View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List Mouse anti-Human 14-3-3, pan Monoclonal Antibody Catalog # MBS601777 Unit / Price DISCONTINUEDClick here to view our similar product(s) OR click here to contact our technical support team for suitable replacement. Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name 14-3-3, pan, Monoclonal Antibody Full Product Name 14-3-3, pan Product Synonym Names Anti -14-3-3, pan Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgG1,k Clone Number 3F7 Host Mouse Species Reactivity Human Specificity Recognizes human beta, gamma, sigma and zeta isoforms of 14.3.3. Purity/Purification Affinity PurifiedPurified by Protein G affinity chromatography. Form/Format Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See 0014-01X. Immunogen 14.3.3 gamma recombinant protein of human origin (26-33kD). Cellular Localization: Cytoplasmic. Positive Control HeLa cells Preparation and Storage May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Other Notes Small volumes of anti-14-3-3, pan antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-14-3-3, pan antibody 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. Product Categories/Family for anti-14-3-3, pan antibody Antibodies; Abs to 14-3-3 Applications Tested/Suitable for anti-14-3-3, pan antibody Western Blot (WB), Immunoprecipitation (IP) Application Notes for anti-14-3-3, pan antibody Suitable for use in Immunoprecipitation and Western Blot.Dilution: Immunoprecipitation (Denatured): 2ug/mg protein lysate using Protein G.Western Blot: 1-2ug/ml for 2 hours at RT Western Blot (WB) of anti-14-3-3, pan antibody Western Blot using MBS601777. Western Blot (WB) of anti-14-3-3, pan antibody Immunoprecipitation/ Western Blot using MBS601777. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP