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anti-IR antibody :: IR Antibody

Scan QR to view Datasheet Catalog #    MBS852561
Western Blot (WB)
Unit / Price
0.1 mg  /  $215 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IR, Antibody

★Popular Item★
 Also Known As   

IR (Phospho-Thr1375) Antibody

 Product Synonym Names    CD220 antigen; EC 2.7.10.1; insulin receptor; IR; kinase InsR
 Product Gene Name   

anti-IR antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    125853
 3D Structure    ModBase 3D Structure for P06213
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 Species Reactivity    Human, Mouse, Rat
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 Specificity    INSR (Phospho-Thr1375) antibody detects endogenous levels of INSR only when phosphorylated at threonine 1375.
 Purity/Purification    Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
 Form/Format    In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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 Immunogen    The antiserum was produced against synthesized phosphopeptide derived from human INSR around the phosphorylation site of threonine 1375 (I-L-TP-L-P).
 All Sites    Human: Thr1375
Mouse: Thr1365
Rat: Thr1376
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 Preparation and Storage    Store at-20 degree C for 1 year.
 Other Notes    Small volumes of anti-IR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-IR antibody    Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
 Applications Tested/Suitable for anti-IR antibody   

Western Blot (WB), ELISA (EIA)

 Application Notes for anti-IR antibody    WB: 1:500~1:1000
ELISA: 1:5000
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 Western Blot (WB) of anti-IR antibody    Western blot analysis of extracts from HepG2 cells, using INSR (Phospho-Thr1375) antibody.
anti-IR antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for IR. It may not necessarily be applicable to this product.
 NCBI GI #    119395736
 NCBI GeneID    3643
 NCBI Accession #    NP_000199.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000208.3 [Other Products]
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 UniProt Primary Accession #    P06213 [Other Products]
 UniProt Secondary Accession #    Q17RW0; Q59H98; Q9UCB7; Q9UCB8; Q9UCB9 [Other Products]
 UniProt Related Accession #    P06213 [Other Products]
 Molecular Weight    155,146 Da
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 NCBI Official Full Name    insulin receptor isoform Long preproprotein
 NCBI Official Synonym Full Names    insulin receptor
 NCBI Official Symbol    INSR [Similar Products]
 NCBI Official Synonym Symbols   
HHF5; CD220
[Similar Products]
 NCBI Protein Information    insulin receptor
 UniProt Protein Name    Insulin receptor
 UniProt Synonym Protein Names   
CD_antigen: CD220
 Protein Family    Irditoxin
 UniProt Gene Name    INSR [Similar Products]
 UniProt Synonym Gene Names    IR [Similar Products]
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 NCBI Summary for IR    This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
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 UniProt Comments for IR    Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosine residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. Binding of the SH2 domains of PI3K to phosphotyrosines on IRS1 leads to the activation of PI3K and the generation of phosphatidylinositol-(3, 4, 5)-triphosphate (PIP3), a lipid second messenger, which activates several PIP3-dependent serine/threonine kinases, such as PDPK1 and subsequently AKT/PKB. The net effect of this pathway is to produce a translocation of the glucose transporter SLC2A4/GLUT4 from cytoplasmic vesicles to the cell membrane to facilitate glucose transport. Moreover, upon insulin stimulation, activated AKT/PKB is responsible for: anti-apoptotic effect of insulin by inducing phosphorylation of BAD; regulates the expression of gluconeogenic and lipogenic enzymes by controlling the activity of the winged helix or forkhead (FOX) class of transcription factors. Another pathway regulated by PI3K-AKT/PKB activation is mTORC1 signaling pathway which regulates cell growth and metabolism and integrates signals from insulin. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 thereby activating mTORC1 pathway. The Ras/RAF/MAP2K/MAPK pathway is mainly involved in mediating cell growth, survival and cellular differentiation of insulin. Phosphorylated IRS1 recruits GRB2/SOS complex, which triggers the activation of the Ras/RAF/MAP2K/MAPK pathway. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
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Product References and Citations for anti-IR antibody

   Ebina Y., Cell 40:747-758(1985). Ullrich A., Nature 313:756-761(1985). Seino S., Diabetes 39:123-128(1990).
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 Research Articles on IR    1. Findings demonstrate that, in human breast cancer cells, DDR1 regulates IR expression and ligand dependent biological actions. This novel functional crosstalk is likely clinically relevant.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-IR antibodyPathways associated with anti-IR antibody
 Reference Product  PubMed Publications
 IGF1R antibody  >65 publications with IR and IGF1R
 IRS1 antibody  >37 publications with IR and IRS1
 INS antibody  >25 publications with IR and INS
 IGF2 antibody  >19 publications with IR and IGF2
 IRS2 antibody  >17 publications with IR and IRS2
 SRC antibody  >8 publications with IR and SRC
 PIK3R1 antibody  >6 publications with IR and PIK3R1
 SOCS3 antibody  >4 publications with IR and SOCS3
 PTPN1 antibody  >4 publications with IR and PTPN1
 MAPK3 antibody  >4 publications with IR and MAPK3
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 IRS Activation Pathway antibodies  IRS Activation Pathway Diagram
 IRS-related Events Pathway antibodies  IRS-related Events Pathway Diagram
Diseases associated with anti-IR antibodyOrgans/Tissues associated with anti-IR antibody
 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >126 publications with IR and Insulin Resistance
 Hyperinsulinism Antibodies  >84 publications with IR and Hyperinsulinism
 Neoplasms Antibodies  >78 publications with IR and Neoplasms
 Hyperglycemia Antibodies  >22 publications with IR and Hyperglycemia
 Hypertension Antibodies  >17 publications with IR and Hypertension
 Diabetes Mellitus, Experimental Antibodies  >14 publications with IR and Diabetes Mellitus, Experimental
 Breast Neoplasms Antibodies  >14 publications with IR and Breast Neoplasms
 Atherosclerosis Antibodies  >12 publications with IR and Atherosclerosis
 Brain Diseases Antibodies  >12 publications with IR and Brain Diseases
 Inflammation Antibodies  >10 publications with IR and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >97 publications with IR and Blood
 Muscle Antibodies  >50 publications with IR and Muscle
 Liver Antibodies  >50 publications with IR and Liver
 Brain Antibodies  >44 publications with IR and Brain
 Ovary Antibodies  >30 publications with IR and Ovary
 Adipose Tissue Antibodies  >21 publications with IR and Adipose Tissue
 Pancreas Antibodies  >18 publications with IR and Pancreas
 Connective Tissue Antibodies  >15 publications with IR and Connective Tissue
 Vascular Antibodies  >13 publications with IR and Vascular
 Bone Antibodies  >10 publications with IR and Bone
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