Specificity
Human Bcl-xL (Mr 29 kDa)
Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems.1 It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment.2 Bcl- 2 and one of its homologues, Bcl-xL, protect cells from apoptosis3,4, while other homologues of Bcl-2 such as Bax, Bad and Bak have been shown to enhance apoptosis.5-8 Bcl-xL has been shown to block apoptosis which is induced by a variety of stimuli and, under certain conditions, offers greater protection against apoptosis than Bcl-2.9-13 In contrast, Bad and Bax inhibit the protective functions of Bcl-xL and Bcl-2, respectively. Although heterodimerization between Bcl-xL/Bad and Bcl-2/Bax was originally thought to be essential for the differential anti-apoptotic activity of Bcl-xL and Bcl-2,5,14 other results suggest that the formation of heterodimers may not be necessary for this death-repressing activity.15,16 Mab 7B2.5 recognizes both human and rodent Bcl-xL.17
Product References and Citations for
anti-Bcl-xL antibody
1. Cohen, J.J. 1991. Adv. Immunol. 50:55.
2. Cohen, J.J., and R.C. Duke. 1992. Annu. Rev. Immunol. 10:2676.
3. Nunez, G., and M.F. Clarke. 1994. Trends Cell Biol. 4:399.
4. Cory, S. 1995. Annu. Rev. Immunol. 13:513.
5. Oltvai., Z.N., C.L. Millman, and S.J. Korsmeyer. 1993. Cell 74:609.
6. Farrow, S.N., et al. 1995. Nature 374:731.
7. Chittenden, T., et al. 1995. Nature 374:733.
8. Kiefer, M.C., et al. 1995. Nature 374:736.
9. Boise, L.H., M. Gonzalez-Garcia, C.E. Postema, L. Ding, T. Lindstein, L.A. Turka, X. Mao, G. Nunez, and C.B. Thompson 1993. Cell 74:597.
10. Gottschalk, A.R., L.H. Boise, C.B. Thompson, and J. Quintans. 1994. Proc. Natl. Acad. Sci. USA 91:7350.
11. Gonzalez-Garcia, M., I. Garcia, L. Ding, S. O'Shea, L.H. Boise, C.B. Thompson, and G. Nunez. 1995. Proc. Natl. Acad. Sci. USA 92:4304
12. Dole, M.G., R. Jasty, M.J. Cooper, C.B. Thompson, G. Nunez, and V.P. Castle. 1995. Can. Res. 55:2576.
13. Shimizu, S., et al. 1995. Nature 374:811.
14. Yin, X.M., Z.N. Oltvai, and S.J. Korsmeyer. 1994. Nature 369:321.
15. Cheng, E.H.-Y., et al. 1996. Nature 379:554.
16. Gottschalk, A.R., L.H. Boise, Z.N. Oltvai, M.A. Accavitti, S.J. Korsmeyer, J. Quintans, and C.B. Thompson. 1996. Cell Death and Differentiation 3:113.
17. Boise, L.H. 1997. Personal communication.
18. L. Haughan. 1997. Personal communication.