Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow ADRBK1 rightarrow Monoclonal ADRBK1  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Annexin A2 (ANXA2) Recombinant Protein
 • IFNB1 Antibody
 • FMO3 cDNA Clone
 • Troponin I-Cardiac (148-158 Region) (TNNI3) Antibody
 • JAK2 Antibody
 • Goat Anti-Mouse kappa (kappa chain specific) Secondary Antibody
 • Troponin T-Cardiac (60-70 Region) (Tnnt2) Antibody
 • Bacillus anthracis Lethal Factor Antigen Antibody
 • D-LDH ELISA Kit
 • Androgen Receptor (AR) ELISA Kit
 • Catecholamine ELISA Kit
 • serine dehydratase (SDS) ELISA Kit
 • 6 Dihydroxy Melatonin ELISA Kit
 • Testosterone 19 Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-ADRBK1 antibody :: Mouse Adrenergic Receptor Kinase, beta 1 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS606296 anti-ADRBK1 antibody
Unit / Price
0.1 mg  /  $620 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Adrenergic Receptor Kinase, beta 1 (ADRBK1), Monoclonal Antibody

 Also Known As   

Adrenergic Receptor Kinase, beta 1 (bARK1, B1 AR, ARK1 beta, G Protein Coupled Receptor Kinase 2, GPCR K2, GRK2)

 Product Synonym Names    Anti -Adrenergic Receptor Kinase, beta 1 (bARK1, B1 AR, ARK1 beta, G Protein Coupled Receptor Kinase 2, GPCR K2, GRK2)
 Product Gene Name   

anti-ADRBK1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Chromosome Location    Chromosome: 11; NC_000011.9 (67033905..67054029). Location: 11q13.1
 OMIM    109635
 3D Structure    ModBase 3D Structure for P25098
Section Bar
 Clonality    Monoclonal
 Isotype    IgG2b
 Clone Number    3G3
 Host    Mouse
 Species Reactivity    Human, Mammal, Rat
Section Bar
 Specificity    Reacts with mammalian b-adrenergic receptor kinase.
 Purity/Purification    Affinity Purified
Purified by Protein A affinity chromatography.
 Form/Format    Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
Section Bar
 Immunogen    Recombinant b-adrenergic receptor kinase.
Section Bar
 Preparation and Storage    May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of anti-ADRBK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Product Description specifically for anti-ADRBK1 antibody

   G protein-coupled receptor kinases, a more recently discovered family, have the unique features of phosphorylating G-protein coupled receptors only when they are in their active or stimulated conformations. The range of signals acting through G protein-coupled receptors includes numerous neurotransmitters, chemoattractants, hormones, cytokines, and sensory stimuli such as photons or odorants. Structurally, the G-protein coupled receptor kinases show significant amino acid similarity to other kinases only in the centrally located catalytic domain of about 240 amino acid residues. Rapid phosphorylation of many G protein coupled receptors accompanies stimulus-driven desensitization. There are two types of kinases known to mediate these modifications: second messenger kinases and novel G protein-coupled receptor kinases. G protein-coupled receptor kinase family members include rhodopsin kinase, two isoforms of beta-adrenergic receptor kinase (betaARK), a gene cloned from the Huntington's disease locus on chromosome 4, GRK5, and several homologs from Drosophila. BetaARK1 is widely distributed throughout the mammalian central nervous system, especially at synapses, suggesting broader roles in regulating a wide variety of neurotransmitter receptors. BetaARK1 was identified as a kinase that phosphorylates betaARs in an agonist-dependent manner. Beta ARK1 has properties similar to the rhodopsin kinase in that BetaARK is also insensitive to cAMP, cGMP, and Ca2+ and does not phosphorylate histone. BetaARK is capable of phosphorylating photoexcited rhodopsin, although the betaAR is a better substrate for betaARK than rhodopsin. Both rhodopsin kinase and betaARK are inhibited by salts such as 0.1M NaCl, low concentrations of Zn (0.1-1mM), detergents such as digitonin, and polyanions such as dextransulfate and polyaspartic acid. Both kinases use ATP as the preferred substrate over GTP. In the case of betaARK, the kinase interacts with G protein beta-gamma subunits as well as with receptors, and the ternary complex of agonist bound receptor, kinase, and beta-gamma subunits is considered to be an active form for the phosphorylation of receptors. Multiple independent lines of evidence indicate that the phosphorylation of receptors by G-protein coupled receptor kinases in vivo regulates their function.
 Product Categories/Family for anti-ADRBK1 antibody    Antibodies; Abs to G Protein Receptors
 Applications Tested/Suitable for anti-ADRBK1 antibody   

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)

 Application Notes for anti-ADRBK1 antibody    Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Dilution: Immunofluorescence: 3-10ug/ml
ELISA: 0.1-1ug/ml
Western Blot: 1-5ug/ml
Immunohistochemistry: 10ug/ml
Immunoprecipitation: 10ug/IP reaction
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for ADRBK1. It may not necessarily be applicable to this product.
 NCBI GI #    23272739
 NCBI GeneID    156
Section Bar
 UniProt Primary Accession #    P25098 [Other Products]
 UniProt Secondary Accession #    Q13837; Q6GTT3; B0ZBE1 [Other Products]
 UniProt Related Accession #    P25098; Q59F73 [Other Products]
 Molecular Weight    79,574 Da [Similar Products]
Table BarTOPTable Bar
 NCBI Official Full Name    Adrenergic, beta, receptor kinase 1
 NCBI Official Synonym Full Names    adrenergic, beta, receptor kinase 1
 NCBI Official Symbol    ADRBK1 [Similar Products]
 NCBI Official Synonym Symbols   
GRK2; BARK1; FLJ16718; BETA-ARK1
[Similar Products]
 NCBI Protein Information    beta-adrenergic receptor kinase 1; beta-ARK-1; OTTHUMP00000235171; G-protein coupled receptor kinase 2
 UniProt Protein Name    Beta-adrenergic receptor kinase 1
 UniProt Synonym Protein Names   
G-protein coupled receptor kinase 2
 UniProt Gene Name    ADRBK1 [Similar Products]
 UniProt Synonym Gene Names    BARK; BARK1; GRK2 [Similar Products]
 UniProt Entry Name    ARBK1_HUMAN
Section Bar
 NCBI Summary for ADRBK1    The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq]
Section Bar
 UniProt Comments for ADRBK1    GRK2: a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function.

Protein type: Kinase, protein; EC 2.7.11.15; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; GRK family; BARK subfamily

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: G-protein coupled receptor kinase activity; protein binding; beta-adrenergic receptor kinase activity; Edg-2 lysophosphatidic acid receptor binding; alpha-2A adrenergic receptor binding; ATP binding; protein kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; desensitization of G-protein coupled receptor protein signaling pathway; tachykinin signaling pathway; fibroblast growth factor receptor signaling pathway; acetylcholine receptor signaling, muscarinic pathway; nerve growth factor receptor signaling pathway; heart development; peptidyl-threonine phosphorylation; signal transduction; negative regulation of striated muscle contraction; peptidyl-serine phosphorylation; negative regulation of the force of heart contraction by chemical signal; phospholipase C activation; receptor internalization; innate immune response; positive regulation of catecholamine secretion; cardiac muscle contraction
Table BarTOPTable Bar
 Research Articles on ADRBK1    1. GRK2 appears to play a significant role in regulating collagen synthesis in adult human CF, and increased activity of this kinase may be an important mechanism of maladaptive ventricular remodeling as mediated by cardiac fibroblasts
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-ADRBK1 antibodyPathways associated with anti-ADRBK1 antibody
 Reference Product  PubMed Publications
 SRC antibody  >24 publications with ADRBK1 and SRC
 ADRA2A antibody  >17 publications with ADRBK1 and ADRA2A
 MAPK3 antibody  >16 publications with ADRBK1 and MAPK3
 EGF antibody  >10 publications with ADRBK1 and EGF
 EGFR antibody  >7 publications with ADRBK1 and EGFR
 RHO antibody  >7 publications with ADRBK1 and RHO
 GIT1 antibody  >5 publications with ADRBK1 and GIT1
 PEBP1 antibody  >5 publications with ADRBK1 and PEBP1
 PRKCA antibody  >4 publications with ADRBK1 and PRKCA
 CCR4 antibody  >2 publications with ADRBK1 and CCR4
 Products by Pathway  Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Ca-dependent Events Pathway antibodies  Ca-dependent Events Pathway Diagram
 CaM Pathway antibodies  CaM Pathway Diagram
 Calmodulin Induced Events Pathway antibodies  Calmodulin Induced Events Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 G-protein Mediated Events Pathway antibodies  G-protein Mediated Events Pathway Diagram
Diseases associated with anti-ADRBK1 antibodyOrgans/Tissues associated with anti-ADRBK1 antibody
 Disease Name  Pubmed Publications
 Hypertension Antibodies  >31 publications with ADRBK1 and Hypertension
 Inflammation Antibodies  >27 publications with ADRBK1 and Inflammation
 Neoplasms Antibodies  >21 publications with ADRBK1 and Neoplasms
 Hypertrophy Antibodies  >17 publications with ADRBK1 and Hypertrophy
 Pain Antibodies  >15 publications with ADRBK1 and Pain
 Neurodegenerative Diseases Antibodies  >10 publications with ADRBK1 and Neurodegenerative Diseases
 Cardiomegaly Antibodies  >10 publications with ADRBK1 and Cardiomegaly
 Necrosis Antibodies  >10 publications with ADRBK1 and Necrosis
 Genital Diseases, Male Antibodies  >7 publications with ADRBK1 and Genital Diseases, Male
 Hyperalgesia Antibodies  >7 publications with ADRBK1 and Hyperalgesia
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >83 publications with ADRBK1 and Muscle
 Blood Antibodies  >72 publications with ADRBK1 and Blood
 Brain Antibodies  >69 publications with ADRBK1 and Brain
 Kidney Antibodies  >53 publications with ADRBK1 and Kidney
 Vascular Antibodies  >35 publications with ADRBK1 and Vascular
 Nerve Antibodies  >17 publications with ADRBK1 and Nerve
 Ovary Antibodies  >10 publications with ADRBK1 and Ovary
 Lung Antibodies  >9 publications with ADRBK1 and Lung
 Eye Antibodies  >9 publications with ADRBK1 and Eye
 Bone Antibodies  >8 publications with ADRBK1 and Bone
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions