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Mouse anti-All species Biotin Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS438174
Testing Data
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Biotin, Monoclonal Antibody

★Popular Item★
 Also Known As   

Biotin (Vitamin B7 or Vitamin H)

 Product Synonym Names    Vitamin B7 or Vitamin H
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    SPM375
 Host    Mouse
 Species Reactivity    All species
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 Specificity    It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
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 Localization    Depends on the target
 Positive Control    Biotinylated proteins in solution or on tissues
 Immunogen    Biotinylated sheep immunoglobulin
 Quantity and Buffer    0.5 ml at 200ug/ml with BSA and azide
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 Preparation and Storage    Store at 2 to 8 degree C. Antibody is stable for 24 months.
 Other Notes    Small volumes of anti-Biotin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-Biotin antibody    CB- Cell Biology
 Applications Tested/Suitable for anti-Biotin antibody   

Confocal Microscopy, ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), In situ Nucleic Acid Hybridization, Western Blot (WB), Immunohistology (IH)

 Application Notes for anti-Biotin antibody    Confocal Microscopy; ELISA; Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); In situ Nucleic Acid Hybridization; Western Blotting; Immunohistology (Frozen & Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.) Optimal dilution for a specific application should be determined.
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 Testing Data of anti-Biotin antibody    Formalin-paraffin tonsil stained with biotinylated Lambda Light Chain probe followed by Biotin MAb (SPM375). Note cell membrane & cytoplasmic staining.
anti-Biotin antibody Testing Data image
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 Molecular Weight    244 Dalton [Similar Products]
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 Protein Family    Biotinidase

Product References and Citations for anti-Biotin antibody

   1. Uccellini MB, et al. 2008. J. Immunol. 181:5875.
2. Green NM, et al. 2012. J Biol Chem. 287:39789
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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