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anti-IRS-1 (Paired S312) antibody :: Rabbit IRS-1 (Paired S312) Antibody

Scan QR to view Datasheet Catalog #    MBS462058
Testing Data
Unit / Price
0.1 mg  /  $285 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IRS-1 (Paired S312) (IRS-1 (Paired S312)), Antibody

 Also Known As   

Rabbit anti IRS-1 (Paired 312) antibody

 Product Synonym Names    Rabbit anti-IRS-1 (Paired S312); Anti-IRS-1 (Paired312); Insulin-receptor substrate 1 (IRS-1).
 Product Gene Name   

anti-IRS-1 (Paired S312) antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 2; NC_000002.11 (227596033..227663506, complement). Location: 2q36
 OMIM    125853
 3D Structure    ModBase 3D Structure for P35568
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 Host    Rabbit
 Species Reactivity    Human, Rat, Mouse
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 Specificity    This antibody recognizes ~165 kDa of human IRS-1 (without phosphorylation at Ser312). It also reacts with mouse and rat. The other species are not tested.
 Purity/Purification    The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
 Form/Format    This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
 Concentration    Size: 100 ug/200 ul (lot specific)
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 Antigen Preparation    A synthetic peptide corresponding to the epitope ATSPA without phosphorylation of human IRS-1.
 Positive Control    CHO cell lysate
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 Preparation and Storage    The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
 Other Notes    Small volumes of anti-IRS-1 (Paired S312) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-IRS-1 (Paired S312) antibody

   Insulin Receptor Substrate-1 (IRS-1), 165 kDa cytoplasmic docking protein, is one of the major endogenous substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain containing proteins, which mediate the metabolic and growth promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser312 of IRS-1 is phosphorylated by JNK and IKK and Ser789 is phosphorylated by SIK-2, a member of AMPK family. The phosphorylation of Tyr612 and Ser636/639 is mediated by the PKC and mTOR pathways, respectively and phosphorylation at Ser1101 is mediated by PKC, resulting in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.
 Applications Tested/Suitable for anti-IRS-1 (Paired S312) antibody   

ELISA, Western Blot, Immunohistochemistry

 Application Notes for anti-IRS-1 (Paired S312) antibody    Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 0.5-2 ug/ml
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 Testing Data of anti-IRS-1 (Paired S312) antibody    anti-IRS-1 (Paired S312) antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for IRS-1 (Paired S312). It may not necessarily be applicable to this product.
 NCBI GI #    547738
 NCBI GeneID    3667
 NCBI Accession #    P35568.1 [Other Products]
 NCBI GenBank Nucleotide #    P35568 [Other Products]
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 UniProt Primary Accession #    P35568 [Other Products]
 UniProt Related Accession #    P35568 [Other Products]
 Molecular Weight    0.5-2 ug/ml
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 NCBI Official Full Name    Insulin receptor substrate 1
 NCBI Official Synonym Full Names    insulin receptor substrate 1
 NCBI Official Symbol    IRS1 [Similar Products]
 NCBI Official Synonym Symbols   
HIRS-1
[Similar Products]
 NCBI Protein Information    insulin receptor substrate 1; IRS-1; OTTHUMP00000164234
 UniProt Protein Name    Insulin receptor substrate 1
 UniProt Gene Name    IRS1 [Similar Products]
 UniProt Entry Name    IRS1_HUMAN
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 NCBI Summary for IRS-1 (Paired S312)    This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq]
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 UniProt Comments for IRS-1 (Paired S312)    Function: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit

By similarity. Ref.28

Subunit structure: Interacts with UBTF and PIK3CA

By similarity. Interacts (via phosphorylated YXXM motifs) with PIK3R1

By similarity. Interacts with ROCK1

By similarity. Interacts (via PH domain) with PHIP

By similarity. Interacts with GRB2

By similarity. Interacts with SOCS7. Interacts (via IRS-type PTB domain) with IGF1R and INSR (via the tyrosine-phosphorylated NPXY motif). Ref.5 Ref.6 Ref.7 Ref.9

Post-translational modification: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor

By similarity.Phosphorylation of Tyr-896 is required for GRB2-binding

Polymorphism: The Arg-971 polymorphism impairs the ability of insulin to stimulate glucose transport, glucose transporter translocation, and glycogen synthesis by affecting the PI3K/AKT1/GSK3 signaling pathway. The polymorphism at Arg-971 may contribute to the in vivo insulin resistance observed in carriers of this variant. Arg-971 could contribute to the risk for atherosclerotic cardiovascular diseases associated with non-insulin-dependent diabetes mellitus (NIDDM) by producing a cluster of insulin resistance-related metabolic abnormalities. In insulin-stimulated human endothelial cells from carriers of the Arg-971 polymorphism, genetic impairment of the IRS1/PI3K/PDPK1/AKT1 insulin signaling cascade results in impaired insulin-stimulated nitric oxide (NO) release and suggested that this may be a mechanism through which the Arg-971 polymorphism contributes to the genetic predisposition to develop endothelial dysfunction and cardiovascular disease. The Arg-971 polymorphism not only reduces phosphorylation of the substrate but allows IRS1 to act as an inhibitor of PI3K, producing global insulin resistance.

Involvement in disease: Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [

MIM:125853]. Ref.22 Ref.23 Ref.25 Ref.28 Ref.29

Sequence similarities: Contains 1 IRS-type PTB domain.Contains 1 PH domain.
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Product References and Citations for anti-IRS-1 (Paired S312) antibody

   Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307.
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 Research Articles on IRS-1 (Paired S312)    1. miR-126 was actively involved in the development of insulin resistance induced by mitochondrial dysfunction.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-IRS-1 (Paired S312) antibodyPathways associated with anti-IRS-1 (Paired S312) antibody
 Reference Product  PubMed Publications
 IGF1R antibody  >212 publications with IRS-1 (Paired S312) and IGF1R
 AKT1 antibody  >165 publications with IRS-1 (Paired S312) and AKT1
 GRB2 antibody  >136 publications with IRS-1 (Paired S312) and GRB2
 MTOR antibody  >107 publications with IRS-1 (Paired S312) and MTOR
 MAPK1 antibody  >73 publications with IRS-1 (Paired S312) and MAPK1
 SHC1 antibody  >64 publications with IRS-1 (Paired S312) and SHC1
 JAK2 antibody  >55 publications with IRS-1 (Paired S312) and JAK2
 PTPN11 antibody  >41 publications with IRS-1 (Paired S312) and PTPN11
 INSR antibody  >32 publications with IRS-1 (Paired S312) and INSR
 SOCS3 antibody  >30 publications with IRS-1 (Paired S312) and SOCS3
 Products by Pathway  Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 EPO Receptor Signaling Pathway antibodies  EPO Receptor Signaling Pathway Diagram
 IGF1 Pathway antibodies  IGF1 Pathway Diagram
 IL-2 Signaling Pathway antibodies  IL-2 Signaling Pathway Diagram
Diseases associated with anti-IRS-1 (Paired S312) antibodyOrgans/Tissues associated with anti-IRS-1 (Paired S312) antibody
 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >920 publications with IRS-1 (Paired S312) and Insulin Resistance
 Hyperinsulinism Antibodies  >622 publications with IRS-1 (Paired S312) and Hyperinsulinism
 Neoplasms Antibodies  >310 publications with IRS-1 (Paired S312) and Neoplasms
 Obesity Antibodies  >286 publications with IRS-1 (Paired S312) and Obesity
 Diabetes Mellitus, Type 2 Antibodies  >254 publications with IRS-1 (Paired S312) and Diabetes Mellitus, Type 2
 Necrosis Antibodies  >132 publications with IRS-1 (Paired S312) and Necrosis
 Breast Neoplasms Antibodies  >78 publications with IRS-1 (Paired S312) and Breast Neoplasms
 Inflammation Antibodies  >64 publications with IRS-1 (Paired S312) and Inflammation
 Hypertension Antibodies  >64 publications with IRS-1 (Paired S312) and Hypertension
 Fatty Liver Antibodies  >63 publications with IRS-1 (Paired S312) and Fatty Liver
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >698 publications with IRS-1 (Paired S312) and Muscle
 Liver Antibodies  >362 publications with IRS-1 (Paired S312) and Liver
 Connective Tissue Antibodies  >200 publications with IRS-1 (Paired S312) and Connective Tissue
 Pancreas Antibodies  >124 publications with IRS-1 (Paired S312) and Pancreas
 Ovary Antibodies  >98 publications with IRS-1 (Paired S312) and Ovary
 Brain Antibodies  >84 publications with IRS-1 (Paired S312) and Brain
 Heart Antibodies  >83 publications with IRS-1 (Paired S312) and Heart
 Kidney Antibodies  >82 publications with IRS-1 (Paired S312) and Kidney
 Nerve Antibodies  >37 publications with IRS-1 (Paired S312) and Nerve
 Bone Antibodies  >33 publications with IRS-1 (Paired S312) and Bone
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