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anti-CD105 antibody :: Mouse CD105 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS212208
Unit / Price
0.2 mg  /  $455 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD105, Monoclonal Antibody

 Also Known As   

MOUSE ANTI HUMAN CD105

 Product Gene Name   

anti-CD105 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    131195
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    SN6
 Host    Mouse
 Species Reactivity    Cynomolgus monkey, Horse, Monkey, Primate, Rhesus Monkey
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 Form/Format    Purified
Purified IgG - liquid
 Concentration    IgG concentration 1.0 mg/ml (lot specific)
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 Perservative Stabilisers    0.09% Sodium Azide
Preparation
 Immunogen    Partially purified cell membrane antigens from fresh leukaemia cells.
Fusion Partners
 Target Species    Human
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-CD105 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-CD105 antibody   

Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)

 Application Notes for anti-CD105 antibody    Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Maximum Dilution: 1/50
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 Testing Data of anti-CD105 antibody    Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 488 (MBS212208A488)
anti-CD105 antibody Testing Data image
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 Testing Data of anti-CD105 antibody    Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Expression of endothelial antigens in endothelium-adherent monocytes in co-culture with HCAECs.HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HCAECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by dual-colour flow cytometry for HLA-A2 and (A) CD105, (B) eNOS and (C) VEGFR2 expression on Day 2 of co-culture.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.
anti-CD105 antibody Testing Data image
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 Testing Data of anti-CD105 antibody    Staining of KG1 cells with Mouse anti Human CD105
anti-CD105 antibody Testing Data image
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 Testing Data of anti-CD105 antibody    Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 647 (MBS212208A647)
anti-CD105 antibody Testing Data image
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 Testing Data of anti-CD105 antibody    Staining of KG1 cells with Mouse anti Human CD105RPE (MBS213632)
anti-CD105 antibody Testing Data image
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 Testing Data of anti-CD105 antibody    Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Increased expression of CD105 and CD144 in the endothelium-adherent monocytes during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were maintained in co-culture up to Day 6, then assessed by dual-colour flow cytometry for HLA-A2 and (A) CD105 and (B) CD144 expression on Day 3 of co-culture. Representative plots from 4 -7 individual experiments are shown. The increase in CD105 from Day 0 to Day 6 (C) and CD144 expression from Day 0 to Day 6 (D) is also shown.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.
anti-CD105 antibody Testing Data image
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 Testing Data of anti-CD105 antibody    Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry on brain microvascular endothelial cellsImage caption:Characterization and functional features of human and murine BMVECs. (A) Phase contrast micrographs of confluent monolayers of human (left image) and murine (right image) BMVECs. BMVECs present the typical œcobblestone appearance. Scale bar, 100 um and 200 um for human BMVECs and murine BMVECs. B) Human (left image) and murine (right image) BMVECs showed a clear cytoplasmic staining for CD31. Scale bar, 50 um. C) Human (left image) and murine (right image) cells displayed an intense positive immunofluorescence for vWf. Scale bar, 50 um. D) Flow cytometric analysis of BMVECs. Human BMVECs resulted positive (gray histograms) for CD31 (left graph), CD105, CD146 (left gaph), UEA-1 staining; murine BMVECs resulted positive for CD31 (right graph), CD34, CD146 (right graph) and Tie-2 staining. White histograms represent the isotype controls of each antibody. E) Capillary tube-like structure produced by human (left image) and murine (right image) BMVECs, 7 h after plating onto Matrigel. Scale bar, 100 um. F) LDL-uptake assay on human (left image) and murine (right image) BMVECs. Scale bar, 50 um. G) Human (left image) and murine (right image) BMVECs were labelled for GLUT-1. Scale bar, 50 um. H) Immunofluorescence for eNOS in human (left image) and murine (right image) BMVECs. Scale bar, 50 um. All nuclei were counterstained with DAPI (blue). One representative of three independent experiments performed in blind is shown for each figure.From: Navone SE, Marfia G, Nava S, Invernici G, Cristini S, Balbi S, Sangiorgi S, Ciusani E, Bosutti A, Alessandri G, Slevin M, Parati EA. Human and mouse brain-derived endothelial cells require high levels of growth factors medium for their isolation, in vitro maintenance and survival. Vasc Cell. 2013 May 14;5(1):10.
anti-CD105 antibody Testing Data image
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 Testing Data of anti-CD105 antibody    Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Endothelial adherence of blood monocytes. PBMCs were isolated from HLA-A2+ donors and incubated with HLA-A2- HUVECs (1x106 cells/well) for 2 h, after which the non-adherent PBMCs were removed by washing. The co-cultured cell layers were immediately analysed with dual-colour flow cytometry for HLA-A2 and (A) CD34, (B) CD14, (C) CD11b, (D) CD16, (E) CD105 and (F) CD144 expression. Representative plots from 4 -6 individual experiments are shown. (G) Two parameters dot plot showing typical isotype controls.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.
anti-CD105 antibody Testing Data image
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 Testing Data of anti-CD105 antibody    Staining of KG1 cells with Mouse anti Human CD105:FITC (MBS210726)
anti-CD105 antibody Testing Data image
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 Testing Data of anti-CD105 antibody    Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.
anti-CD105 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for CD105. It may not necessarily be applicable to this product.
 NCBI GI #    4557555
 NCBI GeneID    2022
 NCBI Accession #    NP_000109.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000118.3 [Other Products]
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 UniProt Secondary Accession #    Q14248; Q14926; Q5T9C0 [Other Products]
 UniProt Related Accession #    P17813 [Other Products]
 Molecular Weight    67,542 Da
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 NCBI Official Full Name    endoglin isoform 2
 NCBI Official Synonym Full Names    endoglin
 NCBI Official Symbol    ENG [Similar Products]
 NCBI Official Synonym Symbols   
END; HHT1; ORW1
[Similar Products]
 NCBI Protein Information    endoglin; CD105 antigen
 UniProt Protein Name    Endoglin
 UniProt Synonym Protein Names   
CD_antigen: CD105
 UniProt Gene Name    ENG [Similar Products]
 UniProt Synonym Gene Names    END [Similar Products]
 UniProt Entry Name    EGLN_HUMAN
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 NCBI Summary for CD105    This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]
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 UniProt Comments for CD105    ENG: Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Homodimer that forms an heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Interacts with TCTEX1D4. Interacts with ARRB2. Endoglin is restricted to endothelial cells in all tissues except bone marrow. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: nucleoplasm; extracellular space; focal adhesion; cell surface; cytoplasm; receptor complex; external side of plasma membrane

Molecular Function: transforming growth factor beta receptor activity; protein binding; transmembrane receptor activity; protein homodimerization activity; glycosaminoglycan binding; transforming growth factor beta binding; galactose binding; punt binding; activin binding; transforming growth factor beta receptor, cytoplasmic mediator activity

Biological Process: wound healing; positive regulation of collagen biosynthetic process; cell motility involved in cell locomotion; positive regulation of systemic arterial blood pressure; negative regulation of transcription from RNA polymerase II promoter; response to corticosteroid stimulus; BMP signaling pathway; smooth muscle development; extracellular matrix disassembly; regulation of transforming growth factor beta receptor signaling pathway; venous blood vessel morphogenesis; central nervous system vasculogenesis; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; negative regulation of protein amino acid autophosphorylation; heart looping; vasculogenesis; cell adhesion; chronological cell aging; negative regulation of cell migration; positive regulation of BMP signaling pathway; regulation of cell adhesion; cell migration; negative regulation of nitric-oxide synthase activity; regulation of phosphorylation; regulation of cell proliferation; patterning of blood vessels; negative regulation of endothelial cell proliferation; response to hypoxia; artery morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway

Disease: Telangiectasia, Hereditary Hemorrhagic, Of Rendu, Osler, And Weber
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 Research Articles on CD105    1. Collectively, these findings establish Src and pro-angiogenic mitogens as critical mediators of endoglin stability and function
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CD105 antibodyPathways associated with anti-CD105 antibody
 Reference Product  PubMed Publications
 TGFB1 antibody  >329 publications with CD105 and TGFB1
 ACVRL1 antibody  >167 publications with CD105 and ACVRL1
 BMP2 antibody  >131 publications with CD105 and BMP2
 SMAD4 antibody  >51 publications with CD105 and SMAD4
 HIF1A antibody  >38 publications with CD105 and HIF1A
 TGFBR2 antibody  >6 publications with CD105 and TGFBR2
 TGFBR1 antibody  >5 publications with CD105 and TGFBR1
 GIPC1 antibody  >1 publications with CD105 and GIPC1
 TGFBI antibody  >1 publications with CD105 and TGFBI
 Products by Pathway  Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 TGF Beta Signaling Pathway antibodies  TGF Beta Signaling Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram
Diseases associated with anti-CD105 antibodyOrgans/Tissues associated with anti-CD105 antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >6400 publications with CD105 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >5580 publications with CD105 and Cardiovascular Diseases
 Heart Diseases Antibodies  >2915 publications with CD105 and Heart Diseases
 Breast Neoplasms Antibodies  >756 publications with CD105 and Breast Neoplasms
 Disease Models, Animal Antibodies  >742 publications with CD105 and Disease Models, Animal
 Inflammation Antibodies  >645 publications with CD105 and Inflammation
 Hypertension Antibodies  >612 publications with CD105 and Hypertension
 Liver Diseases Antibodies  >471 publications with CD105 and Liver Diseases
 Atherosclerosis Antibodies  >442 publications with CD105 and Atherosclerosis
 Necrosis Antibodies  >411 publications with CD105 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11185 publications with CD105 and Blood
 Bone Antibodies  >7978 publications with CD105 and Bone
 Brain Antibodies  >7856 publications with CD105 and Brain
 Heart Antibodies  >7821 publications with CD105 and Heart
 Muscle Antibodies  >6930 publications with CD105 and Muscle
 Vascular Antibodies  >6822 publications with CD105 and Vascular
 Nerve Antibodies  >2925 publications with CD105 and Nerve
 Skin Antibodies  >2865 publications with CD105 and Skin
 Connective Tissue Antibodies  >2410 publications with CD105 and Connective Tissue
 Eye Antibodies  >2028 publications with CD105 and Eye
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