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anti-BRPF1 antibody :: Mouse anti-Human BRPF1 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS120114
Unit / Price
0.1 mg  /  $345 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

BRPF1, Monoclonal Antibody

★Popular Item★
 Also Known As   

Mouse monoclonal antibody Anti-Human BRPF1

 Product Synonym Names    Homo sapiens bromodomain and PHD finger containing, 1 (BRPF1), transcript variant 1, mRNA; BR140
 Product Gene Name   

anti-BRPF1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    602410
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    2093C5a
 Host    Mouse
 Species Reactivity    Human
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 Concentration    100 ug/ml (1.0 ml) (lot specific)
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 Preparation    This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
 Sterility    Filtered through a 0.22 um membrane.
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 Other Notes    Small volumes of anti-BRPF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-BRPF1 antibody

   The protein encoded by this gene is expressed ubiquitously and at the highest level in testes and spermatogonia. The protein is localized within nuclei, and it is very similar in structure to two zinc finger proteins, AF10 and AF17. It is suggested that these proteins form a family of regulatory proteins. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [NCBI Entrez Gene Summary]
 Applications Tested/Suitable for anti-BRPF1 antibody   

Dot Blot (DB), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)

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 Quality Control of anti-BRPF1 antibody    Western blot analysis of immunized recombinant protein, using anti-BRPF1 monoclonal antibody.
anti-BRPF1 antibody Quality Control image
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 Quality Control #2 of anti-BRPF1 antibody    Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
anti-BRPF1 antibody Quality Control #2 image
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 Western Blot (WB) of anti-BRPF1 antibody    Detection of BRPF1 by Western blot.

Samples: Whole cell lysate from human HeLa (H, 50 ug) and rat NIH3T3 (M, 50 ug) cells. [Lot No. 2093C5a-1]

Predicted molecular weight: 138 kDa
anti-BRPF1 antibody Western Blot (WB) (WB) image
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 Immunoprecipitation (IP) of anti-BRPF1 antibody    Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-BRPF1 mAb. [Lot No. 2093C5a-1]

Predicted molecular weight: 138 kDa
anti-BRPF1 antibody Immunoprecipitation (IP) (IP) image
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 Flow Cytometry (FC/FACS) of anti-BRPF1 antibody    HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-BRPF1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2093C5a-1]
anti-BRPF1 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
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NCBI/Uniprot data below describe general gene information for BRPF1. It may not necessarily be applicable to this product.
 NCBI GI #    51173719
 NCBI GeneID    7862
 NCBI Accession #    NP_001003694.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001003694 [Other Products]
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 UniProt Secondary Accession #    Q7Z6E0; Q8TCM6; Q9UHI0; B4DEZ6 [Other Products]
 UniProt Related Accession #    P55201 [Other Products]
 Molecular Weight    127,363 Da
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 NCBI Official Full Name    Homo sapiens bromodomain and PHD finger containing, 1 (BRPF1), transcript variant 1, mRNA
 NCBI Official Synonym Full Names    bromodomain and PHD finger containing, 1
 NCBI Official Symbol    BRPF1 [Similar Products]
 NCBI Official Synonym Symbols   
BR140
[Similar Products]
 NCBI Protein Information    peregrin; bromodomain and PHD finger-containing protein 1; bromodomain-containing protein, 140kD
 UniProt Protein Name    Peregrin
 UniProt Synonym Protein Names   
Bromodomain and PHD finger-containing protein 1; Protein Br140
 Protein Family    Peregrin
 UniProt Gene Name    BRPF1 [Similar Products]
 UniProt Entry Name    BRPF1_HUMAN
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 NCBI Summary for BRPF1    The protein encoded by this gene is expressed ubiquitously and at the highest level in testes and spermatogonia. The protein is localized within nuclei, and it is very similar in structure to two zinc finger proteins, AF10 and AF17. It is suggested that these proteins form a family of regulatory proteins. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for BRPF1    peregrin: Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Positively regulates the transcription of RUNX1 and RUNX2. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 3p26-p25

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent
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 Research Articles on BRPF1    1. critical insights into the molecular mechanism of ligand binding by the BRPF1 bromodomain
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-BRPF1 antibodyPathways associated with anti-BRPF1 antibody
 Reference Product  PubMed Publications
 ING5 antibody  >4 publications with BRPF1 and ING5
 BRD1 antibody  >3 publications with BRPF1 and BRD1
 BRPF3 antibody  >2 publications with BRPF1 and BRPF3
 MYST3 antibody  >1 publications with BRPF1 and MYST3
 Products by Pathway  Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 HATs Acetylate Histones Pathway antibodies  HATs Acetylate Histones Pathway Diagram
Diseases associated with anti-BRPF1 antibodyOrgans/Tissues associated with anti-BRPF1 antibody
 Disease Name  Pubmed Publications
 Fetal Death Antibodies  >1 publications with BRPF1 and Fetal Death
 Liver Neoplasms Antibodies  >1 publications with BRPF1 and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with BRPF1 and Brain
 Testis Antibodies  >3 publications with BRPF1 and Testis
 Bone Antibodies  >2 publications with BRPF1 and Bone
 Liver Antibodies  >2 publications with BRPF1 and Liver
 Blood Antibodies  >2 publications with BRPF1 and Blood
 Embryonic Tissue Antibodies  >2 publications with BRPF1 and Embryonic Tissue
 Prostate Antibodies  >1 publications with BRPF1 and Prostate
 Umbilical Cord Antibodies  >1 publications with BRPF1 and Umbilical Cord
 Spleen Antibodies  >1 publications with BRPF1 and Spleen
 Placenta Antibodies  >1 publications with BRPF1 and Placenta
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