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anti-MAPKK5 (MEK5) (Paired S311/T315) antibody :: Rabbit MAPKK5 (MEK5) (Paired S311/T315) Antibody

Scan QR to view Datasheet Catalog #    MBS462076
Testing Data
Unit / Price
0.1 mg  /  $285 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MAPKK5 (MEK5) (Paired S311/T315) (MAPKK5 (MEK5) (Paired S311/T315)), Antibody

 Also Known As   

Rabbit anti MEK5 (Non-Phospho-specific)

 Product Synonym Names    Rabbit anti-MEK5 (Nonphospho); MAPKK5; MEK5; MKK5; MAPK/ERK Kinase 5; SKK5.
 Product Gene Name   

anti-MAPKK5 (MEK5) (Paired S311/T315) antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 15; NC_000015.9 (67835021..68099452). Location: 15q23
 OMIM    602520
 3D Structure    ModBase 3D Structure for Q13163
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 Host    Rabbit
 Species Reactivity    Human, Rat, Mouse, Chicken, Canine
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 Specificity    This antibody recognizes ~49 kDa of human MEK5 protein without phosphorylation sites of Ser 311/Thr315. It does not cross react to phospho MEK5. This antibody reacts with mouse and rat, chicken. The other species are not tested.
 Purity/Purification    The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
 Form/Format    This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
 Concentration    Size: 100 ug/200 ul (lot specific)
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 Antigen Preparation    A synthetic peptide surrounding the epitope -SIAKT- without any phosphorylation modification. This sequence is identical among human, rat, mouse, chicken, and dog.
 Positive Control    Mouse liver
 Cellular Location    Cytoplasmic
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 Preparation and Storage    The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
 Other Notes    Small volumes of anti-MAPKK5 (MEK5) (Paired S311/T315) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-MAPKK5 (MEK5) (Paired S311/T315) antibody

   The MEK5 (also known as MKK5, MAPKK5, SKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. It catalyzes tyrosine phosphorylation of ERK5 whereas MEK5 itself is regulated by MEKK3. An important link between MEK5 and metastatic prostrate cancer has been demonstrated. Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation.
 Applications Tested/Suitable for anti-MAPKK5 (MEK5) (Paired S311/T315) antibody   

ELISA, Western Blot

 Application Notes for anti-MAPKK5 (MEK5) (Paired S311/T315) antibody    Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
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 Testing Data of anti-MAPKK5 (MEK5) (Paired S311/T315) antibody    anti-MAPKK5 (MEK5) (Paired S311/T315) antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for MAPKK5 (MEK5) (Paired S311/T315). It may not necessarily be applicable to this product.
 NCBI GI #    21729895
 NCBI GeneID    5607
 NCBI Accession #    NP_660143.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_145160.1 [Other Products]
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 UniProt Primary Accession #    Q13163 [Other Products]
 UniProt Secondary Accession #    Q92961; Q92962 [Other Products]
 UniProt Related Accession #    Q13163 [Other Products]
 Molecular Weight    49 kDa
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 NCBI Official Full Name    dual specificity mitogen-activated protein kinase kinase 5 isoform A
 NCBI Official Synonym Full Names    mitogen-activated protein kinase kinase 5
 NCBI Official Symbol    MAP2K5 [Similar Products]
 NCBI Official Synonym Symbols   
MEK5; MAPKK5; PRKMK5; HsT17454
[Similar Products]
 NCBI Protein Information    dual specificity mitogen-activated protein kinase kinase 5; MEK 5; MAPKK 5; MAPK/ERK kinase 5; OTTHUMP00000164363; MAP kinase kinase 5; MAP kinase kinase MEK5b
 UniProt Protein Name    Dual specificity mitogen-activated protein kinase kinase 5
 UniProt Synonym Protein Names   
MAPK/ERK kinase 5
 UniProt Gene Name    MAP2K5 [Similar Products]
 UniProt Synonym Gene Names    MEK5; MKK5; PRKMK5 [Similar Products]
 UniProt Entry Name    MP2K5_HUMAN
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 NCBI Summary for MAPKK5 (MEK5) (Paired S311/T315)    The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq]
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 UniProt Comments for MAPKK5 (MEK5) (Paired S311/T315)    Function: Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis. Ref.1 Ref.2

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Subunit structure: Interacts with PARD6A, MAP3K3 and MAPK7. Forms a complex with SQSTM1 and PRKCZ or PRKCI

By similarity. Interacts with Yersinia yopJ. Ref.1 Ref.7

Tissue specificity: Expressed in many adult tissues. Abundant in heart and skeletal muscle.

Domain: Binds MAP3K2/MAP3K3 and MAPK7 via non-overlapping residues of the OPR domain. This domain also mediates interactions with SQSTM1 and PARD6A

Post-translational modification: Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases

By similarity.Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Contains 1 OPR domain.Contains 1 protein kinase domain.
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Product References and Citations for anti-MAPKK5 (MEK5) (Paired S311/T315) antibody

   Tetsuro Shishido, et al. Effects of MEK5/ERK5 Association on Small Ubiquitin-Related Modification of ERK5: Implications for Diabetic Ventricular Dysfunction After Myocardial Infarction. Circulation Research. 2008; 102:1416.
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 Research Articles on MAPKK5 (MEK5) (Paired S311/T315)    1. MEK5 activation by laminar shear stress inhibits inflammatory responses in microvascular endothelial cells, in part through ERK5-dependent induction of KLF4
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MAPKK5 (MEK5) (Paired S311/T315) antibodyPathways associated with anti-MAPKK5 (MEK5) (Paired S311/T315) antibody
 Reference Product  PubMed Publications
 MAP3K3 antibody  >3 publications with MAPKK5 (MEK5) (Paired S311/T315) and MAP3K3
 MAP3K2 antibody  >2 publications with MAPKK5 (MEK5) (Paired S311/T315) and MAP3K2
 SQSTM1 antibody  >1 publications with MAPKK5 (MEK5) (Paired S311/T315) and SQSTM1
 PRKCZ antibody  >1 publications with MAPKK5 (MEK5) (Paired S311/T315) and PRKCZ
 Products by Pathway  Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 ErbB1 Downstream Signaling Pathway antibodies  ErbB1 Downstream Signaling Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Integrin-mediated Cell Adhesion Pathway antibodies  Integrin-mediated Cell Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
Diseases associated with anti-MAPKK5 (MEK5) (Paired S311/T315) antibodyOrgans/Tissues associated with anti-MAPKK5 (MEK5) (Paired S311/T315) antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >16 publications with MAPKK5 (MEK5) (Paired S311/T315) and Neoplasms
 Mental Disorders Antibodies  >9 publications with MAPKK5 (MEK5) (Paired S311/T315) and Mental Disorders
 Prostatic Neoplasms Antibodies  >5 publications with MAPKK5 (MEK5) (Paired S311/T315) and Prostatic Neoplasms
 Carcinoma Antibodies  >5 publications with MAPKK5 (MEK5) (Paired S311/T315) and Carcinoma
 Cardiovascular Diseases Antibodies  >4 publications with MAPKK5 (MEK5) (Paired S311/T315) and Cardiovascular Diseases
 Kidney Diseases Antibodies  >3 publications with MAPKK5 (MEK5) (Paired S311/T315) and Kidney Diseases
 Cardiomegaly Antibodies  >3 publications with MAPKK5 (MEK5) (Paired S311/T315) and Cardiomegaly
 Neoplasm Metastasis Antibodies  >3 publications with MAPKK5 (MEK5) (Paired S311/T315) and Neoplasm Metastasis
 Skin Diseases Antibodies  >3 publications with MAPKK5 (MEK5) (Paired S311/T315) and Skin Diseases
 Inflammation Antibodies  >3 publications with MAPKK5 (MEK5) (Paired S311/T315) and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >9 publications with MAPKK5 (MEK5) (Paired S311/T315) and Muscle
 Brain Antibodies  >9 publications with MAPKK5 (MEK5) (Paired S311/T315) and Brain
 Vascular Antibodies  >6 publications with MAPKK5 (MEK5) (Paired S311/T315) and Vascular
 Prostate Antibodies  >6 publications with MAPKK5 (MEK5) (Paired S311/T315) and Prostate
 Blood Antibodies  >5 publications with MAPKK5 (MEK5) (Paired S311/T315) and Blood
 Kidney Antibodies  >4 publications with MAPKK5 (MEK5) (Paired S311/T315) and Kidney
 Nerve Antibodies  >4 publications with MAPKK5 (MEK5) (Paired S311/T315) and Nerve
 Heart Antibodies  >4 publications with MAPKK5 (MEK5) (Paired S311/T315) and Heart
 Embryonic Tissue Antibodies  >2 publications with MAPKK5 (MEK5) (Paired S311/T315) and Embryonic Tissue
 Skin Antibodies  >2 publications with MAPKK5 (MEK5) (Paired S311/T315) and Skin
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