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anti-ITCH antibody :: anti-Human, Mouse ITCH Antibody

Scan QR to view Datasheet Catalog #    MBS851134
Unit / Price
0.1 mg  /  $215 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ITCH, Antibody

 Also Known As   

ITCH (Phospho-Tyr420) Antibody

 Product Synonym Names    AIF4; AIP4; atrophin-1-interacting protein 4; EC 6.3.2.-; Itchy homolog E3 ubiquitin protein ligase; NAPP1; NFE2-associated polypeptide 1; ubiquitin protein ligase ITCH
 Product Gene Name   

anti-ITCH antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    606409
 3D Structure    ModBase 3D Structure for Q96J02
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 Species Reactivity    Human, Mouse
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 Specificity    ITCH (Phospho-Tyr420) antibody detects endogenous levels of ITCH only when phosphorylated at tyrosine 420.
 Purity/Purification    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
 Form/Format    In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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 Immunogen    The antiserum was produced against synthesized phosphopeptide derived from human ITCH around the phosphorylation site of tyrosine 420 (F-I-YP-G-N).
 All Sites    Human: Tyr420
Mouse: Tyr381
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 Preparation and Storage    Store at-20 degree C for 1 year.
 Other Notes    Small volumes of anti-ITCH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-ITCH antibody    Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
 Applications Tested/Suitable for anti-ITCH antibody   

Immunohistochemistry (IHC), ELISA (EIA)

 Application Notes for anti-ITCH antibody    IHC: 1:50-1:100
ELISA: 1:20000
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 Testing Data of anti-ITCH antibody    P-peptide-+
anti-ITCH antibody Testing Data image
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 Immunohistochemistry (IHC) of anti-ITCH antibody    Immunohistochemistry analysis of paraffin-embedded human brain tissue using ITCH (Phospho-Tyr420) antibody. ITCH (Phospho-Tyr420) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.
anti-ITCH antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for ITCH. It may not necessarily be applicable to this product.
 NCBI GI #    380420335
 NCBI GeneID    83737
 NCBI Accession #    NP_001244066.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001257137.2 [Other Products]
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 UniProt Primary Accession #    Q96J02 [Other Products]
 UniProt Secondary Accession #    O43584; Q5QP37; Q5TEL0; Q96F66; Q9BY75; Q9H451; Q9H4U5; A6NEW4; B4E234; E1P5P3; F5H217 [Other Products]
 UniProt Related Accession #    Q96J02 [Other Products]
 Molecular Weight    86,498 Da
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 NCBI Official Full Name    E3 ubiquitin-protein ligase Itchy homolog isoform 1
 NCBI Official Synonym Full Names    itchy E3 ubiquitin protein ligase
 NCBI Official Symbol    ITCH [Similar Products]
 NCBI Official Synonym Symbols   
AIF4; AIP4; ADMFD; NAPP1
[Similar Products]
 NCBI Protein Information    E3 ubiquitin-protein ligase Itchy homolog
 UniProt Protein Name    E3 ubiquitin-protein ligase Itchy homolog
 UniProt Synonym Protein Names   
Atrophin-1-interacting protein 4; AIP4; HECT-type E3 ubiquitin transferase Itchy homolog; NFE2-associated polypeptide 1; NAPP1
 Protein Family    E3 ubiquitin-protein ligase
 UniProt Gene Name    ITCH [Similar Products]
 UniProt Synonym Gene Names    Itch; AIP4; NAPP1 [Similar Products]
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 NCBI Summary for ITCH    This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
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 UniProt Comments for ITCH    Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:14602072, PubMed:17028573, PubMed:16387660, PubMed:18718448, PubMed:18718449, PubMed:11046148, PubMed:19592251, PubMed:19116316, PubMed:19881509, PubMed:20491914, PubMed:20392206, PubMed:20068034, PubMed:23146885, PubMed:24790097, PubMed:25631046). Catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation (PubMed:17028573, PubMed:18718448, PubMed:19131965, PubMed:19881509). Involved in the control of inflammatory signaling pathways (PubMed:19131965). Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways (PubMed:19131965). Promotes the association of the complex after TNF stimulation (PubMed:19131965). Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains (PubMed:19131965). This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1 (PubMed:19131965). Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways (PubMed:19592251). Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response (PubMed:18718448, PubMed:20491914). Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages (PubMed:18718448). Mediates JUN ubiquitination and degradation (). Mediates JUNB ubiquitination and degradation (PubMed:16387660). Critical regulator of type 2 helper T (Th2) cell cytokine production by inducing JUNB ubiquitination and degradation (). Involved in the negative regulation of MAVS-dependent cellular antiviral responses (PubMed:19881509). Ubiquitinates MAVS through 'Lys-48'-linked conjugation resulting in MAVS proteasomal degradation (PubMed:19881509). Following ligand stimulation, regulates sorting of Wnt receptor FZD4 to the degradative endocytic pathway probably by modulating PI42KA activity (PubMed:23146885). Ubiquitinates PI4K2A and negatively regulates its catalytic activity (PubMed:23146885). Ubiquitinates chemokine receptor CXCR4 and regulates sorting of CXCR4 to the degradative endocytic pathway following ligand stimulation by ubiquitinating endosomal sorting complex required for transport ESCRT-0 components HGS and STAM (PubMed:14602072, PubMed:23146885). Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through 'Lys-29'-linked polyubiquitination (PubMed:17028573, PubMed:18628966, PubMed:23886940). Ubiquitinates SNX9 (PubMed:20491914). Ubiquitinates MAP3K7 through 'Lys-48'-linked conjugation (). Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP (PubMed:20068034). Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteasomal degradation of p15 BID (PubMed:20392206). Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046).
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Product References and Citations for anti-ITCH antibody

   Chen X., Genomics 73:238-241(2001). Deloukas P., Nature 414:865-871(2001). Wood J.D., Mol. Cell. Neurosci. 11:149-160(1998).
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 Research Articles on ITCH    1. Mechanistically, YOD1 deubiquitinates ITCH, an E3 ligase of LATS, and enhances the stability of ITCH, which leads to reduced levels of LATS and a subsequent increase in the YAP/TAZ level.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ITCH antibodyPathways associated with anti-ITCH antibody
 Reference Product  PubMed Publications
 JUN antibody  >1555 publications with ITCH and JUN
 TRPV1 antibody  >50 publications with ITCH and TRPV1
 CBL antibody  >21 publications with ITCH and CBL
 NUMB antibody  >11 publications with ITCH and NUMB
 NOTCH1 antibody  >10 publications with ITCH and NOTCH1
 RNF11 antibody  >9 publications with ITCH and RNF11
 OCLN antibody  >4 publications with ITCH and OCLN
 TRPV4 antibody  >4 publications with ITCH and TRPV4
 NDFIP2 antibody  >3 publications with ITCH and NDFIP2
 TRPC4 antibody  >1 publications with ITCH and TRPC4
 Products by Pathway  Pathway Diagram
 Activated NOTCH1 Transmits Signal To The Nucleus Pathway antibodies  Activated NOTCH1 Transmits Signal To The Nucleus Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downregulation Of ERBB4 Signaling Pathway antibodies  Downregulation Of ERBB4 Signaling Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
Diseases associated with anti-ITCH antibodyOrgans/Tissues associated with anti-ITCH antibody
 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >4203 publications with ITCH and Immune System Diseases
 Neoplasms Antibodies  >1799 publications with ITCH and Neoplasms
 Nervous System Diseases Antibodies  >1540 publications with ITCH and Nervous System Diseases
 Liver Diseases Antibodies  >1383 publications with ITCH and Liver Diseases
 Poisoning Antibodies  >794 publications with ITCH and Poisoning
 Inflammation Antibodies  >751 publications with ITCH and Inflammation
 Drug Toxicity Antibodies  >746 publications with ITCH and Drug Toxicity
 Kidney Diseases Antibodies  >678 publications with ITCH and Kidney Diseases
 Edema Antibodies  >593 publications with ITCH and Edema
 Skin Neoplasms Antibodies  >531 publications with ITCH and Skin Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >5873 publications with ITCH and Skin
 Blood Antibodies  >2687 publications with ITCH and Blood
 Liver Antibodies  >1600 publications with ITCH and Liver
 Kidney Antibodies  >539 publications with ITCH and Kidney
 Bone Antibodies  >453 publications with ITCH and Bone
 Vascular Antibodies  >382 publications with ITCH and Vascular
 Brain Antibodies  >301 publications with ITCH and Brain
 Heart Antibodies  >300 publications with ITCH and Heart
 Eye Antibodies  >268 publications with ITCH and Eye
 Muscle Antibodies  >242 publications with ITCH and Muscle
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