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anti-HSPA5 antibody :: Rat anti-Mouse BiP Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS673495 anti-HSPA5 antibody
Unit / Price
0.5 mg  /  $325 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

BiP (HSPA5), Monoclonal Antibody

 Also Known As   

Rat Anti-Mouse BiP-UNLB

 Product Synonym Names    Rat Anti-Mouse BiP - Purified, Unlabeled Antibody; Rat Anti-Mouse BiP
 Product Gene Name   

anti-HSPA5 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 9; NC_000009.11 (127997127..128003661, complement). Location: 9q33.3
 OMIM    138120
 3D Structure    ModBase 3D Structure for P11021
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 Clonality    Monoclonal
 Isotype    Rat IgG1 kappa
 Clone Number    76-E6
 Host    Rat
 Species Reactivity    Mouse
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 Specificity    BiP (Immunoglobulin heavy chain binding protein, Mr 78 kDa)
The immunoglobulin heavy chain binding protein BiP (Binding Protein, [1]) is a member of the hsp70 family of heat shock proteins, and is identical to the glucose regulated protein grp78.2 While BiP was originally described for its function in B cells, it is now known to be distributed in a variety of tissues, if not ubiquitous. The highly conserved hsp 70 proteins have an essential physiological role in stress responses and as "molecular chaperones," which are responsible for a variety of functions such as protein transport, prevention of protein toxicity and direction of protein folding.1-5 With regard to its immunological role, BiP is a component of the endoplasmic reticulum and binds free intracellular heavy chains in nonsecreting pre-B cell lines (u+,L-) or incompletely assembled Ig precursors in H+L+ secreting hybridomas and myelomas. In the absence of light chain synthesis, heavy chains remain associated with BiP and are not secreted. BiP is an ATP binding protein and the dissociation of the BiP-heavy chain complex is probably driven by the ATPase activity attributed to BiP.7 Monoclonal antibody 76-E6 recognizes a conserved epitope localized within the region of amino acids 497 to 581 of BiP.8
 Form/Format    Purified (UNLB) Antibody
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 Characterization    To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform with characteristics of a standard reference reagent using Western blotting. Representative data are included in this data sheet.
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 Preparation and Storage    The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
 Other Notes    Small volumes of anti-HSPA5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-HSPA5 antibody   

ELISA; Western Blot

 Application Notes for anti-HSPA5 antibody    Research Application(s): Studies of immunoglobulin assembly and secretion
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NCBI/Uniprot data below describe general gene information for HSPA5. It may not necessarily be applicable to this product.
 NCBI GI #    1143492
 NCBI GeneID    3309
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 UniProt Primary Accession #    P11021 [Other Products]
 UniProt Secondary Accession #    Q2EF78; Q9NPF1; Q9UK02; B0QZ61 [Other Products]
 UniProt Related Accession #    P11021 [Other Products]
 Molecular Weight    72,333 Da [Similar Products]
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 NCBI Official Full Name    BiP
 NCBI Official Synonym Full Names    heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
 NCBI Official Symbol    HSPA5 [Similar Products]
 NCBI Official Synonym Symbols   
BIP; MIF2; GRP78; FLJ26106
[Similar Products]
 NCBI Protein Information    78 kDa glucose-regulated protein; OTTHUMP00000022124; immunoglobulin heavy chain-binding protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
 UniProt Protein Name    78 kDa glucose-regulated protein
 UniProt Synonym Protein Names   
Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein
 Protein Family    Biphenyl dioxygenase
 UniProt Gene Name    HSPA5 [Similar Products]
 UniProt Synonym Gene Names    GRP78 [Similar Products]
 UniProt Entry Name    GRP78_HUMAN
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 NCBI Summary for HSPA5    The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.
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 UniProt Comments for HSPA5    Function: Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Ref.12

Subunit structure: Interacts with DNAJC1 (via J domain)

By similarity. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with TMEM132A and TRIM21. May form a complex with ERLEC1, OS9, SEL1L and SYVN1. Ref.16 Ref.20 Ref.21

Subcellular location: Endoplasmic reticulum lumen. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.17 Ref.18

Involvement in disease: Note=Autoantigen in rheumatoid arthritis. Ref.10

Sequence similarities: Belongs to the heat shock protein 70 family.
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Product References and Citations for anti-HSPA5 antibody

   1. Bole, D.G., L.M. Hendershot, and J.F. Kearney. 1986. J. Cell Biol. 102:1558.
2. Haas, I.G. 1991. Current Topics Micro. & Immunol. 167:71.
3. Hendrick, J.P. and Hartl, F.U. 1991. FASEB J. 9(15):1559.
4. Pierce, S.K. 1994. Experientia 50(11-12):1026
5. Haas, I.G. 1994. Experientia 50(11-12):1012.
6. Wu, Q. 1996.
7. Kassenbrock, C.K. and Kelly, R.B. 1989. EMBO J. 8: 233.
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 Research Articles on HSPA5    1. Knockdown of GRP78 expression by siRNA did not effect viral RNA replication, but did impair mature viral production.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-HSPA5 antibodyPathways associated with anti-HSPA5 antibody
 Reference Product  PubMed Publications
 SIL1 antibody  >3 publications with HSPA5 and SIL1
 ERP29 antibody  >1 publications with HSPA5 and ERP29
 ERN1 antibody  >1 publications with HSPA5 and ERN1
 EIF2AK3 antibody  >1 publications with HSPA5 and EIF2AK3
 Products by Pathway  Pathway Diagram
 Activation Of Chaperones By ATF6-alpha Pathway antibodies  Activation Of Chaperones By ATF6-alpha Pathway Diagram
 Activation Of Chaperones By IRE1alpha Pathway antibodies  Activation Of Chaperones By IRE1alpha Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway antibodies  Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Diabetes Pathways antibodies  Diabetes Pathways Diagram
 Formation Of Platelet Plug Pathway antibodies  Formation Of Platelet Plug Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
Diseases associated with anti-HSPA5 antibodyOrgans/Tissues associated with anti-HSPA5 antibody
 Disease Name  Pubmed Publications
 Kidney disorder involving deposition of calcium and oxalate or phosphate in the renal tubules Antibodies  >2426 publications with HSPA5 and Kidney disorder involving deposition of calcium and oxalate or phosphate in the renal tubules
 Death Antibodies  >51 publications with HSPA5 and Death
 Drug Toxicity Antibodies  >24 publications with HSPA5 and Drug Toxicity
 Metabolic Diseases Antibodies  >18 publications with HSPA5 and Metabolic Diseases
 Neoplasms Antibodies  >16 publications with HSPA5 and Neoplasms
 Neurodegenerative Diseases Antibodies  >15 publications with HSPA5 and Neurodegenerative Diseases
 Brain Ischemia Antibodies  >13 publications with HSPA5 and Brain Ischemia
 Urologic Diseases Antibodies  >12 publications with HSPA5 and Urologic Diseases
 Inflammation Antibodies  >10 publications with HSPA5 and Inflammation
 Necrosis Antibodies  >9 publications with HSPA5 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >45 publications with HSPA5 and Brain
 Muscle Antibodies  >26 publications with HSPA5 and Muscle
 Kidney Antibodies  >20 publications with HSPA5 and Kidney
 Heart Antibodies  >18 publications with HSPA5 and Heart
 Liver Antibodies  >15 publications with HSPA5 and Liver
 Blood Antibodies  >15 publications with HSPA5 and Blood
 Testis Antibodies  >10 publications with HSPA5 and Testis
 Nerve Antibodies  >10 publications with HSPA5 and Nerve
 Vascular Antibodies  >10 publications with HSPA5 and Vascular
 Pancreas Antibodies  >9 publications with HSPA5 and Pancreas
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