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| Product Name
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MER/SKY, Antibody ★Popular Item★
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| Also Known As
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MER/SKY (Phospho-Tyr749/681) Antibody
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| Product Synonym Names
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C-mer; EC 2.7.10.1; kinase Mer; MERK; MERTK; proto-oncogene tyrosine-protein kinase MER precursor; receptor tyrosine kinase MerTK
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| OMIM
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604705
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| 3D Structure
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ModBase 3D Structure for Q12866
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| Species Reactivity
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Human, Mouse, Rat
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| Specificity
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MER/SKY (Phospho-Tyr749/681) antibody detects endogenous levels of MER/SKY only when phosphorylated at tyrosine 749/681.
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| Purity/Purification
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Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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| Form/Format
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In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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| Immunogen
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The antiserum was produced against synthesized phosphopeptide derived from human MER/SKY around the phosphorylation site of tyrosine 749/681 (K-I-YP-S-G).
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| All Sites
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Human: Tyr749/681
Mouse: Tyr744/671
Rat: Tyr744/671
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| Preparation and Storage
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Store at-20 degree C for 1 year.
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| Other Notes
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Small volumes of anti-MER/SKY antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Product Categories/Family for anti-MER/SKY antibody
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Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
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| Applications Tested/Suitable for anti-MER/SKY antibody
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Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
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| Application Notes for anti-MER/SKY antibody
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WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000
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| Testing Data of anti-MER/SKY antibody
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P-peptide-+
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| Immunohistochemistry (IHC) of anti-MER/SKY antibody
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using MER/SKY (Phospho-Tyr749/681) antibody. Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 5mins), using MER/SKY (Phospho-Tyr749/681) antibody.
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| NCBI/Uniprot data below describe general gene information for MER/SKY. It may not necessarily be applicable to this product. |
| NCBI GI #
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66932918
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| NCBI GeneID
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10461
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| NCBI Accession #
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NP_006334.2
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_006343.2
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[Other Products]
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| UniProt Primary Accession #
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Q12866
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[Other Products]
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| UniProt Secondary Accession #
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Q9HBB4
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[Other Products]
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| UniProt Related Accession #
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Q12866
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[Other Products]
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| Molecular Weight
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110,249 Da
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TOP
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| NCBI Official Full Name
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tyrosine-protein kinase Mer
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| NCBI Official Synonym Full Names
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MER proto-oncogene, tyrosine kinase
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| NCBI Official Symbol
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MERTK
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[Similar Products]
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| NCBI Official Synonym Symbols
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MER; RP38; c-Eyk; c-mer; Tyro12
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[Similar Products]
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| NCBI Protein Information
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tyrosine-protein kinase Mer
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| UniProt Protein Name
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Tyrosine-protein kinase Mer
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| UniProt Synonym Protein Names
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Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK
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| UniProt Gene Name
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MERTK
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[Similar Products]
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| UniProt Synonym Gene Names
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MER
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[Similar Products]
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| NCBI Summary for MER/SKY
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This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP). [provided by RefSeq, Jul 2008]
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| UniProt Comments for MER/SKY
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Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
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Graham D.K., Cell Growth Differ. 5:647-657(1994). Gal A., Nat. Genet. 26:270-271(2000). Hillier L.W., Nature 434:724-731(2005).
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| Research Articles on MER/SKY
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1. Study describes a novel cellular pathway involved in diabetic efferocytosis, wherein diabetes-induced decrease in miR-126 expression results in upregulation of ADAM9 expression that in-turn leads proteolytic cleavage of MerTK and formation of inactive soluble Mer. Decrease in MerTK phosphorylation leads to reduced downstream cytoskeletal signaling required for engulfment and thus decreases efferocytosis.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with anti-MER/SKY antibody | Pathways associated with anti-MER/SKY antibody |
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| Diseases associated with anti-MER/SKY antibody | Organs/Tissues associated with anti-MER/SKY antibody |
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Antibody 
