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anti-H3K9me3 antibody :: H3K9me3 Antibody

Scan QR to view Datasheet Catalog #    MBS126185
Unit / Price
0.05 mL  /  $265 +1 FREE 8GB USB
0.1 mL  /  $380 +1 FREE 8GB USB
0.2 mL  /  $555 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

H3K9me3, Antibody

★Popular Item★
 Also Known As   

Histone H3K9me3 Polyclonal Antibody

 Product Synonym Names    TriMethyl-Histone H3-K9: HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3
 Product Gene Name   

anti-H3K9me3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    602820
 3D Structure    ModBase 3D Structure for Q16695
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 Isotype    IgG
 Species Reactivity    Human, Mouse, Rat, Other (Wide Range)
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 Purity/Purification    Affinity Purification
 Concentration    1mg/ml (lot specific)
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 Source    Rabbit
 Buffer    PBS with 0.02% sodium azide, 50% glycerol, pH7.3
    
 Immunogen    A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
    
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 Preparation and Storage    Store at -20 degree C. Avoid freeze / thaw cycles.
 Other Notes    Small volumes of anti-H3K9me3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-H3K9me3 antibody

   Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
 Product Categories/Family for anti-H3K9me3 antibody    Methylation
 Applications Tested/Suitable for anti-H3K9me3 antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP, CHIPseq

 Application Notes for anti-H3K9me3 antibody    WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
ChIPseq: 1:50 - 1:200
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 Western Blot (WB) of anti-H3K9me3 antibody    Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
anti-H3K9me3 antibody Western Blot (WB) (WB) image
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 Dot Blot (DB) of anti-H3K9me3 antibody    Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K9 antibody.
anti-H3K9me3 antibody Dot Blot (DB) image
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 Immunohistochemistry (IHC) of anti-H3K9me3 antibody    Immunohistochemistry of paraffin-embedded rat lung using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).
anti-H3K9me3 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-H3K9me3 antibody    Immunohistochemistry of paraffin-embedded human kidney using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).
anti-H3K9me3 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-H3K9me3 antibody    Immunohistochemistry of paraffin-embedded human lung cancer using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).
anti-H3K9me3 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-H3K9me3 antibody    Immunohistochemistry of paraffin-embedded human liver cancer using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).
anti-H3K9me3 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-H3K9me3 antibody    Immunohistochemistry of paraffin-embedded Mouse brain using TriMethyl-Histone H3-K9 antibody at dilution of 1:100 (40x lens).
anti-H3K9me3 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-H3K9me3 antibody    Immunohistochemistry of paraffin-embedded Mouse lung using TriMethyl-Histone H3-K9 antibody at dilution of 1:100 (40x lens).
anti-H3K9me3 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-H3K9me3 antibody    Immunohistochemistry of paraffin-embedded Rat brain using TriMethyl-Histone H3-K9 antibody at dilution of 1:100 (40x lens).
anti-H3K9me3 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for H3K9me3. It may not necessarily be applicable to this product.
 NCBI GI #    18202512
 NCBI GeneID    8290
 NCBI Accession #    Q16695.3 [Other Products]
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 UniProt Primary Accession #    Q16695 [Other Products]
 UniProt Secondary Accession #    Q6FGU4; B2R5K3 [Other Products]
 UniProt Related Accession #    Q16695 [Other Products]
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 NCBI Official Full Name    Histone H3.1t
 NCBI Official Synonym Full Names    histone cluster 3, H3
 NCBI Official Symbol    HIST3H3 [Similar Products]
 NCBI Official Synonym Symbols   
H3t; H3.4; H3/g; H3FT
[Similar Products]
 NCBI Protein Information    histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
 UniProt Protein Name    Histone H3.1t
 UniProt Synonym Protein Names   
H3/g
 UniProt Gene Name    HIST3H3 [Similar Products]
 UniProt Synonym Gene Names    H3FT; H3/t; H3t [Similar Products]
 UniProt Entry Name    H31T_HUMAN
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 NCBI Summary for H3K9me3    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
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 UniProt Comments for H3K9me3    HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance
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 Research Articles on H3K9me3    1. Deregulation of histone H3 trimethylation at lysine 27 in diffuse large B-cell lymphoma may be another high-risk phenotype
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-H3K9me3 antibodyDiseases associated with anti-H3K9me3 antibody
 Products by Pathway  Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 Condensation Of Prophase Chromosomes Pathway antibodies  Condensation Of Prophase Chromosomes Pathway Diagram
 DNA Damage/Telomere Stress Induced Senescence Pathway antibodies  DNA Damage/Telomere Stress Induced Senescence Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >1 publications with H3K9me3 and Fibrosis
 Cell Transformation, Neoplastic Antibodies  >1 publications with H3K9me3 and Cell Transformation, Neoplastic
 Necrosis Antibodies  >1 publications with H3K9me3 and Necrosis
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