Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow MDM2  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Convicilin (CVCB) Recombinant Protein
 • cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP... Antibody
 • Amiloride-sensitive sodium channel subunit alpha (SCNN1A) ELISA Kit
 • FOXE2 - FOXE1 Antibody
 • EVER1 (TMC6) Antibody
 • homeobox A3 (HOXA3) ELISA Kit
 • HSPA8 (HSPA8 ) Peptide
 • GDP-mannose 4, 6-dehydratase (GMDS) ELISA Kit
 • Activating Transcription Factor 3 (ATF3) ELISA Kit
 • UDP-N-acetylglucosamine--N- acetylmuramyl-(pentapeptide ) pyro... Recombinant Protein
 • CD45 Antibody
 • CD35, C3b (CR1) Antibody
 • b2-Microglobulin (HLA-G) Antibody
 • Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) CLIA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-MDM2 antibody :: Rabbit anti-Human, Rat MDM2 Antibody

Scan QR to view Datasheet Catalog #    MBS854147
Unit / Price
0.1 mg  /  $215 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MDM2, Antibody

 Also Known As   

MDM2 Antibody

 Product Synonym Names    Keratin; type I cytoskeletal 16; Cytokeratin-16; CK-16; K16
 Product Gene Name   

anti-MDM2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    gene 614401
 3D Structure    ModBase 3D Structure for Q00987
Section Bar
 Host    Rabbit
 Species Reactivity    Human, Rat
Section Bar
 Specificity    MDM2 antibody detects endogenous levels of total MDM2 protein.
 Purity/Purification    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
 Form/Format    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    100ug/100ul (lot specific)
Section Bar
 Immunogen    The antiserum was produced against synthesized peptide derived from human MDM2.
 Pathway    Cancer/Apoptosis
Section Bar
 Preparation and Storage    Store at -20 degree C for 1 year
 Other Notes    Small volumes of anti-MDM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 Product Categories/Family for anti-MDM2 antibody    Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody
 Applications Tested/Suitable for anti-MDM2 antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)

 Application Notes for anti-MDM2 antibody    WB: 1:500~1:1000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:40000
Section Bar
 Testing Data of anti-MDM2 antibody    Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2 antibody (#C0261).
anti-MDM2 antibody Testing Data image
Section Bar
 Testing Data #2 of anti-MDM2 antibody    Western blot analysis of extracts from SKOV3 cells, using MDM2 antibody (#C0261).
anti-MDM2 antibody Testing Data #2 image
Section Bar
 Testing Data #3 of anti-MDM2 antibody    Peptide - +
Immunofluorescence analysis of A549 cells, using MDM2 antibody (#C0261).
anti-MDM2 antibody Testing Data #3 image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for MDM2. It may not necessarily be applicable to this product.
 NCBI GI #    223890205
 NCBI GeneID    4193
 NCBI Accession #    NP_001138811.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001145339.2 [Other Products]
Section Bar
 UniProt Primary Accession #    Q00987 [Other Products]
 UniProt Secondary Accession #    Q13226; Q13297; Q13298; Q13299; Q13300; Q13301; Q53XW0; Q71TW9; Q8WYJ1; A6NL51; A8K2S6 [Other Products]
 UniProt Related Accession #    Q00987 [Other Products]
 Molecular Weight    55,991 Da
Table BarTOPTable Bar
 NCBI Official Full Name    E3 ubiquitin-protein ligase Mdm2 isoform h
 NCBI Official Synonym Full Names    MDM2 proto-oncogene, E3 ubiquitin protein ligase
 NCBI Official Symbol    MDM2 [Similar Products]
 NCBI Official Synonym Symbols   
HDMX; hdm2; ACTFS
[Similar Products]
 NCBI Protein Information    E3 ubiquitin-protein ligase Mdm2; oncoprotein Mdm2; MDM2 oncogene, E3 ubiquitin protein ligase; Mdm2, p53 E3 ubiquitin protein ligase homolog; double minute 2, human homolog of; p53-binding protein; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein
 UniProt Protein Name    E3 ubiquitin-protein ligase Mdm2
 UniProt Synonym Protein Names   
Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2
 Protein Family    Mdm2-binding protein
 UniProt Gene Name    MDM2 [Similar Products]
 UniProt Synonym Gene Names    Hdm2 [Similar Products]
 UniProt Entry Name    MDM2_HUMAN
Section Bar
 NCBI Summary for MDM2    This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]
Section Bar
 UniProt Comments for MDM2    MDM2: a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 via Akt-mediated MDM2 phosphorylation. Phosphorylation of MDM2 increases its interaction with p300, providing a platform to allow the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. Facilitates the nuclear export of p53 and targets it for proteasome-mediated proteolysis. Eight alternatively spliced isoforms have been reported.

Protein type: EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-; Nucleolus; Inhibitor; Oncoprotein

Chromosomal Location of Human Ortholog: 12q14.3-q15

Cellular Component: nucleoplasm; nuclear body; protein complex; cytoplasm; nucleolus; plasma membrane; synapse; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; peroxisome proliferator activated receptor binding; enzyme binding; zinc ion binding; p53 binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; response to morphine; protein ubiquitination; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; response to carbohydrate stimulus; response to antibiotic; positive regulation of protein export from nucleus; synaptic transmission; negative regulation of DNA damage response, signal transduction by p53 class mediator; response to magnesium ion; establishment of protein localization; peptidyl-lysine modification; positive regulation of cell proliferation; protein complex assembly; response to iron ion; response to drug; epidermal growth factor receptor signaling pathway; traversing start control point of mitotic cell cycle; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; protein destabilization; response to ether; response to cocaine; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; innate immune response; regulation of protein catabolic process; negative regulation of transcription, DNA-dependent

Disease: Accelerated Tumor Formation, Susceptibility To
Table BarTOPTable Bar
 

Product References and Citations for anti-MDM2 antibody

   Jer-Yen Yang, Mol. Cell. Biol., Oct 2006; 26: 7269 - 7282.
Lubing Gu, Blood, May 2002; 99: 3367 - 3375.
Hongyan Zhu, J. Biol. Chem., Dec 2003; 278: 49286 - 49292.
Tatiana Dilla, Endocrinology, Jan 2000; 141: 420.
Section Bar
 Research Articles on MDM2    1. MDM2 is a direct target of miR-221, and a feed-forward loop is described that sustains miR-221 aberrant expression. Also, miR-221 can activate the p53/mdm2 axis by inhibiting MDM2 and, in turn, p53 activation contributes to miR-221 enhanced expression.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with anti-MDM2 antibodyDiseases associated with anti-MDM2 antibody
 Products by Pathway  Pathway Diagram
 AKT Phosphorylates Targets In The Cytosol Pathway antibodies  AKT Phosphorylates Targets In The Cytosol Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Aurora A Signaling Pathway antibodies  Aurora A Signaling Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >887 publications with MDM2 and Carcinoma
 Adenocarcinoma Antibodies  >369 publications with MDM2 and Adenocarcinoma
 Breast Neoplasms Antibodies  >318 publications with MDM2 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >280 publications with MDM2 and Cell Transformation, Neoplastic
 Nervous System Diseases Antibodies  >246 publications with MDM2 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >242 publications with MDM2 and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >231 publications with MDM2 and Lung Neoplasms
 Carcinoma, Squamous Cell Antibodies  >188 publications with MDM2 and Carcinoma, Squamous Cell
 Brain Diseases Antibodies  >182 publications with MDM2 and Brain Diseases
 Liver Neoplasms Antibodies  >146 publications with MDM2 and Liver Neoplasms
Organs/Tissues associated with anti-MDM2 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >375 publications with MDM2 and Blood
 Lung Antibodies  >317 publications with MDM2 and Lung
 Brain Antibodies  >243 publications with MDM2 and Brain
 Bone Antibodies  >239 publications with MDM2 and Bone
 Liver Antibodies  >219 publications with MDM2 and Liver
 Prostate Antibodies  >151 publications with MDM2 and Prostate
 Muscle Antibodies  >151 publications with MDM2 and Muscle
 Vascular Antibodies  >145 publications with MDM2 and Vascular
 Skin Antibodies  >124 publications with MDM2 and Skin
 Kidney Antibodies  >106 publications with MDM2 and Kidney
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions