
 Calculated Molecular Weight    16kDa
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 Preparation and Storage    Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
 Other Notes    Small volumes of anti-H3K27ac antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-H3K27ac antibody

   Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
 Product Categories/Family for anti-H3K27ac antibody    Acetylation
 Applications Tested/Suitable for anti-H3K27ac antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP, CHIPseq

 Application Notes for anti-H3K27ac antibody    WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
CHIP: 1:20 - 1:100
CHIPseq: 1:20 - 1:100
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 Western Blot (WB) of anti-H3K27ac antibody    Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K27 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
anti-H3K27ac antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for H3K27ac. It may not necessarily be applicable to this product.
 NCBI GI #    18202512
 NCBI GeneID    8290
 NCBI Accession #    Q16695.3 [Other Products]
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 UniProt Primary Accession #    Q16695 [Other Products]
 UniProt Secondary Accession #    Q6FGU4; B2R5K3 [Other Products]
 UniProt Related Accession #    Q16695 [Other Products]
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 NCBI Official Full Name    Histone H3.1t
 NCBI Official Synonym Full Names    histone cluster 3, H3
 NCBI Official Symbol    HIST3H3 [Similar Products]
 NCBI Official Synonym Symbols   
H3t; H3.4; H3/g; H3FT
[Similar Products]
 NCBI Protein Information    histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
 UniProt Protein Name    Histone H3.1t
 UniProt Synonym Protein Names   
H3/g
 UniProt Gene Name    HIST3H3 [Similar Products]
 UniProt Synonym Gene Names    H3FT; H3/t; H3t [Similar Products]
 UniProt Entry Name    H31T_HUMAN
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 NCBI Summary for H3K27ac    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
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 UniProt Comments for H3K27ac    HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: negative regulation of protein oligomerization; nucleosome assembly; protein heterotetramerization; telomere maintenance
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 Research Articles on H3K27ac    1. Deregulation of histone H3 trimethylation at lysine 27 in diffuse large B-cell lymphoma may be another high-risk phenotype
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-H3K27ac antibodyDiseases associated with anti-H3K27ac antibody
 Products by Pathway  Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 Condensation Of Prophase Chromosomes Pathway antibodies  Condensation Of Prophase Chromosomes Pathway Diagram
 DNA Damage/Telomere Stress Induced Senescence Pathway antibodies  DNA Damage/Telomere Stress Induced Senescence Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >1 publications with H3K27ac and Fibrosis
 Cell Transformation, Neoplastic Antibodies  >1 publications with H3K27ac and Cell Transformation, Neoplastic
 Necrosis Antibodies  >1 publications with H3K27ac and Necrosis
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