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anti-ADAM15 antibody :: Mouse anti-Human ADAM15 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS607344 anti-ADAM15 antibody
Unit / Price
0.1 mg  /  $630 +1 FREE 8GB USB
0.5 mg  /  $1,215 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ADAM15, Monoclonal Antibody

 Also Known As   

ADAM15 (a Disintegrin and Metalloprotease 15, Metargidin, MDC15)

 Product Synonym Names    Anti -ADAM15 (a Disintegrin and Metalloprotease 15, Metargidin, MDC15)
 Product Gene Name   

anti-ADAM15 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    chromosome: 1; Location: 1q21.3
 OMIM    605548
 3D Structure    ModBase 3D Structure for Q13444
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    9H33
 Host    Mouse
 Species Reactivity    Human
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 Specificity    Recognizes human ADAM15 ectodomain.
 Purity/Purification    Affinity Purified
Purified by Protein G affinity chromatography.
 Form/Format    Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
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 Immunogen    Recombinant protein corresponding to aa207-696 from human ADAM15 ectodomain fused to the IgG Fc domain expressed in COS-7 cells. (Accession #Q13444)
 Positive Control    MCF-7 cells
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 Preparation and Storage    Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of anti-ADAM15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-ADAM15 antibody

   ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.
 Product Categories/Family for anti-ADAM15 antibody    Antibodies; Abs to Matrix Metalloproteinases
 Applications Tested/Suitable for anti-ADAM15 antibody   

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

 Application Notes for anti-ADAM15 antibody    Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunocytochemistry and Direct ELISA.
Dilution: Flow Cytometry: 25ug/ml. 10ul labels 1-2.5x10e5 cells in a total reaction 200ul.
Immunocytochemistry: 10ug/ml. Cells fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100 and 1% BSA. After blocking, cells were incubated with diluted A0859-31B followed by labeled anti-mouse IgG.
Western Blot: 1-2ug/ml. Detects ~5ng/lane of rhADAM15 under non-reducing conditions.
Immunoprecipitation: 25ug/ml
Direct ELISA: 1ug/ml detects ~0.3ng rhADAM15/well.
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NCBI/Uniprot data below describe general gene information for ADAM15. It may not necessarily be applicable to this product.
 NCBI GI #    55960136
 NCBI GeneID    8751
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 UniProt Primary Accession #    Q13444 [Other Products]
 UniProt Secondary Accession #    Q13493; Q53XQ0; Q5SR68; Q5SR69; Q6R267; Q71S61; Q71S62; Q71S63; Q71S64; Q71S65; B3KQU5 [Other Products]
 UniProt Related Accession #    Q13444; Q59GF2 [Other Products]
 Molecular Weight    92,959 Da [Similar Products]
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 NCBI Official Full Name    ADAM metallopeptidase domain 15
 NCBI Official Synonym Full Names    ADAM metallopeptidase domain 15
 NCBI Official Symbol    ADAM15 [Similar Products]
 NCBI Official Synonym Symbols   
MDC15; ADAM15
[Similar Products]
 NCBI Protein Information    disintegrin and metalloproteinase domain-containing protein 15; MDC-15; ADAM 15; metargidin; OTTHUMP00000033236; metalloprotease RGD disintegrin protein; a disintegrin and metalloproteinase domain 15 (metargidin); metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15
 UniProt Protein Name    Disintegrin and metalloproteinase domain-containing protein 15
 UniProt Synonym Protein Names   
Metalloprotease RGD disintegrin protein; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15; MDC-15; Metargidin
 Protein Family    Disintegrin and metalloproteinase domain-containing protein
 UniProt Gene Name    ADAM15 [Similar Products]
 UniProt Synonym Gene Names    MDC15 [Similar Products]
 UniProt Entry Name    ADA15_HUMAN
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 NCBI Summary for ADAM15    The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]
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 UniProt Comments for ADAM15    ADAM15: Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis; EC 3.4.24.-; Protease

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cell projection; adherens junction; cell surface; acrosome; integral to membrane; plasma membrane

Molecular Function: integrin binding; protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; SH3 domain binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; apoptosis; cell-matrix adhesion; proteolysis; negative regulation of cell-matrix adhesion; collagen catabolic process; extracellular matrix disassembly; tissue regeneration; innate immune response; negative regulation of cell growth; angiogenesis; negative regulation of cell migration
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 Research Articles on ADAM15    1. ADAM15 conveys antiapoptotic properties to osteoarthritis chondrocytes that might sustain their potential to better resist the influence of death-inducing stimuli under pathophysiologic conditions
2. Alternative splicing of ADAM15 regulates its interactions with cellular SH3 proteins SNX33 and nephrocystin
3. analysis of the ADAM15 disintegrin domain and its binding properties
4. Insights into the mechanism of how a splice variant linked to clinical agressiveness in breast cancer causes increased activity of ADAM15B.
5. involved in advanced atherosclerosis, in catalytically active form, most notably associated with cells of monocytic origin
6. results define key catalytic properties of ADAM15 in cells and its response to stimulators and inhibitors of ectodomain shedding.
7. Observational study of gene-disease association. (HuGE Navigator)
8. Recombinant ADAM15 disintegrin domain inhibits melanoma cell proliferation partly through p38 kinase activation.
9. This presents a novel mechanism by which ADAM15 regulates cell-matrix adhesion and migration.
10. ADAM15 catalyzes the cleavage of E-cadherin to generate a soluble fragment that in turn binds to and stimulates ErbB receptor signaling
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ADAM15 antibodyDiseases associated with anti-ADAM15 antibody
 Reference Product  PubMed Publications
 SRC antibody  >15 publications with ADAM15 and SRC
 LCK antibody  >4 publications with ADAM15 and LCK
 HCK antibody  >2 publications with ADAM15 and HCK
 GRB2 antibody  >2 publications with ADAM15 and GRB2
 SH3D19 antibody  >1 publications with ADAM15 and SH3D19
 SNX9 antibody  >1 publications with ADAM15 and SNX9
 SH3GL2 antibody  >1 publications with ADAM15 and SH3GL2
 NPHP1 antibody  >1 publications with ADAM15 and NPHP1
 FYN antibody  >1 publications with ADAM15 and FYN
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >29 publications with ADAM15 and Neoplasms
 Cardiovascular Diseases Antibodies  >9 publications with ADAM15 and Cardiovascular Diseases
 Melanoma Antibodies  >7 publications with ADAM15 and Melanoma
 Neovascularization, Pathologic Antibodies  >7 publications with ADAM15 and Neovascularization, Pathologic
 Breast Neoplasms Antibodies  >6 publications with ADAM15 and Breast Neoplasms
 Adenocarcinoma Antibodies  >5 publications with ADAM15 and Adenocarcinoma
 Lung Neoplasms Antibodies  >5 publications with ADAM15 and Lung Neoplasms
 Prostatic Neoplasms Antibodies  >4 publications with ADAM15 and Prostatic Neoplasms
 Autoimmune Diseases Antibodies  >3 publications with ADAM15 and Autoimmune Diseases
 Lung Diseases Antibodies  >3 publications with ADAM15 and Lung Diseases
Organs/Tissues associated with anti-ADAM15 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >17 publications with ADAM15 and Blood
 Vascular Antibodies  >16 publications with ADAM15 and Vascular
 Muscle Antibodies  >12 publications with ADAM15 and Muscle
 Lung Antibodies  >8 publications with ADAM15 and Lung
 Heart Antibodies  >8 publications with ADAM15 and Heart
 Prostate Antibodies  >6 publications with ADAM15 and Prostate
 Bone Antibodies  >6 publications with ADAM15 and Bone
 Brain Antibodies  >6 publications with ADAM15 and Brain
 Testis Antibodies  >4 publications with ADAM15 and Testis
 Intestine Antibodies  >3 publications with ADAM15 and Intestine
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