Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow AKT1 rightarrow Monoclonal AKT1  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Procollagen C-Endopeptidase Enhancer 1 (PCOLCE1) ELISA Kit
 • ATP1A2 Antibody
 • Rotavirus Antibody
 • ACAD8 Antibody
 • Tumour necrosis factor related weak inducer of apoptosis (TW... ELISA Kit
 • TOCA-1 (FNBP1L) Antibody
 • TSPO-PBR Antibody
 • Mucin 5 Subtype AC (MUC5AC) Recombinant Protein
 • HE4 Antibody
 • Protein phosphatase 1 regulatory subunit 11 (PPP1R11) Antibody
 • Amiloride-sensitive sodium channel subunit alpha (SCNN1A) ELISA Kit
 • KLK3 - PSA Antibody
 • ERAP2 Antibody
 • Tumor protein p73 (TP73) Recombinant Protein
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-AKT1 antibody :: Mouse Akt1, PH Domain, Serine-Threonine Phosphorylation Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS601744 anti-AKT1 antibody
Unit / Price
0.1 mg  /  $645 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Akt1, PH Domain, Serine-Threonine Phosphorylation, Monoclonal Antibody

 Also Known As   

Akt1, PH Domain, Serine-Threonine Phosphorylation (Rac PKa, PKBa)

 Product Synonym Names    Anti -Akt1, PH Domain, Serine-Threonine Phosphorylation (Rac PKa, PKBa)
 Product Gene Name   

anti-AKT1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Chromosome Location    Chromosome: 14; NC_000014.8 (105235686..105262080, complement). Location: 14q32.32; 14q32.32
 OMIM    114480
 3D Structure    ModBase 3D Structure for P31749
Section Bar
 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    1.T.1
 Host    Mouse
 Species Reactivity    Human, Mouse, Rat
Section Bar
 Specificity    Recognizes human Akt1/PKBa > Akt2/PKBb (30%) > Akt3/PKBg (weak to none) based on Western blot analysis using 100ng recombinant fusion protein for each isoform.
 Purity/Purification    Affinity Purified
Purified by Protein G affinity chromatography.
 Form/Format    Supplied as a liquid in PBS, 0.05% sodium azide.
Section Bar
 Immunogen    GST-fusion protein corresponding to residues 1-149 of human Akt1
Section Bar
 Preparation and Storage    May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
 Other Notes    Small volumes of anti-AKT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 Product Categories/Family for anti-AKT1 antibody    Antibodies; Abs to Protein Kinases
 Applications Tested/Suitable for anti-AKT1 antibody   

Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

 Application Notes for anti-AKT1 antibody    Suitable for use in Flow Cytometry, Western Blot, Immunocytochemistry, Immunoprecipitation Kinase and Immunoprecipitation.
Dilution: Western Blot Analysis: 0.1-1ug/ml detects Akt (60kD) in RIPA lysates from A431 cells. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A1125-04 (0.5 and 1.0ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitates Akt from 500ug of L6 RIPA lysate.
Immunoprecipitation Kinase: 4ug immunoprecipitates active Akt from 700ug of L6 cells stimulated with 15nM IGF-1 for 2-5 min.
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for AKT1. It may not necessarily be applicable to this product.
 NCBI GI #    18027298
 NCBI GeneID    207
Section Bar
 UniProt Primary Accession #    P31749 [Other Products]
 UniProt Secondary Accession #    Q9BWB6; B2RAM5 [Other Products]
 UniProt Related Accession #    P31749; Q9BV07 [Other Products]
 Molecular Weight    55,686 Da [Similar Products]
Table BarTOPTable Bar
 NCBI Official Full Name    AKT1
 NCBI Official Synonym Full Names    v-akt murine thymoma viral oncogene homolog 1
 NCBI Official Symbol    AKT1 [Similar Products]
 NCBI Official Synonym Symbols   
AKT; PKB; RAC; PRKBA; MGC99656; PKB-ALPHA; RAC-ALPHA
[Similar Products]
 NCBI Protein Information    RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; protein kinase B; proto-oncogene c-Akt; rac protein kinase alpha
 UniProt Protein Name    RAC-alpha serine/threonine-protein kinase
 UniProt Synonym Protein Names   
Protein kinase B; PKB; Proto-oncogene c-Akt; RAC-PK-alpha
 Protein Family    Potassium channel
 UniProt Gene Name    AKT1 [Similar Products]
 UniProt Synonym Gene Names    PKB; RAC [Similar Products]
 UniProt Entry Name    AKT1_HUMAN
Section Bar
 NCBI Summary for AKT1    The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]
Section Bar
 UniProt Comments for AKT1    Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: Oncoprotein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.1; Kinase, protein; AGC group; AKT family

Chromosomal Location of Human Ortholog: 14q32.32

Cellular Component: microtubule cytoskeleton; nucleoplasm; mitochondrion; cytoplasm; plasma membrane; spindle; intercellular junction; nucleus; cytosol

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; enzyme binding; phosphatidylinositol-3,4,5-triphosphate binding; protein kinase C binding; nitric-oxide synthase regulator activity; protein serine/threonine/tyrosine kinase activity; kinase activity; phosphatidylinositol-3,4-bisphosphate binding; ATP binding; protein kinase activity

Biological Process: negative regulation of JNK cascade; regulation of myelination; positive regulation of nitric oxide biosynthetic process; nerve growth factor receptor signaling pathway; protein ubiquitination; glucose homeostasis; regulation of cell migration; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; protein amino acid phosphorylation; germ cell development; positive regulation of glucose import; cell projection organization and biogenesis; protein catabolic process; maternal placenta development; response to food; platelet activation; glycogen biosynthetic process; fibroblast growth factor receptor signaling pathway; positive regulation of nitric-oxide synthase activity; positive regulation of blood vessel endothelial cell migration; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; response to heat; T cell costimulation; negative regulation of protein kinase activity; striated muscle cell differentiation; positive regulation of fat cell differentiation; positive regulation of transcription factor activity; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of nitric-oxide synthase activity; response to oxidative stress; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of fatty acid beta-oxidation; translation; apoptosis; protein amino acid autophosphorylation; positive regulation of glycogen biosynthetic process; regulation of glycogen biosynthetic process; positive regulation of cellular protein metabolic process; negative regulation of cell size; negative regulation of caspase activity; glucose transport; signal transduction; nitric oxide metabolic process; regulation of translation; apoptotic mitochondrial changes; protein kinase B signaling cascade; nitric oxide biosynthetic process; cell differentiation; inflammatory response; activated T cell apoptosis; aging; epidermal growth factor receptor signaling pathway; negative regulation of proteolysis; phosphoinositide-mediated signaling; myelin maintenance in the peripheral nervous system; protein modification process; positive regulation of peptidyl-serine phosphorylation; endocrine pancreas development; osteoblast differentiation; cell proliferation; G-protein coupled receptor protein signaling pathway; peptidyl-serine phosphorylation; protein import into nucleus, translocation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; insulin receptor signaling pathway; positive regulation of vasoconstriction; innate immune response; gene expression; positive regulation of protein amino acid phosphorylation; blood coagulation; vascular endothelial growth factor receptor signaling pathway; hyaluronan metabolic process; phosphorylation

Disease: Schizophrenia; Cowden Syndrome 6; Proteus Syndrome; Breast Cancer; Ovarian Cancer
Table BarTOPTable Bar
 Research Articles on AKT1    1. Data show that exposure of postmitotic neurons to caffeine leads to changes in tau phosphorylation concomitantly with downregulation of Akt signaling.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-AKT1 antibodyPathways associated with anti-AKT1 antibody
 Reference Product  PubMed Publications
 PTEN antibody  >408 publications with AKT1 and PTEN
 AKT2 antibody  >405 publications with AKT1 and AKT2
 CASP3 antibody  >329 publications with AKT1 and CASP3
 MTOR antibody  >286 publications with AKT1 and MTOR
 BAD antibody  >236 publications with AKT1 and BAD
 SRC antibody  >227 publications with AKT1 and SRC
 IRS1 antibody  >165 publications with AKT1 and IRS1
 CASP9 antibody  >119 publications with AKT1 and CASP9
 NOS3 antibody  >110 publications with AKT1 and NOS3
 FOXO1 antibody  >109 publications with AKT1 and FOXO1
 Products by Pathway  Pathway Diagram
 AKT Phosphorylates Targets In The Cytosol Pathway antibodies  AKT Phosphorylates Targets In The Cytosol Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 AKT-mediated Inactivation Of FOXO1A Pathway antibodies  AKT-mediated Inactivation Of FOXO1A Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
Diseases associated with anti-AKT1 antibodyOrgans/Tissues associated with anti-AKT1 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1637 publications with AKT1 and Neoplasms
 Breast Neoplasms Antibodies  >307 publications with AKT1 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >269 publications with AKT1 and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >237 publications with AKT1 and Adenocarcinoma
 Leukemia Antibodies  >209 publications with AKT1 and Leukemia
 Insulin Resistance Antibodies  >194 publications with AKT1 and Insulin Resistance
 Prostatic Neoplasms Antibodies  >170 publications with AKT1 and Prostatic Neoplasms
 Ischemia Antibodies  >161 publications with AKT1 and Ischemia
 Lung Neoplasms Antibodies  >160 publications with AKT1 and Lung Neoplasms
 Lung Diseases Antibodies  >156 publications with AKT1 and Lung Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >623 publications with AKT1 and Blood
 Vascular Antibodies  >569 publications with AKT1 and Vascular
 Muscle Antibodies  >569 publications with AKT1 and Muscle
 Brain Antibodies  >386 publications with AKT1 and Brain
 Lung Antibodies  >252 publications with AKT1 and Lung
 Heart Antibodies  >229 publications with AKT1 and Heart
 Nerve Antibodies  >218 publications with AKT1 and Nerve
 Prostate Antibodies  >204 publications with AKT1 and Prostate
 Liver Antibodies  >195 publications with AKT1 and Liver
 Kidney Antibodies  >140 publications with AKT1 and Kidney
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions