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anti-ALPP antibody :: Mouse anti-Human ALPP Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9200484
Unit / Price
0.1 mL  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ALPP, Monoclonal Antibody

 Also Known As   

ALPP Antibody (N-term) (Ascites)

 Product Synonym Names    Alkaline phosphatase; placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1; ALPP; PLAP
 Product Gene Name   

anti-ALPP antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence Positions    56-83
 OMIM    171800
 3D Structure    ModBase 3D Structure for P05187
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    421CT8.1.1
 Host    Mouse
 Species Reactivity    Human
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 Specificity    This ALPP antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 56-83 amino acids from the N-terminal region of human ALPP.
 Form/Format    Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
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 Antigen Type    Synthetic Peptide
 Antigen Source    HUMAN
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 Preparation and Storage    Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-ALPP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-ALPP antibody

   There are at least four distinct but related alkaline
phosphatases: intestinal, placental, placental-like, and
liver/bone/kidney (tissue non-specific). The first three are
located together on chromosome 2 while the tissue non-specific form
is located on chromosome 1. The product of this gene is a membrane
bound glycosylated enzyme, also referred to as the heat stable
form, that is expressed primarily in the placenta although it is
closely related to the intestinal form of the enzyme as well as to
the placental-like form. The coding sequence for this form of
alkaline phosphatase is unique in that the 3' untranslated region
contains multiple copies of an Alu family repeat. In addition, this
gene is polymorphic and three common alleles (type 1, type 2 and
type 3) for this form of alkaline phosphatase have been well
characterized.
 Product Categories/Family for anti-ALPP antibody    Cancer; Cell Biology; Immunology
 Applications Tested/Suitable for anti-ALPP antibody   

Immunofluorescence (IF), ELISA (EIA), Western Blot (WB)

 Application Notes for anti-ALPP antibody    IF~~1:100
WB~~1:500~3200
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 Immunofluorescence (IF) of anti-ALPP antibody    Fluorescent confocal image of HeLa cells stained with ALPP (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9200484 ALPP (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ALPP immunoreactivity is localized to the cytoplasm of HeLa cells.
anti-ALPP antibody Immunofluorescence (IF) image
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 Western Blot (WB) of anti-ALPP antibody    ALPP Antibody (N-term) western blot analysis in mouse placenta tissue lysates (35ug/lane).This demonstrates the ALPP antibody detected the ALPP protein (arrow).
anti-ALPP antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for ALPP. It may not necessarily be applicable to this product.
 NCBI GI #    94721246
 NCBI GeneID    250
 NCBI Accession #    NP_001623.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_001632.4 [Other Products]
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 UniProt Primary Accession #    P05187 [Other Products]
 UniProt Secondary Accession #    P05188; P06861; Q53S78; Q96DB7 [Other Products]
 UniProt Related Accession #    P05187 [Other Products]
 Molecular Weight    57954
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 NCBI Official Full Name    alkaline phosphatase, placental type preproprotein
 NCBI Official Synonym Full Names    alkaline phosphatase, placental
 NCBI Official Symbol    ALPP [Similar Products]
 NCBI Official Synonym Symbols   
ALP; PALP; PLAP; PLAP-1
[Similar Products]
 NCBI Protein Information    alkaline phosphatase, placental type
 UniProt Protein Name    Alkaline phosphatase, placental type
 UniProt Synonym Protein Names   
Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1
 Protein Family    Alkaline phosphatase
 UniProt Gene Name    ALPP [Similar Products]
 UniProt Synonym Gene Names    PLAP; PLAP-1 [Similar Products]
 UniProt Entry Name    PPB1_HUMAN
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 NCBI Summary for ALPP    The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]
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 UniProt Comments for ALPP    ALPP: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]

Protein type: Motility/polarity/chemotaxis; Phosphatase (non-protein); Cell surface; Membrane protein, integral; Cofactor and Vitamin Metabolism - folate biosynthesis; Membrane protein, GPI anchor; EC 3.1.3.1

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: cell surface; plasma membrane; integral to membrane

Molecular Function: zinc ion binding; alkaline phosphatase activity; magnesium ion binding

Biological Process: dephosphorylation
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Product References and Citations for anti-ALPP antibody

   References for protein:
1.Stec, B., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 8), 866-870 (2010)
2.Wang, F., et al. Am. J. Surg. Pathol. 33(10):1529-1539(2009)
3.Estrada, K., et al. Hum. Mol. Genet. 18(18):3516-3524(2009)
4.Zhu, J.F., et al. Zhonghua Wai Ke Za Zhi 47(5):381-384(2009)
5.Roberson, J.R., et al. Pediatr Blood Cancer 51(6):840-842(2008)

References for HeLa cell line:

1. Scherer WF, Syverton JT, Gey GO (May 1953). "Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix". J. Exp. Med. 97 (5): 695-710. [PubMed:13052828].

2. Macville M, Schröck E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T (January 1999). "Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping". Cancer Res. 59 (1): 141-50. [PubMed: 9892199].
3. Rahbari R, Sheahan T, Modes V, Collier P, Macfarlane C, Badge RM (April 2009). "A novel L1 retrotransposon marker for HeLa cell line identification". BioTechniques 46 (4): 277-84. [PubMed: 19450234].

4. Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (July 2010). "Check your cultures! A list of cross-contaminated or misidentified cell lines". Int. J. Cancer 127 (1): 1-8. [PubMed:20143388].
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 Research Articles on ALPP    1. term placental explants, but not their conditioned medium, can de-phosphorylate IGFBP-1 through the action of placental alkaline phosphatase
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ALPP antibodyPathways associated with anti-ALPP antibody
 Reference Product  PubMed Publications
 PTS antibody  >1 publications with ALPP and PTS
 Products by Pathway  Pathway Diagram
 NADH To Dimethyl Sulfoxide Electron Transfer Pathway antibodies  NADH To Dimethyl Sulfoxide Electron Transfer Pathway Diagram
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 NADH To Dimethyl Sulfoxide Electron Transfer Pathway antibodies  NADH To Dimethyl Sulfoxide Electron Transfer Pathway Diagram
 NADH To Dimethyl Sulfoxide Electron Transfer Pathway antibodies  NADH To Dimethyl Sulfoxide Electron Transfer Pathway Diagram
Diseases associated with anti-ALPP antibodyOrgans/Tissues associated with anti-ALPP antibody
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >28 publications with ALPP and Adenocarcinoma
 Nervous System Diseases Antibodies  >19 publications with ALPP and Nervous System Diseases
 Lung Diseases Antibodies  >12 publications with ALPP and Lung Diseases
 Breast Neoplasms Antibodies  >6 publications with ALPP and Breast Neoplasms
 Liver Diseases Antibodies  >6 publications with ALPP and Liver Diseases
 Disorders of Sex Development Antibodies  >5 publications with ALPP and Disorders of Sex Development
 Pain Antibodies  >4 publications with ALPP and Pain
 Kidney Diseases Antibodies  >4 publications with ALPP and Kidney Diseases
 Colonic Neoplasms Antibodies  >4 publications with ALPP and Colonic Neoplasms
 Necrosis Antibodies  >3 publications with ALPP and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Placenta Antibodies  >86 publications with ALPP and Placenta
 Intestine Antibodies  >10 publications with ALPP and Intestine
 Heart Antibodies  >8 publications with ALPP and Heart
 Uterus Antibodies  >7 publications with ALPP and Uterus
 Amniotic Fluid Antibodies  >1 publications with ALPP and Amniotic Fluid
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