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Mouse anti-Human Aggrecan G1-IGD-G2 Domains Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS608516 anti-Aggrecan G1-IGD-G2 Domains antibody
Unit / Price
0.5 mg  /  $760 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Aggrecan G1-IGD-G2 Domains, Monoclonal Antibody

 Also Known As   

Aggrecan G1-IGD-G2 Domains (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, M

 Product Synonym Names    Anti -Aggrecan G1-IGD-G2 Domains (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, M
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG2b
 Clone Number    9H46
 Host    Mouse
 Species Reactivity    Human
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 Specificity    Recognizes human Aggrecan G1-IGD-G2 Domains.
 Purity/Purification    Affinity Purified
Purified by Protein G affinity chromatography.
 Form/Format    Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
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 Immunogen    Recombinant corresponding to aa20-675 from human Aggrecan G1-IGD-G2 Domains, expressed in NS0 cells (NP_037359).
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 Preparation and Storage    Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of anti-Aggrecan G1-IGD-G2 Domains antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Aggrecan G1-IGD-G2 Domains antibody

   As the key component of the cartilage extracellular matrix, aggrecan hydrates the collagen network and provides cartilage with its properties of compressibility and elasticity. Maintenance of aggrecan content is therefore critical to the function of the tissue and aggrecan degradation is an important factor in the erosion of articular cartilage in arthritic diseases. The deduced amino acid sequence of human aggrecan core protein consists of 2415 residues and predicts a signal peptide and domains of G1, IGD, G2, KS, CS­1, CS­2 and G3 (3). Two globular domains, G1 and G2, comprise the N­terminus of the proteoglycan and also contain link domains. The third globular domain, G3, corresponds to the C­terminus. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains are between G2 and G3. With KS and CS attached to the 250kD core protein, aggrecan monomers exist as a 1000-2000kD molecule.
 Product Categories/Family for anti-Aggrecan G1-IGD-G2 Domains antibody    Antibodies; Abs to Carbohydrates, Glycoproteins
 Applications Tested/Suitable for anti-Aggrecan G1-IGD-G2 Domains antibody   

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

 Application Notes for anti-Aggrecan G1-IGD-G2 Domains antibody    Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry.
Dilution: Direct ELISA: 0.5-1.0ug/ml the detection limit is ~2ng/well
Western Blot: 1-2ug/ml the detection limit is ~5ng/lane under non-reducing conditions
Immunohistochemistry: 25ug/ml detects aggrecan in paraffin-embedded human cartilage tissue sections.
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human aggrecan from conditioned media of transfected NSO cells. Dectection using A1059-53H.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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