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Mouse IgM Isotype Control Antibody

Scan QR to view Datasheet Catalog #    MBS8501295
Flow Cytometry
Unit / Price
50 Tests  /  $225 +1 FREE 8GB USB
100 Tests  /  $310 +1 FREE 8GB USB
 
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 Product Name   

IgM Isotype Control, Antibody

★Popular Item★
 Also Known As   

Mouse IgM Isotype Control, PE-Cy5

 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Isotype    Mouse IgGM
 Clone Number    4A7C2C7C12
 Host    Mouse
 Species Reactivity    Human,Not yet tested in other species.
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 Form/Format    Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
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 Experimental Methods    1.Take 100ul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10ul labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3;
[Cell fixation: 2% formaldehyde solution]
 Notices    1.This reagent has been pre-diluted for use at the recommended Volume per Test.
We typically use 1 X 106 cells in a 100ul experimental sample (a test);
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
4. If the sample cannot be timely analysis, please fixed;
5. For research use only, not for diagnostic or therapeutic use.
 Related Product    MBS8506029 Mouse IgM Isotype Control,Purified mAb FCM IF
MBS8501575 Mouse IgM Isotype Control,FITC Conjugated mAb FCM IF
MBS8502565 Mouse IgM Isotype Control,PE Conjugated mAb FCM IF
MBS8501295 Mouse IgM Isotype Control,PE-Cy5 Conjugated mAb FCM IF
MBS8506051 Mouse IgM Isotype Control,APC Conjugated mAb FCM IF
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 Preparation and Storage    Store at 4 degree C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
 Other Notes    Small volumes of anti-IgM Isotype Control antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-IgM Isotype Control antibody

   This antibody can be used as a mouse IgM isotype control in flow cytometry and other applications.
 Product Categories/Family for anti-IgM Isotype Control antibody    Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody
 Applications Tested/Suitable for anti-IgM Isotype Control antibody   

Flow Cytometry (FC/FACS), Immunofluorescence (IF)

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 Flow Cytometry of anti-IgM Isotype Control antibody    anti-IgM Isotype Control antibody Flow Cytometry (FC/FACS) image
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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