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anti-B7-H4 antibody :: Mouse anti-Human B7-H4 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS668282
Unit / Price
0.025 mg  /  $140 +1 FREE 8GB USB
0.1 mg  /  $260 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

B7-H4, Monoclonal Antibody

★Popular Item★
 Also Known As   

Monoclonal Antibody to B7-H4 (Clone: ABM53A6)

 Product Synonym Names    V-set domain-containing T-cell activation inhibitor 1; B7 homolog 4; B7h.5; Immune costimulatory protein B7-H4; Protein B7S1; Monoclonal antibody to B7H4 (Clone: ABM53A6)
 Product Gene Name   

anti-B7-H4 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    AY280972 mRNA
 3D Structure    ModBase 3D Structure for Q7Z7D3
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 Clonality    Monoclonal
 Isotype    IgG2a k
 Clone Number    ABM53A6
 Host    Mouse
 Species Reactivity    Human
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 Purity/Purification    Purified
Protein G Chromatography
 Form/Format    25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
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 Immunogen Information    A partial length recombinant protein (a.a 23-220) of B7-H4 was used as the immunogen for this antibody.
 Antigen    B7H4
 Conjugate    Unconjugated
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 Preparation and Storage    Store the antibody at 4C, stable for 6 months.
For long-term storage, store at -20C.
Avoid repeated freeze and thaw cycles.
 Other Notes    Small volumes of anti-B7-H4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-B7-H4 antibody

   B7-H4, a member of B7 family, is a transmembrane protein that has been shown to inhibit T cell responses and neutrophil expansion during bacterial infections. B7-H4 mRNA is widely distributed in mouse and human peripheral tissues and can be induced in monocytes, macrophages, and dendritic cells upon IL-6 and IL-10 stimulation. However, in a variety of tumor cells, B7-H4 is predominantly present in intracellular compartments with unknown mechanism and functions. Cell surface expression of B7-H4 protein is limited and shows an inducible pattern on hematopoietic cells. The overexpression of B7-H4 has been found in various types of human tumors, such as breast cancer, renal cell carcinoma (RCC), ovarian cancer, esophageal squamous cell carcinoma, gastric cancer, pancreatic cancer and melanoma etc, where its expression level is positively correlated with disease progression. By arresting cell cycle, B7-H4 ligation of T cells has a profound inhibitory effect on the growth, cytokine secretion, and development of cytotoxicity.
 Product Categories/Family for anti-B7-H4 antibody    Antibodies; Immunology
 Applications Tested/Suitable for anti-B7-H4 antibody   

Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)

 Application Notes for anti-B7-H4 antibody    FACS: 0.5-1 ug/10^6 cells
IHC: 5 ug/ml
WB: 2-4 ug/ml
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 Immunohistochemistry (IHC) of anti-B7-H4 antibody    Fig-1: Immunohistochemical analysis of B7-H4 antibody in Renal cell carcinoma using anti-B7-H4 antibody (Clone: ABM53A6) at 5 ug/ml
anti-B7-H4 antibody Immunohistochemistry (IHC) (IHC) image
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 Flow Cytometry (FC/FACS) of anti-B7-H4 antibody    Fig-2: Cell surface Flow analysis of B7-H4 antibody in human PBMC (Monocytes) cells using 0.5 ?g/ 10^6 cells of anti-B7-H4 antibody (ABM53A6). Green represents isotype control;red represents anti-B7H4 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
anti-B7-H4 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
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 Western Blot (WB) of anti-B7-H4 antibody    Fig-3: Western blot analysis of B7H4. Anti-B7H4 antibody (Clone: ABM53A6) was used at 4 ug/ml on human B7H4-FC fusion protein Lysate.
anti-B7-H4 antibody Western Blot (WB) (WB) image
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 Western Blot(WB)Fig-4: Western blot analysis of B7-H4. Anti-B7-H4 antibody (Clone: ABM53A6) was used at 2 g/ml on (1) HCT-116, (2) PC3, (3) Kato 111, (4) C2C12 and (5) RAW Lysates. of anti-B7-H4 antibody    anti-B7-H4 antibody Western Blot(WB)Fig-4: Western blot analysis of B7-H4. Anti-B7-H4 antibody (Clone: ABM53A6) was used at 2 g/ml on (1) HCT-116, (2) PC3, (3) Kato 111, (4) C2C12 and (5) RAW Lysates. (WB) image
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NCBI/Uniprot data below describe general gene information for B7-H4. It may not necessarily be applicable to this product.
 NCBI GI #    74759262
 NCBI GeneID    79679
 NCBI Accession #    Q7Z7D3.1 [Other Products]
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 UniProt Primary Accession #    Q7Z7D3 [Other Products]
 UniProt Secondary Accession #    Q0GN76; Q45VN0; Q5WPZ3; Q6P097; Q9H6B2 [Other Products]
 UniProt Related Accession #    Q7Z7D3 [Other Products]
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 NCBI Official Full Name    V-set domain-containing T-cell activation inhibitor 1
 NCBI Official Synonym Full Names    V-set domain containing T-cell activation inhibitor 1
 NCBI Official Symbol    VTCN1 [Similar Products]
 NCBI Official Synonym Symbols   
B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
[Similar Products]
 NCBI Protein Information    V-set domain-containing T-cell activation inhibitor 1
 UniProt Protein Name    V-set domain-containing T-cell activation inhibitor 1
 UniProt Synonym Protein Names   
B7 homolog 4; B7-H4; B7h.5; Immune costimulatory protein B7-H4; Protein B7S1; T-cell costimulatory molecule B7x
 UniProt Gene Name    VTCN1 [Similar Products]
 UniProt Synonym Gene Names    B7-H4 [Similar Products]
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 NCBI Summary for B7-H4    This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
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 UniProt Comments for B7-H4    VTCN1: Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor- associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.1-p12
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 Research Articles on B7-H4    1. study provided the first evidence that B7-H4 facilitated esophageal squamous cell carcinoma cell proliferation through promoting IL-6/STAT3 positive loopback pathway activation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-B7-H4 antibodyPathways associated with anti-B7-H4 antibody
 Reference Product  PubMed Publications
 FOXP3 antibody  >9 publications with B7-H4 and FOXP3
 CTLA4 antibody  >6 publications with B7-H4 and CTLA4
 BTLA antibody  >4 publications with B7-H4 and BTLA
 PDCD1LG2 antibody  >3 publications with B7-H4 and PDCD1LG2
 ICOSLG antibody  >2 publications with B7-H4 and ICOSLG
 TREML2 antibody  >1 publications with B7-H4 and TREML2
 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
Diseases associated with anti-B7-H4 antibodyOrgans/Tissues associated with anti-B7-H4 antibody
 Disease Name  Pubmed Publications
 Ovarian Neoplasms Antibodies  >20 publications with B7-H4 and Ovarian Neoplasms
 Disease Models, Animal Antibodies  >15 publications with B7-H4 and Disease Models, Animal
 Breast Neoplasms Antibodies  >14 publications with B7-H4 and Breast Neoplasms
 Inflammation Antibodies  >10 publications with B7-H4 and Inflammation
 Neoplasm Metastasis Antibodies  >10 publications with B7-H4 and Neoplasm Metastasis
 Lung Neoplasms Antibodies  >9 publications with B7-H4 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >9 publications with B7-H4 and Neoplasms, Experimental
 Kidney Diseases Antibodies  >6 publications with B7-H4 and Kidney Diseases
 Cell Transformation, Neoplastic Antibodies  >6 publications with B7-H4 and Cell Transformation, Neoplastic
 Necrosis Antibodies  >6 publications with B7-H4 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >11 publications with B7-H4 and Lung
 Pancreas Antibodies  >10 publications with B7-H4 and Pancreas
 Kidney Antibodies  >7 publications with B7-H4 and Kidney
 Liver Antibodies  >6 publications with B7-H4 and Liver
 Bone Antibodies  >5 publications with B7-H4 and Bone
 Uterus Antibodies  >4 publications with B7-H4 and Uterus
 Prostate Antibodies  >3 publications with B7-H4 and Prostate
 Ovary Antibodies  >3 publications with B7-H4 and Ovary
 Placenta Antibodies  >1 publications with B7-H4 and Placenta
 Bladder Antibodies  >1 publications with B7-H4 and Bladder
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