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anti-14-3-3 antibody :: Rabbit 14-3-3 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS179850
Western Blot (WB)
Unit / Price
0.1 mg  /  $280 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

14-3-3, Monoclonal Antibody

 Also Known As   

Anti-14-3-3 Rabbit Monoclonal Antibody

 Product Synonym Names    14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1; 14-3-3 protein beta/alpha, N-terminally processed; YWHAB
 Product Gene Name   

anti-14-3-3 antibody

[Similar Products]
 Product Synonym Gene Name    YWHAB [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    X57346 mRNA
 3D Structure    ModBase 3D Structure for P31946
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 Clonality    Monoclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Purity/Purification    Affinity-chromatography
 Form/Format    Liquid
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 Immunogen    A synthesized peptide derived from human 41701
 Subcellular Localization    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
 Contents    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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 Preparation and Storage    Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-14-3-3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-14-3-3 antibody

   Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activatation of MAP kinases via AKAP13.
 Product Categories/Family for anti-14-3-3 antibody    Neuroscience; Neurology Process; Neural Signal Transduction; Signal Transduction; Adapters; Cytoplasmic; Neuroscience; Cell Type Marker; neuron marker; synapse marker; Stem Cells; Signaling Pathways; wnt; 14-3-3
 Applications Tested/Suitable for anti-14-3-3 antibody   

Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)

 Application Notes for anti-14-3-3 antibody    WB: 1:500-1:1000
IHC: 1:50-1:200
FC/FACS: 1:50
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 Western Blot (WB) of anti-14-3-3 antibody    Western blot analysis of 14-3-3 expression in Hela lysate (MBS179850).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for YWHAB
anti-14-3-3 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for 14-3-3. It may not necessarily be applicable to this product.
 NCBI GI #    4507949
 NCBI GeneID    7529
 NCBI Accession #    NP_003395.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_003404.4 [Other Products]
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 UniProt Primary Accession #    P31946 [Other Products]
 UniProt Secondary Accession #    A8K9K2; E1P616 [Other Products]
 UniProt Related Accession #    P31946 [Other Products]
 Molecular Weight    28082 MW
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 NCBI Official Full Name    14-3-3 protein beta/alpha
 NCBI Official Synonym Full Names    tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta
 NCBI Official Symbol    YWHAB [Similar Products]
 NCBI Official Synonym Symbols   
HS1; GW128; YWHAA; KCIP-1; HEL-S-1
[Similar Products]
 NCBI Protein Information    14-3-3 protein beta/alpha
 UniProt Protein Name    14-3-3 protein beta/alpha
 UniProt Synonym Protein Names   
Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1
 Protein Family    14-3-3 protein
 UniProt Gene Name    YWHAB [Similar Products]
 UniProt Synonym Gene Names    KCIP-1 [Similar Products]
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 NCBI Summary for 14-3-3    This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for 14-3-3    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
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 Research Articles on 14-3-3    1. Data show that 14-3-3beta protein augmented the expression of matrix metalloproteinasea MMP2 and MMP9 through PI3 kinase/Akt protein/NF-kappaappa B pathway, thereby enhancing the invasiveness of hepatocellular carcinoma (HCC) cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-14-3-3 antibodyPathways associated with anti-14-3-3 antibody
 Reference Product  PubMed Publications
 CDC25B antibody  >3 publications with 14-3-3 and CDC25B
 CDC25A antibody  >3 publications with 14-3-3 and CDC25A
 IGF1R antibody  >3 publications with 14-3-3 and IGF1R
 CDC25C antibody  >2 publications with 14-3-3 and CDC25C
 YWHAE antibody  >2 publications with 14-3-3 and YWHAE
 SRC antibody  >2 publications with 14-3-3 and SRC
 KCNK3 antibody  >1 publications with 14-3-3 and KCNK3
 EPB41L1 antibody  >1 publications with 14-3-3 and EPB41L1
 BAD antibody  >1 publications with 14-3-3 and BAD
 TSC2 antibody  >1 publications with 14-3-3 and TSC2
 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
Diseases associated with anti-14-3-3 antibodyOrgans/Tissues associated with anti-14-3-3 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >15 publications with 14-3-3 and Neoplasms
 Nervous System Diseases Antibodies  >11 publications with 14-3-3 and Nervous System Diseases
 Carcinoma Antibodies  >6 publications with 14-3-3 and Carcinoma
 Liver Diseases Antibodies  >3 publications with 14-3-3 and Liver Diseases
 Intestinal Neoplasms Antibodies  >3 publications with 14-3-3 and Intestinal Neoplasms
 Cardiovascular Diseases Antibodies  >2 publications with 14-3-3 and Cardiovascular Diseases
 Inflammation Antibodies  >2 publications with 14-3-3 and Inflammation
 Liver Neoplasms Antibodies  >2 publications with 14-3-3 and Liver Neoplasms
 Neoplasm Metastasis Antibodies  >2 publications with 14-3-3 and Neoplasm Metastasis
 Seizures Antibodies  >2 publications with 14-3-3 and Seizures
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >11 publications with 14-3-3 and Brain
 Blood Antibodies  >4 publications with 14-3-3 and Blood
 Muscle Antibodies  >4 publications with 14-3-3 and Muscle
 Kidney Antibodies  >3 publications with 14-3-3 and Kidney
 Liver Antibodies  >3 publications with 14-3-3 and Liver
 Nerve Antibodies  >3 publications with 14-3-3 and Nerve
 Lung Antibodies  >2 publications with 14-3-3 and Lung
 Ganglia Antibodies  >2 publications with 14-3-3 and Ganglia
 Vascular Antibodies  >2 publications with 14-3-3 and Vascular
 Heart Antibodies  >2 publications with 14-3-3 and Heart
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