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IDH1 antibody :: IDH1 (Isocitrate Dehydrogenase) Antibody

Scan QR to view Datasheet Catalog #    MBS439694
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IDH1 (Isocitrate Dehydrogenase), Antibody

★Popular Item★
 Also Known As   

IDH1 (Isocitrate Dehydrogenase) Mouse Monoclonal Antibody

 Product Synonym Names    Cytosolic NADP-isocitrate dehydrogenase; Epididymis luminal protein 216; Epididymis secretory protein Li 26; HEL-216; HEL-S-26; ICDH; IDCD; IDH1; IDP; IDPC; Isocitrate dehydrogenase [NADP] cytoplasmic; Isocitrate dehydrogenase 1 (NADP+) soluble; NADP dependent isocitrate dehydrogenase peroxisomal; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; PICD
 Product Gene Name   

IDH1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    137800
 3D Structure    ModBase 3D Structure for O75874
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 Clonality    Monoclonal
 Isotype    IgG1, kappa
 Clone Number    IDH1/1152
 Host    Mouse
 Species Reactivity    Human.
Others not known
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 Specificity    It recognizes a 45kDa protein, which is identified as isocitrate dehydrogenase (IDH1). It belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming alpha-ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of alpha-ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.
 Form/Format    7.0ml of Ready-to-Use for IHC staining of formalin-fixed tissues.
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 Cellular Localization    Cytoplasmic & Nuclear
 Immunogen    Recombinant fragment (119 Amino acid residues around aa 280-420) of human IDH1 protein
 Hu-Chromosome Location    2q33.3
 Positive Control    HepG2, HeLa, HT29 or MCF7 cells. Breast, Colon or Prostate Carcinoma.
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 Preparation and Storage    Store at 2 to 8 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
 Other Notes    Small volumes of IDH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for IDH1 antibody   

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

 Application Notes for IDH1 antibody    Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Western Blot (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (Incubate for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
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 Immunohistochemistry (IHC) of IDH1 antibody    Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with IDH1 Monoclonal Antibody (IDH1/1152).
IDH1 antibody  Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of IDH1 antibody    Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with IDH1 Monoclonal Antibody (IDH1/1152).
IDH1 antibody  Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for IDH1. It may not necessarily be applicable to this product.
 NCBI GI #    538917488
 NCBI GeneID    3417
 NCBI Accession #    NP_001269315.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001282386.1 [Other Products]
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 UniProt Primary Accession #    O75874 [Other Products]
 UniProt Secondary Accession #    Q567U4; Q6FHQ6; Q7Z3V0; Q93090; Q9NTJ9; Q9UKW8 [Other Products]
 UniProt Related Accession #    O75874 [Other Products]
 Molecular Weight    45-47kDa
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 NCBI Official Full Name    isocitrate dehydrogenase
 NCBI Official Synonym Full Names    isocitrate dehydrogenase (NADP(+)) 1, cytosolic
 NCBI Official Symbol    IDH1 [Similar Products]
 NCBI Official Synonym Symbols   
IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
[Similar Products]
 NCBI Protein Information    isocitrate dehydrogenase [NADP] cytoplasmic
 UniProt Protein Name    Isocitrate dehydrogenase [NADP] cytoplasmic
 UniProt Synonym Protein Names   
Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase
 Protein Family    Isocitrate dehydrogenase
 UniProt Gene Name    IDH1 [Similar Products]
 UniProt Synonym Gene Names    PICD; IDH [Similar Products]
 UniProt Entry Name    IDHC_HUMAN
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 NCBI Summary for IDH1    Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]
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 UniProt Comments for IDH1    IDH1: an oxidoreductase that catalyzes the third step of the TCA cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH1 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. IDH1 is located in the cytosol and peroxisomes. Somatic mutations affecting arginine 132 (R132) are found in 80% of grade II?III gliomas and secondary glioblastomas in humans, and cause disease in a tissue-specific fashion. Only a single copy of the gene has been found to be mutated in tumours. Mutations of R132 to H, C, S, G, V, or L have been reported to be neomorphic, abolishing the conversion of isocitrate to alpha-KG. Instead, alpha-KG is converted to R(-)-2-hydroxyglutarate (2HG). Elevated levels of 2HG correlate with an elevated risk of malignant brain tumors. Neomorphic mutations in IDH1 and IDH2 convert alpha-KG to 2-hydroxyglutarate (2-HG) occur in a high percentage of patients with cytogenetically normal acute myeloid leukemia (AML).

Protein type: EC 1.1.1.42; Carbohydrate Metabolism - citrate (TCA) cycle; Other Amino Acids Metabolism - glutathione; Oxidoreductase

Chromosomal Location of Human Ortholog: 2q33.3

Cellular Component: cytoplasm; cytosol; peroxisomal matrix; peroxisome

Molecular Function: (R)-2-hydroxyglutarate dehydrogenase activity; isocitrate dehydrogenase (NADP+) activity; magnesium ion binding; protein homodimerization activity; receptor binding

Biological Process: 2-oxoglutarate metabolic process; isocitrate metabolic process; NADPH regeneration

Disease: Glioma Susceptibility 1
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Product References and Citations for IDH1 antibody

   Geisbrecht, B.V. and Gould, S.J. 1999. The human PICD gene encodes a cytoplasmic and peroxisomal NADP+-dependent isocitrate dehydrogenase. J. Biol. Chem. 274: 30527-30533
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 Research Articles on IDH1    1. Most IDH mutant gliomas with TP53 mutations had at least one of the CNAs +7q, +8q, -9p, and -11p.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IDH1 antibody Pathways associated with IDH1 antibody
 Reference Product  PubMed Publications
 MDH1 antibody  >10 publications with IDH1 and MDH1
 ACO1 antibody  >8 publications with IDH1 and ACO1
 ACLY antibody  >5 publications with IDH1 and ACLY
 GOT1 antibody  >2 publications with IDH1 and GOT1
 ACO2 antibody  >2 publications with IDH1 and ACO2
 GLUD1 antibody  >1 publications with IDH1 and GLUD1
 GOT2 antibody  >1 publications with IDH1 and GOT2
 IDH3B antibody  >1 publications with IDH1 and IDH3B
 IDH3A antibody  >1 publications with IDH1 and IDH3A
 Products by Pathway  Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 Abnormal Conversion Of 2-oxoglutarate To 2-hydroxyglutarate Pathway antibodies  Abnormal Conversion Of 2-oxoglutarate To 2-hydroxyglutarate Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
Diseases associated with IDH1 antibody Organs/Tissues associated with IDH1 antibody
 Disease Name  Pubmed Publications
 Glioma Antibodies  >883 publications with IDH1 and Glioma
 Nervous System Diseases Antibodies  >672 publications with IDH1 and Nervous System Diseases
 Leukemia, Myeloid, Acute Antibodies  >288 publications with IDH1 and Leukemia, Myeloid, Acute
 Oligodendroglioma Antibodies  >174 publications with IDH1 and Oligodendroglioma
 Cell Transformation, Neoplastic Antibodies  >58 publications with IDH1 and Cell Transformation, Neoplastic
 Cholangiocarcinoma Antibodies  >39 publications with IDH1 and Cholangiocarcinoma
 Neoplasms, Experimental Antibodies  >38 publications with IDH1 and Neoplasms, Experimental
 Neoplasm Recurrence, Local Antibodies  >37 publications with IDH1 and Neoplasm Recurrence, Local
 Necrosis Antibodies  >37 publications with IDH1 and Necrosis
 Liver Neoplasms Antibodies  >22 publications with IDH1 and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >774 publications with IDH1 and Brain
 Blood Antibodies  >149 publications with IDH1 and Blood
 Bone Antibodies  >96 publications with IDH1 and Bone
 Bone Marrow Antibodies  >54 publications with IDH1 and Bone Marrow
 Liver Antibodies  >44 publications with IDH1 and Liver
 Vascular Antibodies  >37 publications with IDH1 and Vascular
 Nerve Antibodies  >32 publications with IDH1 and Nerve
 Lung Antibodies  >20 publications with IDH1 and Lung
 Skin Antibodies  >12 publications with IDH1 and Skin
 Prostate Antibodies  >12 publications with IDH1 and Prostate
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