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anti-ACAN antibody :: Mouse Aggrecan, N-terminal neoepitope FFGV Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS442016
Product Data
Unit / Price
0.1 mg  /  $435 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Aggrecan, N-terminal neoepitope FFGV (ACAN), Monoclonal Antibody

 Also Known As   

Monocloncal Antibody FFGV (Clone BC14)

 Product Synonym Names    Aggrecan Antibody, N-terminal neoepitope FFGV
 Product Gene Name   

anti-ACAN antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 15; NC_000015.10 (88803442..88875354). Location: 15q26.1
 OMIM    155760
 3D Structure    ModBase 3D Structure for P16112
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 Clonality    Monoclonal
 Isotype    IgG2a
 Clone Number    BC-14
 Host    Mouse
 Species Reactivity    Human, rat, guinea pig, horse, pig
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 Specificity    Recognizes the N-terminal neoepitope sequence (FFGV....) generated at the “MMP cleavage site” after MMP catabolism in the interglobular domain of aggrecan between amino acids ..PEN341 and 342FFG.. (Human sequence enumeration).
 Purity/Purification    Affinity purified on protein G
 Form/Format    Liquid, 1 mL/vial
 Concentration    0.1 mg/mL (lot specific)
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 Immunogen    Synthetic peptide: FFGVGGE
 Myeloma    x63-Ag8.653
 Light Chain Type    kappa
 Technical Notes    This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections. Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry.
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 Preparation and Storage    Store at -20 degree C.
 Other Notes    Small volumes of anti-ACAN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-ACAN antibody

   Aggrecan monoclonal antibody to N-terminal neoepitope FFGV (mouse, clone BC14). This fragment is rapidly released from the tissue when MMP catabolism of aggrecan occurs and has been identified in synovial fluid samples from patients with degenerative joint diseases.

Overview: Proteoglycans are categoried depending upon the nature of their glycosaminoglycan chains (chondroitin sulfate, dermatan sulfate, heparan sulphate and keratan sullfate) as well as characterized by size. Aggrecan is a large aggregating proteoglycan of articular cartilage. It is found also in aorta tissue, discs, tendons and in the perineuronal net. It is responsible for hydrating cartilage, giving it compressibility and resiliance during joint loading, thereby playing a major role in the normal function of cartilage. Depletion of glycosaminoglycan bearing aggrecan fragments is one of the earliest events in cartilage destruction. A) Aggrecan monomer showing proteolytic cleavage sites B) Aggregate showing aggrecan monomers bound to hyaluronic acid
 Product Categories/Family for anti-ACAN antibody    Antibodies to Aggrecan
 Applications Tested/Suitable for anti-ACAN antibody   

Western Blot (WB)

 Application Notes for anti-ACAN antibody    Western-Blotting - Suggested dilution: 1:100. Detects a a band of approximately 60 - 150 kDa; ELISA; IHC
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 Product Data of anti-ACAN antibody    anti-ACAN antibody Product Data image
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NCBI/Uniprot data below describe general gene information for ACAN. It may not necessarily be applicable to this product.
 NCBI GI #    22209083
 NCBI GeneID    176
 NCBI Accession #    AAH36445.1 [Other Products]
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 UniProt Primary Accession #    P16112 [Other Products]
 UniProt Secondary Accession #    Q13650; Q9UCD3; Q9UCP4; Q9UCP5; Q9UDE0 [Other Products]
 UniProt Related Accession #    P16112 [Other Products]
 Molecular Weight    250,193 Da [Similar Products]
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 NCBI Official Full Name    Aggrecan
 NCBI Official Synonym Full Names    aggrecan
 NCBI Official Symbol    ACAN [Similar Products]
 NCBI Official Synonym Symbols   
AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP
[Similar Products]
 NCBI Protein Information    aggrecan core protein; large aggregating proteoglycan; cartilage-specific proteoglycan core protein; chondroitin sulfate proteoglycan core protein 1
 UniProt Protein Name    Aggrecan core protein
 UniProt Synonym Protein Names   
Cartilage-specific proteoglycan core protein; CSPCP; Chondroitin sulfate proteoglycan core protein 1
 Protein Family    Aggrecan core protein
 UniProt Gene Name    ACAN [Similar Products]
 UniProt Synonym Gene Names    AGC1; CSPG1; MSK16; CSPCP [Similar Products]
 UniProt Entry Name    PGCA_HUMAN
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 NCBI Summary for ACAN    This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ACAN    Function: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

Subunit structure: Interacts with FBLN1

By similarity. Interacts with COMP. Ref.11

Subcellular location: Secreted › extracellular space › extracellular matrix

By similarity.

Tissue specificity: Restricted to cartilages. Ref.4

Developmental stage: Expression was detected in chondrocytes throughout the developing skeleton.

Domain: Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.

Post-translational modification: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.

Involvement in disease: Spondyloepiphyseal dysplasia type Kimberley (SEDK) [MIM:608361]: Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.9Spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN) [MIM:612813]: A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts.Note: The disease is caused by mutations affecting the gene represented in this entry.Osteochondritis dissecans short stature and early-onset osteoarthritis (OD) [MIM:165800]: A type of osteochondritis defined as a separation of cartilage and subchondral bone from the surrounding tissue, primarily affecting the knee, ankle and elbow joints. It is clinically characterized by multiple osteochondritic lesions in knees and/or hips and/or elbows, disproportionate short stature and early-onset osteoarthritis.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.13

Sequence similarities: Belongs to the aggrecan/versican proteoglycan family.Contains 1 C-type lectin domain.Contains 1 EGF-like domain.Contains 1 Ig-like V-type (immunoglobulin-like) domain.Contains 4 Link domains.Contains 1 Sushi (CCP/SCR) domain.
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 Research Articles on ACAN    1. the subsequent presentation of aggregan from ECM leads to CD4(+) T-cell activation and effector cell formation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ACAN antibodyPathways associated with anti-ACAN antibody
 Reference Product  PubMed Publications
 SOX9 antibody  >49 publications with ACAN and SOX9
 ADAMTS4 antibody  >8 publications with ACAN and ADAMTS4
 BMP2 antibody  >6 publications with ACAN and BMP2
 FMOD antibody  >4 publications with ACAN and FMOD
 LUM antibody  >3 publications with ACAN and LUM
 KERA antibody  >1 publications with ACAN and KERA
 Products by Pathway  Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
 Keratan Sulfate Biosynthesis Pathway antibodies  Keratan Sulfate Biosynthesis Pathway Diagram
 Keratan Sulfate Degradation Pathway antibodies  Keratan Sulfate Degradation Pathway Diagram
 Keratan Sulfate/keratin Metabolism Pathway antibodies  Keratan Sulfate/keratin Metabolism Pathway Diagram
 MPS I - Hurler Syndrome Pathway antibodies  MPS I - Hurler Syndrome Pathway Diagram
Organs/Tissues associated with anti-ACAN antibody
 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >24 publications with ACAN and Connective Tissue
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