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anti-BUB1B antibody :: Mouse anti-Human BUB1B Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS830973
Unit / Price
0.1 mL  /  $515 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

BUB1B, Monoclonal Antibody

 Also Known As   

BUB1B antibody

 Product Synonym Names    Monoclonal BUB1B; Anti-BUB1B; Budding uninhibited by benzimidazoles 1 homolog beta; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta; BUB1B; BUBB 1; BUBB-1
 Product Gene Name   

anti-BUB1B antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    176430
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    10 E7
 Host    Mouse
 Species Reactivity    Human
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 Purity/Purification    BUB1B antibody was purified by affinity chromatography.
 Form/Format    Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
 Concentration    500 ug-1 mg/ml (lot specific)
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 Biological Significance    BUB1B is a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
 Immunogen    BUB1B antibody was raised in mouse using a protein expressed in 293T cell transfected with human BUB1B expression vector as the immunogen.
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 Preparation and Storage    Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
 Other Notes    Small volumes of anti-BUB1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-BUB1B antibody

   Mouse monoclonal BUB1B antibody
 Product Categories/Family for anti-BUB1B antibody    DNA & RNA
 Applications Tested/Suitable for anti-BUB1B antibody   

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

 Application Notes for anti-BUB1B antibody    IF: 1:100
IHC: 1:50
WB: 1:1000
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 Western Blot (WB) of anti-BUB1B antibody    Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BUB1B protein (Right) or empty vector (Left) detected with BUB1B antibody
anti-BUB1B antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-BUB1B antibody    Immunohistochemical analysis of BUB1B protein in paraffin embedded Human endometrium tissue using BUB1B antibody
anti-BUB1B antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for BUB1B. It may not necessarily be applicable to this product.
 NCBI GI #    294992075
 NCBI GeneID    701
 NCBI Accession #    ADF57218.1 [Other Products]
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 UniProt Secondary Accession #    O60501; O60627; O60758; O75389; Q59HH6; Q8WV50; Q96KM4; B2R6U0; B4DL09; B4DLG3 [Other Products]
 UniProt Related Accession #    O60566 [Other Products]
 Molecular Weight    121,386 Da
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 NCBI Official Full Name    BUB1B, partial
 NCBI Official Synonym Full Names    BUB1 mitotic checkpoint serine/threonine kinase B
 NCBI Official Symbol    BUB1B [Similar Products]
 NCBI Official Synonym Symbols   
MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta
[Similar Products]
 NCBI Protein Information    mitotic checkpoint serine/threonine-protein kinase BUB1 beta
 UniProt Protein Name    Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
 UniProt Synonym Protein Names   
MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1
 Protein Family    Mitotic checkpoint serine/threonine-protein kinase
 UniProt Gene Name    BUB1B [Similar Products]
 UniProt Synonym Gene Names    BUBR1; MAD3L; SSK1; hBUBR1 [Similar Products]
 UniProt Entry Name    BUB1B_HUMAN
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 NCBI Summary for BUB1B    This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq, Jul 2008]
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 UniProt Comments for BUB1B    BUB1B: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. Interacts with CENPE, CENPF, mitosin, PLK1 and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase-promoting complex/cyclosome (APC/C). Interacts with CASC5. Induced during mitosis. Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. Kinase activity stimulated by CENPE. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; EC 2.7.11.1; Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; BUB family

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: kinetochore; anaphase-promoting complex; perinuclear region of cytoplasm; cytoplasm; microtubule organizing center; outer kinetochore of condensed chromosome; spindle midzone; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: mitosis; cell proliferation; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; apoptosis; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; protein amino acid phosphorylation; mitotic metaphase/anaphase transition

Disease: Mosaic Variegated Aneuploidy Syndrome 1; Premature Chromatid Separation Trait
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 Research Articles on BUB1B    1. Bub1, but not BubR1, enhances binding of Bub3 to phosphorylated kinetochores.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-BUB1B antibodyDiseases associated with anti-BUB1B antibody
 Products by Pathway  Pathway Diagram
 APC-Cdc20 Mediated Degradation Of Nek2A Pathway antibodies  APC-Cdc20 Mediated Degradation Of Nek2A Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway antibodies  APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Cdc20:Phospho-APC/C Mediated Degradation Of Cyclin A Pathway antibodies  Cdc20:Phospho-APC/C Mediated Degradation Of Cyclin A Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >16 publications with BUB1B and Carcinoma
 Breast Neoplasms Antibodies  >12 publications with BUB1B and Breast Neoplasms
 Adenocarcinoma Antibodies  >10 publications with BUB1B and Adenocarcinoma
 Colorectal Neoplasms Antibodies  >10 publications with BUB1B and Colorectal Neoplasms
 Mosaic variegated aneuploidy syndrome Antibodies  >7 publications with BUB1B and Mosaic variegated aneuploidy syndrome
 Cell Transformation, Neoplastic Antibodies  >7 publications with BUB1B and Cell Transformation, Neoplastic
 Nervous System Diseases Antibodies  >6 publications with BUB1B and Nervous System Diseases
 Microcephaly Antibodies  >6 publications with BUB1B and Microcephaly
 Disease Models, Animal Antibodies  >6 publications with BUB1B and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >5 publications with BUB1B and Neoplasms, Experimental
Organs/Tissues associated with anti-BUB1B antibody
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >9 publications with BUB1B and Muscle
 Blood Antibodies  >9 publications with BUB1B and Blood
 Brain Antibodies  >7 publications with BUB1B and Brain
 Bone Antibodies  >6 publications with BUB1B and Bone
 Lung Antibodies  >6 publications with BUB1B and Lung
 Connective Tissue Antibodies  >4 publications with BUB1B and Connective Tissue
 Intestine Antibodies  >4 publications with BUB1B and Intestine
 Bone Marrow Antibodies  >3 publications with BUB1B and Bone Marrow
 Liver Antibodies  >3 publications with BUB1B and Liver
 Skin Antibodies  >3 publications with BUB1B and Skin
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