Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow ALIX rightarrow Monoclonal ALIX  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • chromosome 20 open reading frame 114 (C20orf114) ELISA Kit
 • XBP1 Antibody
 • ALBUMIN Antibody
 • patched homolog 1 (Drosophila) (PTCH1) ELISA Kit
 • Lactate Dehydrogenase (LDH) Protein
 • Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ELISA Kit
 • Thrombopoietin (THPO) Antibody
 • TNFRSF1A ELISA Kit
 • Dehydroepiandrosterone (DHEA) Antibody
 • Human IgG1 lambda Isotype Control Isotype Control
 • ADAMTS-like 2 (ADAMTSL2) ELISA Kit
 • CD40 Antibody
 • Catenin-beta (CTNNB1) Antibody
 • achaete-scute complex homolog 1 (Drosophila) (ASCL1) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-ALIX antibody :: Mouse ALIX Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS212843
Testing Data
Unit / Price
0.1 mg  /  $390 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ALIX, Monoclonal Antibody

 Also Known As   

MOUSE ANTI ALIX

 Product Gene Name   

anti-ALIX antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    608074
 3D Structure    ModBase 3D Structure for Q8WUM4
Section Bar
 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    3A9
 Host    Mouse
 Species Reactivity    Human, Mouse, Rat, Xenopus
Section Bar
 Form/Format    Purified
Purified IgG - liquid
 Concentration    IgG concentration 1.0mg/ml (lot specific)
Section Bar
 Perservative Stabilisers    0.09% Sodium Azide (NaN3)
Preparation
 Buffer Solution    Phosphate buffered saline
Section Bar
 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-ALIX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Product Description specifically for anti-ALIX antibody

   Mouse anti ALIX antibody, clone 3A9 recognizes the apoptosis-linked gene-2 interacting protein X (ALIX), also known as Programmed cell death 6-interacting protein or Hp95. ALIX is a 868 amino acid ~90kDa cytoplasmic protein containing a single BRO1 domain implicated in endosomal targeting (Kim et al. 2005). ALIX was first reported to play a role in apoptosis dependent on its association with PDCD6 (Mahul-Mellier et al. 2006) but has subsequently shown to be involved in endocytosis (Matsuo et al. 2004), viral budding (Irie et al. 2008) and actin cytoskeletal assembly (Pan et al. 2006). Mouse anti Alix, clone 3A9 has been used extensively for the detection of ALIX by Western blotting and for the identification of an ALIX homologue in the sea urchin Paracentrotus lividus (Romancino et al. 2013).
 Applications Tested/Suitable for anti-ALIX antibody   

ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin, Western Blot (WB)

 Application Notes for anti-ALIX antibody    Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections.
Section Bar
 Testing Data of anti-ALIX antibody    Western blot analysis of HeLa whole cell lysate probed with MOUSE ANTI ALIX F(ab')2 Rabbit anti Mouse IgG:HRP (MBS235192)
anti-ALIX antibody Testing Data image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for ALIX. It may not necessarily be applicable to this product.
 NCBI GI #    241982780
 NCBI GeneID    10015
 NCBI Accession #    NP_001155901.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001162429.2 [Other Products]
Section Bar
 UniProt Primary Accession #    Q8WUM4 [Other Products]
 UniProt Secondary Accession #    Q6NUS1; Q9BX86; Q9NUN0; Q9P2H2; Q9UKL5; C5MQH7; E9PFU1 [Other Products]
 UniProt Related Accession #    Q8WUM4 [Other Products]
 Molecular Weight    30,453 Da
Table BarTOPTable Bar
 NCBI Official Full Name    programmed cell death 6-interacting protein isoform 2
 NCBI Official Synonym Full Names    programmed cell death 6 interacting protein
 NCBI Official Symbol    PDCD6IP [Similar Products]
 NCBI Official Synonym Symbols   
AIP1; ALIX; HP95; DRIP4
[Similar Products]
 NCBI Protein Information    programmed cell death 6-interacting protein; ALG-2 interacting protein 1; ALG-2-interacting protein X; PDCD6-interacting protein; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4
 UniProt Protein Name    Programmed cell death 6-interacting protein
 UniProt Synonym Protein Names   
ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95
 UniProt Gene Name    PDCD6IP [Similar Products]
 UniProt Synonym Gene Names    AIP1; ALIX; KIAA1375; PDCD6-interacting protein [Similar Products]
 UniProt Entry Name    PDC6I_HUMAN
Section Bar
 NCBI Summary for ALIX    This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]
Section Bar
 UniProt Comments for ALIX    Alix: an adaptor protein that may regulate the function of receptor and cytoskeleton-associated tyrosine kinases. A class E vacuolar protein sorting (VPS) factor involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. In case of infection, the HIV-1 virus directly takes advantage of its function for viral exocytosis and budding, via its interaction with HIV-1 p6 protein. May play a role in the regulation of both apoptosis and cell proliferation.

Protein type: Apoptosis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3p22.3

Cellular Component: focal adhesion; membrane; cytoplasm; melanosome; microtubule organizing center; immunological synapse; cytosol; vesicle

Molecular Function: protein binding; protein homodimerization activity; proteinase activated receptor binding; SH3 domain binding; calcium-dependent protein binding

Biological Process: protein transport; cell separation during cytokinesis; viral reproduction; apoptosis; viral infectious cycle; mitotic metaphase plate congression; nuclear organization and biogenesis
Table BarTOPTable Bar
 Research Articles on ALIX    1. ALIX is recruited to the neck of the assembling HIV-1 virion and is mostly recycled after virion release.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-ALIX antibodyPathways associated with anti-ALIX antibody
 Reference Product  PubMed Publications
 TSG101 antibody  >39 publications with ALIX and TSG101
 PDCD6 antibody  >18 publications with ALIX and PDCD6
 CHMP4B antibody  >14 publications with ALIX and CHMP4B
 CEP55 antibody  >10 publications with ALIX and CEP55
 CHMP4A antibody  >7 publications with ALIX and CHMP4A
 SH3GL2 antibody  >4 publications with ALIX and SH3GL2
 SH3KBP1 antibody  >3 publications with ALIX and SH3KBP1
 CHMP4C antibody  >2 publications with ALIX and CHMP4C
 ALG2 antibody  >1 publications with ALIX and ALG2
 Products by Pathway  Pathway Diagram
 Budding And Maturation Of HIV Virion Pathway antibodies  Budding And Maturation Of HIV Virion Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
 Late Phase Of HIV Life Cycle Pathway antibodies  Late Phase Of HIV Life Cycle Pathway Diagram
 Uptake And Actions Of Bacterial Toxins Pathway antibodies  Uptake And Actions Of Bacterial Toxins Pathway Diagram
 Uptake And Function Of Anthrax Toxins Pathway antibodies  Uptake And Function Of Anthrax Toxins Pathway Diagram
Diseases associated with anti-ALIX antibodyOrgans/Tissues associated with anti-ALIX antibody
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >4 publications with ALIX and Adenocarcinoma
 Kidney Diseases Antibodies  >4 publications with ALIX and Kidney Diseases
 Disease Models, Animal Antibodies  >3 publications with ALIX and Disease Models, Animal
 Carcinoma, Hepatocellular Antibodies  >2 publications with ALIX and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >2 publications with ALIX and Liver Neoplasms
 Liver Diseases Antibodies  >2 publications with ALIX and Liver Diseases
 Anoxia Antibodies  >1 publications with ALIX and Anoxia
 Prostatic Diseases Antibodies  >1 publications with ALIX and Prostatic Diseases
 Breast Neoplasms Antibodies  >1 publications with ALIX and Breast Neoplasms
 Edema Antibodies  >1 publications with ALIX and Edema
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >12 publications with ALIX and Brain
 Kidney Antibodies  >10 publications with ALIX and Kidney
 Blood Antibodies  >7 publications with ALIX and Blood
 Nerve Antibodies  >5 publications with ALIX and Nerve
 Muscle Antibodies  >4 publications with ALIX and Muscle
 Lung Antibodies  >4 publications with ALIX and Lung
 Heart Antibodies  >2 publications with ALIX and Heart
 Liver Antibodies  >2 publications with ALIX and Liver
 Intestine Antibodies  >2 publications with ALIX and Intestine
 Pancreas Antibodies  >1 publications with ALIX and Pancreas
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions