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anti-A-RAF antibody :: Rabbit anti-Human A-RAF Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS179793
Western Blot (WB)
Unit / Price
0.1 mg  /  $280 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

A-RAF, Monoclonal Antibody

 Also Known As   

Anti-A-RAF Rabbit Monoclonal Antibody

 Product Synonym Names    Serine/threonine-protein kinase A-Raf; 2.7.11.1; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks; ARAF; ARAF1; PKS; PKS2
 Product Gene Name   

anti-A-RAF antibody

[Similar Products]
 Product Synonym Gene Name    ARAF [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    X04790 mRNA
 3D Structure    ModBase 3D Structure for P10398
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 Clonality    Monoclonal
 Host    Rabbit
 Species Reactivity    Human
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 Purity/Purification    Affinity-chromatography
 Form/Format    Liquid
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 Immunogen    A synthesized peptide derived from human A-RAF
 Tissue Specificity    Predominantly in urogenital tissues.
 Contents    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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 Preparation and Storage    Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-A-RAF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-A-RAF antibody

   Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.
 Applications Tested/Suitable for anti-A-RAF antibody   

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

 Application Notes for anti-A-RAF antibody    WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50
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 Western Blot (WB) of anti-A-RAF antibody    Western blot analysis of A-RAF expression in A375 cell lysate (MBS179793).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARAF monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ARAF
anti-A-RAF antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for A-RAF. It may not necessarily be applicable to this product.
 NCBI GI #    371875793
 NCBI GeneID    369
 NCBI Accession #    NP_001243125.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001256196.1 [Other Products]
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 UniProt Primary Accession #    P10398 [Other Products]
 UniProt Secondary Accession #    P07557; Q5H9B2; Q5H9B3 [Other Products]
 UniProt Related Accession #    P10398 [Other Products]
 Molecular Weight    67585 MW
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 NCBI Official Full Name    serine/threonine-protein kinase A-Raf isoform 2
 NCBI Official Synonym Full Names    A-Raf proto-oncogene, serine/threonine kinase
 NCBI Official Symbol    ARAF [Similar Products]
 NCBI Official Synonym Symbols   
PKS2; A-RAF; ARAF1; RAFA1
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase A-Raf
 UniProt Protein Name    Serine/threonine-protein kinase A-Raf
 UniProt Synonym Protein Names   
Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks
 UniProt Gene Name    ARAF [Similar Products]
 UniProt Synonym Gene Names    ARAF1; PKS; PKS2 [Similar Products]
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 NCBI Summary for A-RAF    This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]
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 UniProt Comments for A-RAF    Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.
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 Research Articles on A-RAF    1. The constitutive or induced re-localization of A-Raf to the plasma membrane compromises its ability to efficiently sequester and inactivate MST2, thus rendering cells susceptible to apoptosis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-A-RAF antibodyPathways associated with anti-A-RAF antibody
 Reference Product  PubMed Publications
 HRAS antibody  >3 publications with A-RAF and HRAS
 PIK3R1 antibody  >1 publications with A-RAF and PIK3R1
 Products by Pathway  Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Colorectal Cancer Pathway antibodies  Colorectal Cancer Pathway Diagram
 Colorectal Cancer Pathway antibodies  Colorectal Cancer Pathway Diagram
Diseases associated with anti-A-RAF antibodyOrgans/Tissues associated with anti-A-RAF antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >11 publications with A-RAF and Neoplasms
 Necrosis Antibodies  >5 publications with A-RAF and Necrosis
 Inflammation Antibodies  >3 publications with A-RAF and Inflammation
 Fibrosis Antibodies  >2 publications with A-RAF and Fibrosis
 Drug Toxicity Antibodies  >2 publications with A-RAF and Drug Toxicity
 Adenocarcinoma Antibodies  >2 publications with A-RAF and Adenocarcinoma
 Weight Loss Antibodies  >2 publications with A-RAF and Weight Loss
 Lung Diseases Antibodies  >1 publications with A-RAF and Lung Diseases
 Heart Diseases Antibodies  >1 publications with A-RAF and Heart Diseases
 Hypertension Antibodies  >1 publications with A-RAF and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >14 publications with A-RAF and Blood
 Vascular Antibodies  >5 publications with A-RAF and Vascular
 Spleen Antibodies  >4 publications with A-RAF and Spleen
 Thyroid Antibodies  >3 publications with A-RAF and Thyroid
 Bone Antibodies  >3 publications with A-RAF and Bone
 Eye Antibodies  >3 publications with A-RAF and Eye
 Kidney Antibodies  >2 publications with A-RAF and Kidney
 Lung Antibodies  >2 publications with A-RAF and Lung
 Mouth Antibodies  >2 publications with A-RAF and Mouth
 Skin Antibodies  >2 publications with A-RAF and Skin
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