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anti-C4 antibody :: Rat anti-C4b
C4d C4 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS584166 anti-C4 antibody
Unit / Price
0.1 mg  /  $600 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

C4, Monoclonal Antibody

★Popular Item★
 Also Known As   

C4, Mouse, mAb 16D2

 Product Synonym Names    Complement component 4
 Product Gene Name   

anti-C4 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for P01029
 Clonality    Monoclonal
 Isotype    Rat IgG2a
 Host    Rat
 Species Reactivity    C4b
C4d
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 Form/Format    1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
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 Immunogen    Thymocytes decorated with Thy-1 antibody and complement components
 Positive Control    T-cell regions of mouse spleen
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 Preparation and Storage    Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
 Other Notes    Small volumes of anti-C4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-C4 antibody

   Product Description: The monoclonal antibody 16D2 recognizes mouse complement factor C4, formerly known as Gg protein, which consists of an alpha-, beta-, and gamma-chain. The classical pathway of complement and the Mannose binding lectin activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. Subsequently, C4b can be cleaved to C4c and C4d by other serum enzymes. The monoclonal antibody 16D2 reacts with intact C4, C4b and C4d.
C4 is an acute phase protein that is produced by hepatocytes, monocytes and intestinal epithelial cells and can be used- in experimental animals as a marker for activation of the classical complement pathway. Recent studies have demonstrated an association between graft rejection and C4d deposition in a mouse model for cardiac transplantation.
 Applications Tested/Suitable for anti-C4 antibody   

Immunoassay (IA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)

 Application Notes for anti-C4 antibody    Application Notes: W: A non-reduced sample treatment and SDS-Page was used. The band size is 200x kDa (Ref.4).
IA: Plates were coated overnight with rat anti-murine C4 mAb 16D2 in carbonate buffer and blocked with 5% dry milk in PBS and 0.01% Tween 20.
IF: Spleens were snap frozen and stored at -80 degree C until cryosections were cute. (Section (5um) were fixed for 4 mins in ice-cold acetone. Sections were blocked with 2% BSA, 2% FCS and PBS. Biotinylated 16D2 was visualized using avidin-PE.
Application Use: For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
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NCBI/Uniprot data below describe general gene information for C4. It may not necessarily be applicable to this product.
 NCBI GI #    287732
 NCBI GeneID    12268
 NCBI Accession #    CAA39114.1 [Other Products]
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 UniProt Primary Accession #    P01029 [Other Products]
 UniProt Secondary Accession #    O70346; Q31201; Q3TYY1; Q3TZC9; Q61372; Q61859; Q62353; Q6NWV8; E9QKK7 [Other Products]
 UniProt Related Accession #    P01029 [Other Products]
 Molecular Weight    192,915 Da
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 NCBI Official Full Name    C4, partial
 NCBI Official Synonym Full Names    complement component 4B (Chido blood group)
 NCBI Official Symbol    C4b [Similar Products]
 NCBI Official Synonym Symbols   
C4; Ss
[Similar Products]
 NCBI Protein Information    complement C4-B; complement component 4 (within H-2S); complement component 4B (Childo blood group)
 UniProt Protein Name    Complement C4-B
 Protein Family    Complement C4
 UniProt Gene Name    C4b [Similar Products]
 UniProt Synonym Gene Names    C4 [Similar Products]
 UniProt Entry Name    CO4B_MOUSE
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 UniProt Comments for C4    C4B: C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway. Defects in C4B are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Interindividual copy- number variation (CNV) of complement component C4 and associated polymorphisms result in different susceptibilities to SLE. The risk of SLE susceptibility has been shown to be significantly increased among subjects with only two copies of total C4. A high copy number is a protective factor against SLE. Defects in C4B are the cause of complement component 4B deficiency (C4BD). A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: complement binding; complement component C1q binding; carbohydrate binding

Biological Process: immunoglobulin mediated immune response; complement activation
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Product References and Citations for anti-C4 antibody

   1. Kremmer, E et al; Monoclonal antibodies to complement components without the need of their prior purification. II. Antibodies to mouse C3 and C4. Hybridoma 1990, 9 : 309 2. Gadjeva, M et al; Macrophage-derived complement component C4 can restore humoral immunity in C4-deficient mice. J Immunol 2002, 169: 5489
3. Paul E et al; Anti-DNA autoreactivity in C4-deficient mice. Eur J Immunol 2002, 32: 2672
4. Murata, K et al; Synergistic deposition of C4d by complement-activating and non-activating antibodies in cardiac transplants. Am J Transplant 2007, 7: 2605
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 Research Articles on C4    1. Data indicate complement C3 deposition on peanut extract (PE) was abolished in immunoglobulin- and C4-deficient sera.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-C4 antibodyDiseases associated with anti-C4 antibody
 Products by Pathway  Pathway Diagram
 Activation Of C3 And C5 Pathway antibodies  Activation Of C3 And C5 Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Complement Activation, Classical Pathway antibodies  Complement Activation, Classical Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Initial Triggering Of Complement Pathway antibodies  Initial Triggering Of Complement Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Disease Name  Pubmed Publications
 Autoimmune Diseases Antibodies  >352 publications with C4 and Autoimmune Diseases
 Kidney Diseases Antibodies  >178 publications with C4 and Kidney Diseases
 Inflammation Antibodies  >176 publications with C4 and Inflammation
 Necrosis Antibodies  >87 publications with C4 and Necrosis
 Liver Diseases Antibodies  >66 publications with C4 and Liver Diseases
 Hyperplasia Antibodies  >62 publications with C4 and Hyperplasia
 Brain Diseases Antibodies  >56 publications with C4 and Brain Diseases
 Death Antibodies  >55 publications with C4 and Death
 Heart Diseases Antibodies  >54 publications with C4 and Heart Diseases
 Fibrosis Antibodies  >45 publications with C4 and Fibrosis
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