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anti-Bcl2 antibody :: Rabbit anti-Human Bcl 2 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS609596 anti-Bcl2 antibody
Unit / Price
0.1 mL  /  $580 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Bcl 2 (Bcl2), Monoclonal Antibody

 Also Known As   

Bcl 2, phosphorylated (Ser70) (B Cell Leukemia 2)

 Product Synonym Names    Anti -Bcl 2, phosphorylated (Ser70) (B Cell Leukemia 2)
 Product Gene Name   

anti-Bcl2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 13; NC_005112.2 (12730736..12905108, complement). Location: 13p13
 3D Structure    ModBase 3D Structure for P49950
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 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    7A4
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    Detects endogenous levels of human Ser70 phosphorylated Bcl 2. It does not crossreact with non-phosphorylated Bcl 2 at endogenous levels or with other Bcl 2 family members.
 Purity/Purification    Affinity Purified
Purified by Protein A affinity chromatography.
 Form/Format    Supplied as liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
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 Immunogen    Synthetic phosphopeptide corresponding to residues surrounding Ser70 of human Bcl 2 (KLH coupled).
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 Preparation and Storage    May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of anti-Bcl2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-Bcl2 antibody

   Bcl 2 has the ability to exert a survival function in response to a wide range of apoptotic stimuli by blocking mitochondrial release of cytochrome c (1). It has been implicated in modulating mitochondrial Ca2+ homeostasis and proton flux (2). Several phosphorylation sites have been identified within the Bcl 2 loop domain, including serine 70 (3). It has been suggested that phosphorylation at Ser70 may involve an ASK1/MKK7/JNK1 pathway and may be a marker for mitotic events (4,5). Interleukin 3 and JNK induced Bcl 2 phosphorylation at Ser70 may be required for its enhanced antiapoptotic functions (6).
 Product Categories/Family for anti-Bcl2 antibody    Antibodies; Abs to Apoptosis
 Applications Tested/Suitable for anti-Bcl2 antibody   

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

 Application Notes for anti-Bcl2 antibody    Suitable for use in ELISA, Flow Cytometry and Western Blot.
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS,
0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Western blot analysis of extracts from Jurkat cells, untreated or treated with paclitaxel (1uM, overnight) and with or without phosphatase, using phospho-Bcl-2 (Ser70) Rabbit mAb (upper) or Bcl-2 (lower).
Flow Cytometry: 1:25. Flow cytometric analysis of Jurkat cells, using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb versus propidium iodide (DNA content).
Immunoprecipitation: 1:50
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NCBI/Uniprot data below describe general gene information for Bcl2. It may not necessarily be applicable to this product.
 NCBI GI #    1004379
 NCBI GeneID    24224
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 UniProt Primary Accession #    P49950 [Other Products]
 UniProt Secondary Accession #    Q62837; Q64032 [Other Products]
 UniProt Related Accession #    P49950; Q7TSN8; Q9R1B3 [Other Products]
 Molecular Weight    26,622 Da [Similar Products]
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 NCBI Official Full Name    BCL-2
 NCBI Official Synonym Full Names    B-cell CLL/lymphoma 2
 NCBI Official Symbol    Bcl2 [Similar Products]
 NCBI Official Synonym Symbols   
Bcl-2
[Similar Products]
 NCBI Protein Information    apoptosis regulator Bcl-2; Bcl2-like protein; B-cell leukemia/lymphoma 2; B cell lymphoma 2 associated oncogene
 UniProt Protein Name    Apoptosis regulator Bcl-2
 Protein Family    Apoptosis regulator
 UniProt Gene Name    Bcl2 [Similar Products]
 UniProt Synonym Gene Names    Bcl-2 [Similar Products]
 UniProt Entry Name    BCL2_RAT
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 NCBI Summary for Bcl2    an anti-apoptotic protein; involved in inhibiting cell death in many different cell types [RGD]
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 UniProt Comments for Bcl2    Bcl-2: a antiapoptotic member of the Bcl-2 family. Regulates cell death by controlling the mitochondrial membrane permeability. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Phosphorylation by JNKs may increase its antiapoptotic functions.

Protein type: Apoptosis; Membrane protein, integral; Autophagy; Oncoprotein

Cellular Component: endoplasmic reticulum membrane; nuclear membrane; mitochondrion; endoplasmic reticulum; cell; integral to membrane; cytosol; pore complex; mitochondrial outer membrane; membrane; cytoplasm; mitochondrial membrane; intracellular; nucleus; myelin sheath

Molecular Function: identical protein binding; BH domain binding; protein homodimerization activity; protease binding; protein phosphatase 2A binding; channel activity; channel inhibitor activity; protein phosphatase binding; transcription factor binding; protein binding; protein heterodimerization activity; sequence-specific DNA binding; ubiquitin protein ligase binding; BH3 domain binding

Biological Process: positive regulation of catalytic activity; focal adhesion formation; developmental growth; pigment granule organization and biogenesis; regulation of cell cycle; response to toxin; ear development; negative regulation of myeloid cell apoptosis; B cell receptor signaling pathway; regulation of protein localization; negative regulation of ossification; oligodendrocyte differentiation; regulation of transmembrane transporter activity; negative regulation of neuron apoptosis; kidney development; T cell differentiation; response to drug; spleen development; positive regulation of neuron maturation; axon regeneration; cell aging; regulation of calcium ion transport; positive regulation of cell growth; response to ethanol; DNA damage response, signal transduction resulting in induction of apoptosis; response to estrogen stimulus; regulation of gene expression; response to heat; negative regulation of osteoblast proliferation; response to oxidative stress; lymphocyte homeostasis; negative regulation of apoptosis; positive regulation of pigmentation; positive regulation of smooth muscle cell migration; response to L-ascorbic acid; response to insulin stimulus; response to organic substance; negative regulation of cell proliferation; positive regulation of skeletal muscle fiber development; ureteric bud development; positive regulation of cell proliferation; negative regulation of cell migration; transmembrane transport; regulation of viral genome replication; response to nutrient; leukocyte homeostasis; aging; mesenchymal cell development; response to aluminum ion; CD8-positive, alpha-beta T cell lineage commitment; response to inorganic substance; B cell lineage commitment; positive regulation of peptidyl-serine phosphorylation; response to UV-B; cell proliferation; endoplasmic reticulum calcium ion homeostasis; peptidyl-serine phosphorylation; response to copper ion; pigmentation; ureteric bud branching; homeostasis of number of cells within a tissue; response to hypoxia; negative regulation of cell growth; brain development; response to nicotine; renal system process; protein polyubiquitination; response to glucocorticoid stimulus; T cell differentiation in the thymus; lymphoid progenitor cell differentiation; positive regulation of multicellular organism growth; response to organic cyclic substance; glomerulus development; post-embryonic development; response to caffeine; cochlear nucleus development; regulation of apoptosis; cellular response to glucose starvation; cell-cell adhesion; regulation of mitochondrial membrane potential; response to folic acid; positive regulation of B cell proliferation; T cell homeostasis; negative regulation of mitotic cell cycle; cell growth; response to electrical stimulus; defense response to virus; response to corticosterone stimulus; release of cytochrome c from mitochondria; regulation of protein homodimerization activity; actin filament organization; digestive tract morphogenesis; organ growth; organ morphogenesis; induction of apoptosis via death domain receptors; response to steroid hormone stimulus; B cell differentiation; gland morphogenesis; regulation of pigmentation during development; regulation of mitochondrial membrane permeability; regulation of nitrogen utilization; metanephros development; oocyte development; B cell proliferation; response to peptide hormone stimulus; regulation of protein heterodimerization activity; response to cAMP; behavioral fear response; melanin metabolic process; negative regulation of retinal cell programmed cell death; apoptosis; regulation of cell-matrix adhesion; pigmentation during development; cell morphogenesis; regulation of protein stability; protein amino acid dephosphorylation; apoptotic mitochondrial changes; ovarian follicle development; B cell homeostasis; positive regulation of melanocyte differentiation; regulation of catalytic activity; melanocyte differentiation; response to gamma radiation; hemopoiesis; negative regulation of cellular pH reduction; response to iron ion; ossification; hair follicle morphogenesis; thymus development; male gonad development; peptidyl-threonine phosphorylation; regulation of programmed cell death; cellular calcium ion homeostasis; immune system development; T cell lineage commitment; neuron apoptosis; axonogenesis; response to hydrogen peroxide; response to cytokine stimulus; induction of apoptosis by oxidative stress; response to DNA damage stimulus; growth
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 Research Articles on Bcl2    1. These results indicated that the Bcl-2 and Bax played a pivotal role in the gastric mucosal I-R injury and repair by activation of ERK1/2.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-Bcl2 antibodyPathways associated with anti-Bcl2 antibody
 Reference Product  PubMed Publications
 Bax antibody  >731 publications with Bcl2 and Bax
 Bcl2l11 antibody  >5 publications with Bcl2 and Bcl2l11
 Products by Pathway  Pathway Diagram
 ATM Pathway antibodies  ATM Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
Diseases associated with anti-Bcl2 antibodyOrgans/Tissues associated with anti-Bcl2 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1723 publications with Bcl2 and Neoplasms
 Lymphoma Antibodies  >752 publications with Bcl2 and Lymphoma
 Lymphoma, B-Cell Antibodies  >532 publications with Bcl2 and Lymphoma, B-Cell
 Leukemia Antibodies  >437 publications with Bcl2 and Leukemia
 Carcinoma Antibodies  >403 publications with Bcl2 and Carcinoma
 Lymphoma, Follicular Antibodies  >278 publications with Bcl2 and Lymphoma, Follicular
 Breast Neoplasms Antibodies  >213 publications with Bcl2 and Breast Neoplasms
 Adenocarcinoma Antibodies  >155 publications with Bcl2 and Adenocarcinoma
 Leukemia, Myeloid Antibodies  >154 publications with Bcl2 and Leukemia, Myeloid
 Neoplasms, Experimental Antibodies  >132 publications with Bcl2 and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >180 publications with Bcl2 and Brain
 Spleen Antibodies  >44 publications with Bcl2 and Spleen
 Artery Antibodies  >41 publications with Bcl2 and Artery
 Pituitary Gland Antibodies  >1 publications with Bcl2 and Pituitary Gland
 Uncharacterized Tissue Antibodies  >1 publications with Bcl2 and Uncharacterized Tissue
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