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anti-APAF1 antibody :: Mouse Apaf-1 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS150040
Unit / Price
0.1 mg  /  $365 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Apaf-1 (APAF1), Monoclonal Antibody

 Also Known As   

Apaf-1 Antibody [2E10]

 Product Synonym Names    Apaf-1 Antibody [2E10] : CED4; APAF-1; apoptotic peptidase activating factor 1
 Product Gene Name   

anti-APAF1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    602233
 3D Structure    ModBase 3D Structure for O14727
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 Clonality    Monoclonal
 Clone Number    2E10
 Host    Mouse
 Species Reactivity    Human, Mouse, Rat
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 Purity/Purification    Apaf-1 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
 Form/Format    Liquid
 Concentration    1 mg/mL (lot specific)
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 Conjugate    Unconjugated
 Immunogen    A peptide corresponding to amino acids near the carboxy terminus of human Apaf1.
 Buffer    Apaf-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
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 Preparation and Storage    Apaf-1 monoclonal antibody can be stored at -20 degree C, stable for one year.
 Other Notes    Small volumes of anti-APAF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-APAF1 antibody

   Apaf-1 Monoclonal Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.
 Applications Tested/Suitable for anti-APAF1 antibody   

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-APAF1 antibody    Apaf1 antibody can be used for detection of Apaf1 by Western blot at 0.25 - 0.5 mg/mL. A 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 0.5 mug/mL.
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 Western Blot (WB) of anti-APAF1 antibody    Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 0.25 and (B) 0.5 mg/mL.
anti-APAF1 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-APAF1 antibody    Immunohistochemistry of Apaf1 in K562 cells with Apaf1 antibody at 0.5 μg/mL.
anti-APAF1 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for APAF1. It may not necessarily be applicable to this product.
 NCBI GI #    2330015
 NCBI GeneID    317
 NCBI Accession #    AAC51678 [Other Products]
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 UniProt Primary Accession #    O14727 [Other Products]
 UniProt Secondary Accession #    O43297; Q7Z438; Q9BXZ6; Q9UBZ5; Q9UGN8; Q9UGN9; Q9UGP0; Q9UJ58; Q9UJ59; Q9UJ60; B2RMX8 [Other Products]
 UniProt Related Accession #    O14727 [Other Products]
 Molecular Weight    130 kDa
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 NCBI Official Full Name    apoptotic protease activating factor 1
 NCBI Official Synonym Full Names    apoptotic peptidase activating factor 1
 NCBI Official Symbol    APAF1 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    apoptotic protease-activating factor 1
 UniProt Protein Name    Apoptotic protease-activating factor 1
 Protein Family    Apoptotic protease-activating factor
 UniProt Gene Name    APAF1 [Similar Products]
 UniProt Synonym Gene Names    KIAA0413; APAF-1 [Similar Products]
 UniProt Entry Name    APAF_HUMAN
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 NCBI Summary for APAF1    This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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 UniProt Comments for APAF1    APAF: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). By E2F and p53/TP53 in apoptotic neurons. Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 12q23

Cellular Component: nucleus; cytosol; apoptosome

Molecular Function: identical protein binding; protein binding; nucleotide binding; caspase activator activity; ADP binding; ATP binding

Biological Process: caspase activation; regulation of apoptosis; nervous system development; neuron apoptosis; positive regulation of apoptosis; apoptosis; forebrain development; neural tube closure; caspase activation via cytochrome c
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 Research Articles on APAF1    1. IL-24 has a role in sensitizing melanoma cells to erlotinib through modulation of the Apaf-1 and Akt signaling pathways
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-APAF1 antibodyDiseases associated with anti-APAF1 antibody
 Products by Pathway  Pathway Diagram
 Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway antibodies  Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >90 publications with APAF1 and Necrosis
 Nervous System Diseases Antibodies  >78 publications with APAF1 and Nervous System Diseases
 Disease Models, Animal Antibodies  >27 publications with APAF1 and Disease Models, Animal
 Breast Neoplasms Antibodies  >25 publications with APAF1 and Breast Neoplasms
 Inflammation Antibodies  >22 publications with APAF1 and Inflammation
 Liver Diseases Antibodies  >21 publications with APAF1 and Liver Diseases
 Colonic Neoplasms Antibodies  >17 publications with APAF1 and Colonic Neoplasms
 Neoplasms, Experimental Antibodies  >17 publications with APAF1 and Neoplasms, Experimental
 Liver Neoplasms Antibodies  >17 publications with APAF1 and Liver Neoplasms
 Nerve Degeneration Antibodies  >16 publications with APAF1 and Nerve Degeneration
Organs/Tissues associated with anti-APAF1 antibody
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >102 publications with APAF1 and Brain
 Blood Antibodies  >77 publications with APAF1 and Blood
 Muscle Antibodies  >47 publications with APAF1 and Muscle
 Liver Antibodies  >45 publications with APAF1 and Liver
 Heart Antibodies  >36 publications with APAF1 and Heart
 Embryonic Tissue Antibodies  >29 publications with APAF1 and Embryonic Tissue
 Kidney Antibodies  >28 publications with APAF1 and Kidney
 Eye Antibodies  >26 publications with APAF1 and Eye
 Skin Antibodies  >26 publications with APAF1 and Skin
 Testis Antibodies  >14 publications with APAF1 and Testis
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