View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List anti-Mouse CD103 (Integrin alpha IEL) Monoclonal Antibody Catalog # MBS673586 Unit / Price 0.5 mg / $445 +1 FREE 8GB USB 5x0.5 mg / $1,820 +2 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name CD103 (Integrin alpha IEL), Monoclonal Antibody Full Product Name Hamster Anti-Mouse CD103-FITC Product Synonym Names Hamster Anti-Mouse CD103 - FITC; Hamster Anti-Mouse CD103 (Integrin alpha IEL) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype Armenian Hamster IgG Clone Number 2E7 Host Armenian Hamster Species Reactivity Mouse Specificity The CD103/ alpha IEL beta 7 integrin complex (Mr 160 and 115 kDa peptides).CD103 is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery.1-3 In non-epithelial CD103+ T cells there is a bias toward expression on CD8+.cells. The 2E7 mAb exhibits antigen immunoprecipitation patterns similar if not identical to the rat anti-mouse CD103 mAb M290.1 The 2E7 mAb is reported to have signal-inducing activity in a redirected lysis assay and to co-stimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro, which runs counter to the usual inhibition of CTL by anti-integrins.1 This co-stimulatory activity is also shared by the mAb M290.4 The property of costimulation is not unique to the mouse since the combination of anti-human alpha IEL plus anti-TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated.5 Form/Format Fluorescein (FITC) Conjugate Characterization To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert. Preparation and Storage The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed. Other Notes Small volumes of anti-CD103 (Integrin alpha IEL) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Applications Tested/Suitable for anti-CD103 (Integrin alpha IEL) antibody Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation Application Notes for anti-CD103 (Integrin alpha IEL) antibody Research Application(s): Identification and enumeration of CD103 ^+ cells by flow cytometryImmunohistochemistry (frozen sections)In vitro activationFlow Cytometry Suggested Dilution: <= 1 ug/10^6 cells TOP Product References and Citations for anti-CD103 (Integrin alpha IEL) antibody 1. LeFrancois, L., T.A. Bennett, W.L. Havran, and L. Puddington. 1994. Developmental expression of the alpha IEL beta 7 integrin on T cell receptor gamma delta and T cell receptor alpha beta T cells. Eur. J. Immunol. 24:635.2. Lefrancois, L. 1991. Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal repertoire? Immunol. Today, 12(12):436.3. Reimann, J. and Rudolphi, A. 1995. Co-expression of CD8 alpha in CD4+ T cell receptor alpha beta + T cell migration into the murine small intestine epithelial layer. Eur. J. Immunol. 25:1580.4. Roberts, K., and P. Kilshaw. 1993. The mucosal T cell integrin alpha M290 beta 7 recognizes a ligand on mucosal epithelial cell lines. Eur. J. Immunol. 23:1630.5. Cerf-Bensussan, N., Bengue, B., Gagnon, J. and Meo, T. 1992. Eur. J. Immunol. 22:273. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP