View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Rat anti-Mouse CD117/c-Kit Monoclonal Antibody Catalog # MBS673626 Unit / Price 0.5 mg / $315 +1 FREE 8GB USB 5x0.5 mg / $1,250 +1 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name CD117/c-Kit, Monoclonal Antibody Full Product Name Rat Anti-Mouse CD117/c-kit-UNLB Product Synonym Names Rat Anti-Mouse CD117/c-kit - Purified, Unlabeled Antibody; Rat Anti-Mouse CD117/c-Kit Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype Rat IgG2b kappa Clone Number 2B8 Host Rat Species Reactivity Mouse Specificity CD117/c-KitThe CD117/c-kit antigen is the cell-surface receptor for Stem Cell Factor (SCF). Together these molecules constitute a ligand/receptor pair which functions to maintain normal hematopoiesis in the adult.1-3 Signaling through SCF/c-kit has an important role in stimulating myeloid and erythroid production of primitive hematopoietic progenitor cells.2 A variety of cytokines act synergistically with SCF to stimulate proliferation and differentiation of bone marrow progenitor cells. For example, SCF plus IL-7 can stimulate the combined myeloid and B-cell differentiation of uncommitted hematopoietic progenitor cells.2 SCF/c-kit also participates in erythropoiesis in both the bone marrow and spleen.1 Loss-of-function mutations for SCF/c-kit lead to a variety of pleiotropic developmental defects, while gain-of-function mutations can lead to constitutive activation of the kit receptor and links to cancer.3 Examples of the former are mast cell deficiency and severe macrocytic anemia, while the latter mutation has been identified in mastocytomas.3 Anti-CD117 mAbs have been used to block the function of the c-kit receptor in a variety of studies. These include the blockade of hematopoiesis1, 4 and disruption of melanocyte development. Form/Format Purified (UNLB) Antibody Characterization To ensure lot-to-lot consistency, each batch of product is tested for conformance to characteristics of a standard reference reagent by flow cytometry. Preparation and Storage The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Spectral Red (SPRD), Allophycocyanin-Cyanine 5.5 (APC-CY 5.5) and Allophycocyanin- Cyanine 7 (APC-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed. Other Notes Small volumes of anti-CD117/c-Kit antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Applications Tested/Suitable for anti-CD117/c-Kit antibody Flow Cytometry Application Notes for anti-CD117/c-Kit antibody Research Application(s): Cell homing studiesHematopoiesis and development studies TOP Product References and Citations for anti-CD117/c-Kit antibody 1. Broudy, V.C., et al, 1996. Blood 88:75.2. Veiby, O.P., S. D. Lyman and S. E. Jacobsen. 1996. Blood 87:3117.3. Sudo, T., et al. 1995. Oncogene 11:2469.4. Yoshida, H., et al. 1996. Development 122: 1207. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP