Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow BAX rightarrow Monoclonal BAX  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • CA 19-9 Native Protein
 • Claudin 5 (CLDN5) Antibody
 • 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B) ELISA Kit
 • CD8a (Ly 2) (CD8a (Ly 2)) Antibody
 • Cluster of Differentiation 226 (CD226) ELISA Kit
 • CD3-CD20 Dual Color Reagent
 • Goat anti Rabbit IgG (H + L) (HRP) Secondary Antibody
 • Apolipoprotein E (APOE) ELISA Kit
 • Acetylcholine receptor subunit alpha (ACHA) Recombinant Protein
 • ST6GALNAC1 Antibody
 • Complement 4d (C4d) (C4A) Antibody
 • Salmonella Antibody
 • Pancreatic Polypeptide (PP) ELISA Kit
 • Anti ganglioside antibody (GMI) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-BAX antibody :: Rabbit BAX Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS302765
Unit / Price
  DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

BAX, Monoclonal Antibody

 Also Known As   

Rabbit Anti-Human Bax Monoclonal Antibody (Clone SP47)

 Product Gene Name   

anti-BAX antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    600040
Section Bar
 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    SP47
 Host    Rabbit
 Species Reactivity    Human
Predicted Reactivity: Bovine, Mouse, Rat
Section Bar
 Form/Format    0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
Section Bar
 Immunogen    Synthetic peptide corresponding to internal region of human BAX protein.
 Control Tissue    Breast, Colon
 Cellular Localization    Cytoplasm, Membrane
 Entrez Gene Code    581
 Pathway    Apoptosis
Section Bar
 Preparation and Storage    Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
 Other Notes    Small volumes of anti-BAX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for anti-BAX antibody

   Bax is a member of the Bcl-2 family of proteins, which function as regulators of apoptosis.Overexpression of Bax functions to promote cell death. Bax can form homodimers and is also ableto heterodimerize with other Bcl-2 related proteins.
 Applications Tested/Suitable for anti-BAX antibody   

Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS)

 Application Notes for anti-BAX antibody    Immunohistochemistry Procedure
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilutions: If using the concentrate format of this product, dilute the antibody 1:50 prior to use. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at room temperature for 20 min. Primary Antibody: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Immunohistochemistry Dilution: 1:50
IHC Positive Control: Normal Breast

Flow Cytometry
Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 30 minutes at 4 degree C. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user.
Flow Cytometry Positive Control: HeLa Cell Line
Section Bar
 Immunohistochemistry (IHC) of anti-BAX antibody    Human normal breast stained with anti-BAX antibody
anti-BAX antibody Immunohistochemistry (IHC) (IHC) image
Section Bar
 Flow Cytometry (FC/FACS)|Flow cytometric analysis of rabbit anti-BAX (SP47) antibody in HeLa (green) compare to negative control of rabbit IgG (blue) of anti-BAX antibody    anti-BAX antibody Flow Cytometry (FC/FACS)|Flow cytometric analysis of rabbit anti-BAX (SP47) antibody in HeLa (green) compare to negative control of rabbit IgG (blue) (FC/FACS) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for BAX. It may not necessarily be applicable to this product.
 NCBI GI #    4757838
 NCBI GeneID    581
 NCBI Accession #    NP_004315.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004324.3 [Other Products]
Section Bar
 UniProt Secondary Accession #    P55269; Q07814; Q07815; Q8WZ49; Q9NR76; Q9NYG7; Q9UCZ6; Q9UCZ7; Q9UQD6; A8K4W1 [Other Products]
 UniProt Related Accession #    Q07812 [Other Products]
 Molecular Weight    21 kDa
Table BarTOPTable Bar
 NCBI Official Full Name    apoptosis regulator BAX isoform beta
 NCBI Official Synonym Full Names    BCL2-associated X protein
 NCBI Official Symbol    BAX [Similar Products]
 NCBI Official Synonym Symbols   
BCL2L4
[Similar Products]
 NCBI Protein Information    apoptosis regulator BAX; BCL2-associated X protein omega; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; bcl-2-like protein 4; bcl2-L-4
 UniProt Protein Name    Apoptosis regulator BAX
 UniProt Synonym Protein Names   
Bcl-2-like protein 4; Bcl2-L-4
 Protein Family    Bax inhibitor
 UniProt Gene Name    BAX [Similar Products]
 UniProt Synonym Gene Names    BCL2L4; Bcl2-L-4 [Similar Products]
 UniProt Entry Name    BAX_HUMAN
Section Bar
 NCBI Summary for BAX    The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for BAX    BAX: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Interacts with human cytomegalovirus/HHV-5 protein vMIA/UL37. Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. Belongs to the Bcl-2 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Tumor suppressor; Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial permeability transition pore complex; pore complex; endoplasmic reticulum membrane; mitochondrial outer membrane; membrane; mitochondrion; endoplasmic reticulum; nuclear envelope; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; channel activity; BH3 domain binding; lipid binding

Biological Process: hypothalamus development; viral reproduction; positive regulation of apoptosis; regulation of cell cycle; response to toxin; myeloid cell homeostasis; germ cell programmed cell death; B cell apoptosis; germ cell development; regulation of mammary gland epithelial cell proliferation; spermatid differentiation; regulation of mitochondrial membrane potential; development of secondary sexual characteristics; protein insertion into mitochondrial membrane during induction of apoptosis; establishment and/or maintenance of transmembrane electrochemical gradient; negative regulation of neuron apoptosis; negative regulation of protein binding; kidney development; release of cytochrome c from mitochondria; positive regulation of B cell apoptosis; regulation of protein homodimerization activity; vagina development; protein oligomerization; unfolded protein response, activation of signaling protein activity; fertilization; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; negative regulation of fibroblast proliferation; retina development in camera-type eye; reduction of endoplasmic reticulum calcium ion concentration; glycosphingolipid metabolic process; mitochondrial fragmentation during apoptosis; cerebral cortex development; regulation of nitrogen utilization; post-embryonic camera-type eye morphogenesis; regulation of protein heterodimerization activity; positive regulation of pigmentation; T cell homeostatic proliferation; apoptosis; neuron migration; positive regulation of apoptosis involved in mammary gland involution; negative regulation of peptidyl-serine phosphorylation; response to salt stress; release of matrix enzymes from mitochondria; positive regulation of protein oligomerization; apoptotic mitochondrial changes; B cell homeostatic proliferation; B cell homeostasis; ovarian follicle development; positive regulation of neuron apoptosis; response to gamma radiation; response to axon injury; B cell negative selection; protein homooligomerization; caspase activation; mitochondrial fusion; transformed cell apoptosis; Sertoli cell proliferation; odontogenesis of dentine-containing teeth; neuron apoptosis; endoplasmic reticulum calcium ion homeostasis; homeostasis of number of cells within a tissue; blood vessel remodeling; retinal cell programmed cell death; caspase activation via cytochrome c; positive regulation of release of sequestered calcium ion into cytosol
Table BarTOPTable Bar
 Research Articles on BAX    1. Binding to membranes is regulated by cleavage of Bid to truncated Bid (tBid), by conformation changes in tBid and Bax, and by interactions with other proteins.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with anti-BAX antibodyDiseases associated with anti-BAX antibody
 Products by Pathway  Pathway Diagram
 Activation, Translocation And Oligomerization Of BAX Pathway antibodies  Activation, Translocation And Oligomerization Of BAX Pathway Diagram
 AhR Pathway antibodies  AhR Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >2021 publications with BAX and Necrosis
 Nervous System Diseases Antibodies  >1868 publications with BAX and Nervous System Diseases
 Heart Diseases Antibodies  >1853 publications with BAX and Heart Diseases
 Ischemia Antibodies  >1549 publications with BAX and Ischemia
 Brain Diseases Antibodies  >1395 publications with BAX and Brain Diseases
 Inflammation Antibodies  >1032 publications with BAX and Inflammation
 Liver Diseases Antibodies  >970 publications with BAX and Liver Diseases
 Breast Neoplasms Antibodies  >963 publications with BAX and Breast Neoplasms
 Intestinal Neoplasms Antibodies  >839 publications with BAX and Intestinal Neoplasms
 Neoplasms, Experimental Antibodies  >770 publications with BAX and Neoplasms, Experimental
Organs/Tissues associated with anti-BAX antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3175 publications with BAX and Blood
 Brain Antibodies  >2457 publications with BAX and Brain
 Liver Antibodies  >1749 publications with BAX and Liver
 Lung Antibodies  >1338 publications with BAX and Lung
 Bone Antibodies  >791 publications with BAX and Bone
 Nerve Antibodies  >737 publications with BAX and Nerve
 Prostate Antibodies  >724 publications with BAX and Prostate
 Skin Antibodies  >518 publications with BAX and Skin
 Stomach Antibodies  >503 publications with BAX and Stomach
 Eye Antibodies  >403 publications with BAX and Eye
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions