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anti-MDM2 antibody :: anti-Human, Rat MDM2 Antibody

Scan QR to view Datasheet Catalog #    MBS854147
Unit / Price
0.1 mg  /  $215 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MDM2, Antibody

 Also Known As   

MDM2 Antibody

 Product Synonym Names    Keratin; type I cytoskeletal 16; Cytokeratin-16; CK-16; K16
 Product Gene Name   

anti-MDM2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    164785
 3D Structure    ModBase 3D Structure for Q00987
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 Species Reactivity    Human, Rat
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 Specificity    MDM2 antibody detects endogenous levels of total MDM2 protein.
 Purity/Purification    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
 Form/Format    In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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 Immunogen    The antiserum was produced against synthesized peptide derived from human MDM2.
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 Preparation and Storage    Store at-20 degree C for 1 year
 Other Notes    Small volumes of anti-MDM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-MDM2 antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)

 Application Notes for anti-MDM2 antibody    WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:40000
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 Immunohistochemistry (IHC) of anti-MDM2 antibody    Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2 antibody.
anti-MDM2 antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-MDM2 antibody    Western blot analysis of extracts from SKOV3 cells, using MDM2 antibody.
anti-MDM2 antibody Western Blot (WB) (WB) image
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 Immunofluorescence (IF) of anti-MDM2 antibody    Peptide-+ Immunofluorescence analysis of A549 cells, using MDM2 antibody.
anti-MDM2 antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for MDM2. It may not necessarily be applicable to this product.
 NCBI GI #    223890205
 NCBI GeneID    4193
 NCBI Accession #    NP_001138811.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001145339.2 [Other Products]
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 UniProt Primary Accession #    Q00987 [Other Products]
 UniProt Secondary Accession #    Q13226; Q13297; Q13298; Q13299; Q13300; Q13301; Q53XW0; Q71TW9; Q8WYJ1; A6NL51; A8K2S6 [Other Products]
 UniProt Related Accession #    Q00987 [Other Products]
 Molecular Weight    55,991 Da
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 NCBI Official Full Name    E3 ubiquitin-protein ligase Mdm2 isoform h
 NCBI Official Synonym Full Names    MDM2 proto-oncogene
 NCBI Official Symbol    MDM2 [Similar Products]
 NCBI Official Synonym Symbols   
HDMX; hdm2; ACTFS
[Similar Products]
 NCBI Protein Information    E3 ubiquitin-protein ligase Mdm2
 UniProt Protein Name    E3 ubiquitin-protein ligase Mdm2
 UniProt Synonym Protein Names   
Double minute 2 protein; Hdm2; Oncoprotein Mdm2; RING-type E3 ubiquitin transferase Mdm2Curated; p53-binding protein Mdm2
 Protein Family    Mdm2-binding protein
 UniProt Gene Name    MDM2 [Similar Products]
 UniProt Synonym Gene Names    Hdm2 [Similar Products]
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 NCBI Summary for MDM2    This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]
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 UniProt Comments for MDM2    E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation (PubMed:12821780, PubMed:15053880, PubMed:15195100, PubMed:15632057, PubMed:16337594, PubMed:17290220, PubMed:19098711, PubMed:19219073, PubMed:19837670, PubMed:19965871, PubMed:20173098, PubMed:20385133, PubMed:20858735, PubMed:22128911). Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells (). Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis ().
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Product References and Citations for anti-MDM2 antibody

   Jer-Yen Yang, Mol. Cell. Biol., Oct 2006; 26: 7269-7282. Lubing Gu, Blood, May 2002; 99: 3367-3375. Hongyan Zhu, J. Biol. Chem., Dec 2003; 278: 49286-49292. Tatiana Dilla, Endocrinology, Jan 2000; 141: 420.
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 Research Articles on MDM2    1. Markov models of the apo-MDM2 lid region reveal diffuse yet two-state binding dynamics and receptor poses for computational docking.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-MDM2 antibodyDiseases associated with anti-MDM2 antibody
 Products by Pathway  Pathway Diagram
 AKT Phosphorylates Targets In The Cytosol Pathway antibodies  AKT Phosphorylates Targets In The Cytosol Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Aurora A Signaling Pathway antibodies  Aurora A Signaling Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >910 publications with MDM2 and Carcinoma
 Adenocarcinoma Antibodies  >377 publications with MDM2 and Adenocarcinoma
 Breast Neoplasms Antibodies  >325 publications with MDM2 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >281 publications with MDM2 and Cell Transformation, Neoplastic
 Nervous System Diseases Antibodies  >249 publications with MDM2 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >247 publications with MDM2 and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >236 publications with MDM2 and Lung Neoplasms
 Carcinoma, Squamous Cell Antibodies  >192 publications with MDM2 and Carcinoma, Squamous Cell
 Brain Diseases Antibodies  >185 publications with MDM2 and Brain Diseases
 Liver Diseases Antibodies  >151 publications with MDM2 and Liver Diseases
Organs/Tissues associated with anti-MDM2 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >384 publications with MDM2 and Blood
 Lung Antibodies  >330 publications with MDM2 and Lung
 Bone Antibodies  >249 publications with MDM2 and Bone
 Brain Antibodies  >245 publications with MDM2 and Brain
 Liver Antibodies  >226 publications with MDM2 and Liver
 Prostate Antibodies  >156 publications with MDM2 and Prostate
 Muscle Antibodies  >156 publications with MDM2 and Muscle
 Vascular Antibodies  >149 publications with MDM2 and Vascular
 Skin Antibodies  >127 publications with MDM2 and Skin
 Kidney Antibodies  >110 publications with MDM2 and Kidney
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