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anti-Akt1 antibody :: Rabbit Akt Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS633224 anti-Akt1 antibody
Unit / Price
0.1 mL  /  $505 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Akt (Akt1), Monoclonal Antibody

 Also Known As   

Akt, phosphorylated (Ser473) (Rac PKa, PKBa)

 Product Synonym Names    Anti -Akt, phosphorylated (Ser473) (Rac PKa, PKBa)
 Product Gene Name   

anti-Akt1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 3R; NT_033777.2 (11924938..11930239). Location: 89B3-89B3
 3D Structure    ModBase 3D Structure for Q8INB9
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 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    10g137
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    Recognizes endogenous levels of Akt only when phosphorylated at Ser473. Species crossreactivity: Mouse and rat.
 Purity/Purification    Supernatant
Supernatant
 Form/Format    Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.
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 Immunogen    Synthetic phosphopeptide corresponding to residues around Ser473 of mouse Akt.
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 Preparation and Storage    May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
 Other Notes    Small volumes of anti-Akt1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-Akt1 antibody

   Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9) and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3alpha and beta (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3beta mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18). Inhibition of mTOR stops the protein synthesis machinery by inactivating p70 S6 kinase and activating the eukaryotic initiation factor 4E binding protein 1 (4E-BP1), an inhibitor of translation (18,19).
 Product Categories/Family for anti-Akt1 antibody    Antibodies; Abs to Protein Kinases
 Applications Tested/Suitable for anti-Akt1 antibody   

Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF)

 Application Notes for anti-Akt1 antibody    Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunoprecipitation.
Dilution: Immunofluorescence (IF-IC): 1:200
Flow Cytometry: 1:400
Western Blot: 1:1000
Immunoprecipitation: 1:200
Optimal dilutions to be determined by the researcher
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NCBI/Uniprot data below describe general gene information for Akt1. It may not necessarily be applicable to this product.
 NCBI GI #    24647360
 NCBI GeneID    41957
 NCBI Accession #    NP_732115.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_169707.1 [Other Products]
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 UniProt Primary Accession #    Q8INB9 [Other Products]
 UniProt Secondary Accession #    Q0KI65; Q24293; Q24469; Q24470; Q7JN11; Q8T9A5; Q9VEY7 [Other Products]
 UniProt Related Accession #    Q24469 [Other Products]
 Molecular Weight    68,485 Da [Similar Products]
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 NCBI Official Full Name    Akt1, isoform B
 NCBI Official Symbol    Akt1 [Similar Products]
 NCBI Official Synonym Symbols   
akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq;
[Similar Products]
 NCBI Protein Information    CG4006 gene product from transcript CG4006-RA; Akt kinase; Akt1; Akt1-PA; Akt1-PB; Akt1-PC; CG4006-PA; CG4006-PB; CG4006-PC; dAkt kinase; protein kinase B; related to PKA to PKC protein kinases; related to the A and C kinases
 UniProt Protein Name    RAC serine/threonine-protein kinase
 UniProt Synonym Protein Names   
Akt; Protein kinase B
 Protein Family    AKT-interacting protein
 UniProt Gene Name    Akt1 [Similar Products]
 UniProt Synonym Gene Names    DAkt; DRAC-PK; Dakt1; PKB [Similar Products]
 UniProt Entry Name    AKT1_DROME
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 UniProt Comments for Akt1    Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Oncoprotein; AGC group; AKT family

Cellular Component: cell surface; cell soma; cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; ATP binding; phosphoinositide binding; protein kinase activity

Biological Process: oogenesis; circadian rhythm; positive regulation of cell size; chitin-based embryonic cuticle biosynthetic process; regulation of dendrite development; positive regulation of multicellular organism growth; protein amino acid phosphorylation; somatic muscle development; regulation of protein import into nucleus; regulation of cell shape; sequestering of lipid; myoblast fusion; negative regulation of synaptic growth at neuromuscular junction; positive regulation of axon regeneration; multicellular organism growth; regulation of multicellular organism growth; regulation of organ growth; epithelial cell migration, open tracheal system; positive regulation of organ growth; positive regulation of cell growth; dendrite regeneration; regulation of hemocyte proliferation; open tracheal system development; insulin receptor signaling pathway; lipid metabolic process; response to oxidative stress; regulation of cell size; negative regulation of apoptosis
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 Research Articles on Akt1    1. Hippo signaling not only blocks cell division and promotes apoptosis, but also regulates cellular growth by inhibiting the Akt pathway activity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-Akt1 antibodyDiseases associated with anti-Akt1 antibody
 Products by Pathway  Pathway Diagram
 AKT Phosphorylates Targets In The Cytosol Pathway antibodies  AKT Phosphorylates Targets In The Cytosol Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 AKT-mediated Inactivation Of FOXO1A Pathway antibodies  AKT-mediated Inactivation Of FOXO1A Pathway Diagram
 Activation Of PKB Pathway antibodies  Activation Of PKB Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram
 CTLA4 Inhibitory Signaling Pathway antibodies  CTLA4 Inhibitory Signaling Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1891 publications with Akt1 and Neoplasms
 Breast Neoplasms Antibodies  >362 publications with Akt1 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >317 publications with Akt1 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >278 publications with Akt1 and Nervous System Diseases
 Necrosis Antibodies  >276 publications with Akt1 and Necrosis
 Lung Neoplasms Antibodies  >198 publications with Akt1 and Lung Neoplasms
 Prostatic Neoplasms Antibodies  >184 publications with Akt1 and Prostatic Neoplasms
 Neoplasms, Experimental Antibodies  >175 publications with Akt1 and Neoplasms, Experimental
 Inflammation Antibodies  >154 publications with Akt1 and Inflammation
 Heart Diseases Antibodies  >137 publications with Akt1 and Heart Diseases
Organs/Tissues associated with anti-Akt1 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >732 publications with Akt1 and Blood
 Embryonic Tissue Antibodies  >124 publications with Akt1 and Embryonic Tissue
 Head Antibodies  >75 publications with Akt1 and Head
 Ovary Antibodies  >58 publications with Akt1 and Ovary
 Fat Body Antibodies  >29 publications with Akt1 and Fat Body
 Whole Body Antibodies  >28 publications with Akt1 and Whole Body
 Uncharacterized Tissue Antibodies  >3 publications with Akt1 and Uncharacterized Tissue
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