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MUC1 antibody :: MUC1 / EMA /CD227 Antibody

Scan QR to view Datasheet Catalog #    MBS439543
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
7 ml (Prediluted)  /  $270 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MUC1 / EMA /CD227, Antibody

★Popular Item★
 Also Known As   

MUC1 / EMA /CD227 (Epithelial Marker) Mouse Monoclonal Antibody

 Product Synonym Names    Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen, Tumor-associated mucin
 Product Gene Name   

MUC1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    113720
 3D Structure    ModBase 3D Structure for P15941
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 Clonality    Monoclonal
 Isotype    IgG2a, kappa
 Clone Number    MUC1/520
 Host    Mouse
 Species Reactivity    Human
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 Specificity    In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
 Form/Format    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
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 Cellular Localization    Cytoplasmic & Cell Surface
 Immunogen    Human milk-fat globule membranes (HMFGM)
 Hu-Chromosome Location    1q22
 Positive Control    MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.
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 Preparation and Storage    Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
 Other Notes    Small volumes of MUC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for MUC1 antibody   

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

 Application Notes for MUC1 antibody    Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
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 Immunohistochemistry (IHC) of MUC1 antibody    Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).
MUC1 antibody  Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of MUC1 antibody    Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).
MUC1 antibody  Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of MUC1 antibody    Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).
MUC1 antibody  Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of MUC1 antibody    Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).
MUC1 antibody  Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of MUC1 antibody    Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).
MUC1 antibody  Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of MUC1 antibody    Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).
MUC1 antibody  Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for MUC1. It may not necessarily be applicable to this product.
 NCBI GI #    67189007
 NCBI GeneID    4582
 NCBI Accession #    NP_001018016.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001018016.2 [Other Products]
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 UniProt Primary Accession #    P15941 [Other Products]
 UniProt Secondary Accession #    P13931; P15942; P17626; A5YRV1; A6ZID9; A6ZIE0; B1AVQ8; B1AVR0; B6ECA1; E7ESE5; E7EUG9 [Other Products]
 UniProt Related Accession #    P15941 [Other Products]
 Molecular Weight    265-400kDa
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 NCBI Official Full Name    mucin-1 isoform 2
 NCBI Official Synonym Full Names    mucin 1, cell surface associated
 NCBI Official Symbol    MUC1 [Similar Products]
 NCBI Official Synonym Symbols   
EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
[Similar Products]
 NCBI Protein Information    mucin-1
 UniProt Protein Name    Mucin-1
 UniProt Synonym Protein Names   
Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3
 Protein Family    Mucin
 UniProt Gene Name    MUC1 [Similar Products]
 UniProt Synonym Gene Names    PUM; MUC-1; CA 15-3; KL-6; PUM; PEM; EMA; MUC1-NT; MUC1-alpha; MUC1-beta [Similar Products]
 UniProt Entry Name    MUC1_HUMAN
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 NCBI Summary for MUC1    This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]
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 UniProt Comments for MUC1    MUC1: a large cell surface glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. Plays a role in adhesion and cell-cell interactions, metastasis and signaling. May provide a protective layer on epithelial surfaces. Direct or indirect interaction with actin cytoskeleton. Its cytoplasmic tail (MUC1CT) is involved in several signaling pathways, including those involving Ras, beta-catenin, p120 catenin, p53 and estrogen receptor alpha. MUC1CT also forms complexes with transcription factors, and then translocates to the nucleus by an unknown mechanism, where it is believed to influence the transcription of their target genes. MUC1CT has also been proposed to localize to mitochondrial membranes under conditions of genotoxic stress, where it attenuates the apoptotic pathway in response and confers resistance to apoptosis-inducing drugs. Aberrantly glycosylated forms expressed in human epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Nine alternatively spliced isoforms have been described. Isoforms 5 and 9 are secreted. Isoform 7, expressed only in tumor cells, is a receptor and binds the secreted isoform 5. The binding induces the phosphorylation of the isoform 7, alters cellular morphology and initiates cell signaling. Can bind to GRB2 adapter protein.

Protein type: Nuclear receptor co-regulator; Actin-binding; Tumor suppressor; Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; Golgi lumen; integral to plasma membrane; nuclear chromatin; vesicle

Molecular Function: p53 binding; protein binding; transcription cofactor activity

Biological Process: DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; negative regulation of cell adhesion mediated by integrin; O-glycan processing; regulation of transcription from RNA polymerase II promoter in response to stress

Disease: Medullary Cystic Kidney Disease 1
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Product References and Citations for MUC1 antibody

   Stanley CM, Phillips TE. Am J Physiol. 1999;277:G191-200
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 Research Articles on MUC1    1. Serum KL-6 was not significantly different between patients with connective tissue disease-interstitial lung disease, chronic fibrosing interstitial pneumonia patients and healthy controls.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MUC1 antibody Pathways associated with MUC1 antibody
 Reference Product  PubMed Publications
 MUC2 antibody  >613 publications with MUC1 and MUC2
 MUC5AC antibody  >476 publications with MUC1 and MUC5AC
 MUC4 antibody  >312 publications with MUC1 and MUC4
 MUC6 antibody  >291 publications with MUC1 and MUC6
 CTNNB1 antibody  >129 publications with MUC1 and CTNNB1
 EGFR antibody  >109 publications with MUC1 and EGFR
 MUC16 antibody  >101 publications with MUC1 and MUC16
 SRC antibody  >39 publications with MUC1 and SRC
 MUC15 antibody  >10 publications with MUC1 and MUC15
 Products by Pathway  Pathway Diagram
 Defective C1GALT1C1 Causes Tn Polyagglutination Syndrome (TNPS) Pathway antibodies  Defective C1GALT1C1 Causes Tn Polyagglutination Syndrome (TNPS) Pathway Diagram
 Defective GALNT12 Causes Colorectal Cancer 1 (CRCS1) Pathway antibodies  Defective GALNT12 Causes Colorectal Cancer 1 (CRCS1) Pathway Diagram
 Defective GALNT3 Causes Familial Hyperphosphatemic Tumoral Calcinosis (HFTC) Pathway antibodies  Defective GALNT3 Causes Familial Hyperphosphatemic Tumoral Calcinosis (HFTC) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With O-glycosylation Of Proteins Pathway antibodies  Diseases Associated With O-glycosylation Of Proteins Pathway Diagram
 Diseases Of Glycosylation Pathway antibodies  Diseases Of Glycosylation Pathway Diagram
 IL-7 Signaling Pathway antibodies  IL-7 Signaling Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 O-linked Glycosylation Pathway antibodies  O-linked Glycosylation Pathway Diagram
 O-linked Glycosylation Of Mucins Pathway antibodies  O-linked Glycosylation Of Mucins Pathway Diagram
Diseases associated with MUC1 antibody Organs/Tissues associated with MUC1 antibody
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >659 publications with MUC1 and Breast Neoplasms
 Lung Diseases Antibodies  >492 publications with MUC1 and Lung Diseases
 Lung Neoplasms Antibodies  >222 publications with MUC1 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >188 publications with MUC1 and Neoplasms, Experimental
 Ovarian Neoplasms Antibodies  >182 publications with MUC1 and Ovarian Neoplasms
 Disease Models, Animal Antibodies  >181 publications with MUC1 and Disease Models, Animal
 Stomach Neoplasms Antibodies  >162 publications with MUC1 and Stomach Neoplasms
 Fibrosis Antibodies  >153 publications with MUC1 and Fibrosis
 Inflammation Antibodies  >138 publications with MUC1 and Inflammation
 Pneumonia Antibodies  >138 publications with MUC1 and Pneumonia
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >580 publications with MUC1 and Lung
 Stomach Antibodies  >230 publications with MUC1 and Stomach
 Pancreas Antibodies  >223 publications with MUC1 and Pancreas
 Liver Antibodies  >200 publications with MUC1 and Liver
 Intestine Antibodies  >168 publications with MUC1 and Intestine
 Prostate Antibodies  >111 publications with MUC1 and Prostate
 Eye Antibodies  >108 publications with MUC1 and Eye
 Kidney Antibodies  >107 publications with MUC1 and Kidney
 Uterus Antibodies  >84 publications with MUC1 and Uterus
 Ovary Antibodies  >63 publications with MUC1 and Ovary
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