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anti-CD14 antibody :: Mouse CD14 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS213102
Unit / Price
0.1 mg  /  $230 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD14, Monoclonal Antibody

 Also Known As   

MOUSE ANTI HUMAN CD14

 Product Gene Name   

anti-CD14 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    158120
 3D Structure    ModBase 3D Structure for P08571
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 Clonality    Monoclonal
 Isotype    IgG2a
 Clone Number    UCHM1
 Host    Mouse
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 Form/Format    Purified
Purified IgG - liquid
 Concentration    IgG concentration 1.0 mg/ml (lot specific)
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 Perservative Stabilisers    0.09% Sodium Azide
Preparation
 Immunogen    Human Thymocytes followed by peripheral blood mononuclear cells
Histology Positive Control Tissue
 Target Species    Human
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-CD14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CD14 antibody

   Mouse anti Human CD14 antibody, clone UCHM1 recognizes a cell surface antigen of 55 kD, known as CD14.The CD14 molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology the CD14 molecule is found to be present on Langerhans cells, follicular dendritic cells, histiocytes and high endothelial venules. Antibodies to the CD14 molecule are known to induce oxidative burst formation. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative  (Hogg et al. 1984). UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.
 Applications Tested/Suitable for anti-CD14 antibody   

Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

 Application Notes for anti-CD14 antibody    Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
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 Testing Data of anti-CD14 antibody    Staining of human peripheral blood monocytes with Mouse anti Human CD14-IgG:Endotoxin Low (MBS224504)
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 Testing Data of anti-CD14 antibody    Staining of human peripheral blood monocytes with Mouse anti Human CD14:FITC (MBS211063)
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 Testing Data of anti-CD14 antibody    Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 (MBS212551) as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone UCHM1 as a detection reagent with purified CD14 as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively
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 Testing Data of anti-CD14 antibody    Staining of human peripheral blood monocytes with Mouse anti Human CD14:Biotin (MBS210506)
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 Testing Data of anti-CD14 antibody    Staining of human peripheral blood monocytes with Mouse anti Human CD14:Alexa Fluor 647
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 Testing Data of anti-CD14 antibody    Staining of human peripheral blood monocytes with Mouse anti Human CD14:APC (MBS210026)
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 Testing Data of anti-CD14 antibody    Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE (MBS213858)
anti-CD14 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for CD14. It may not necessarily be applicable to this product.
 NCBI GI #    4557417
 NCBI GeneID    929
 NCBI Accession #    NP_000582.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000591.3 [Other Products]
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 UniProt Primary Accession #    P08571 [Other Products]
 UniProt Secondary Accession #    Q53XT5; Q96FR6; Q96L99; Q9UNS3 [Other Products]
 UniProt Related Accession #    P08571 [Other Products]
 Molecular Weight    40,076 Da
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 NCBI Official Full Name    monocyte differentiation antigen CD14
 NCBI Official Synonym Full Names    CD14 molecule
 NCBI Official Symbol    CD14 [Similar Products]
 NCBI Protein Information    monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
 UniProt Protein Name    Monocyte differentiation antigen CD14
 UniProt Synonym Protein Names   
Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14Cleaved into the following 2 chains:Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form
 Protein Family    Monocyte differentiation antigen
 UniProt Gene Name    CD14 [Similar Products]
 UniProt Entry Name    CD14_HUMAN
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 NCBI Summary for CD14    The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]
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 UniProt Comments for CD14    CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; cell surface; extracellular region; plasma membrane; endosome membrane; lipid raft

Molecular Function: peptidoglycan receptor activity; protein binding; opsonin receptor activity; lipopolysaccharide binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; apoptosis; MyD88-independent toll-like receptor signaling pathway; positive regulation of endocytosis; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; phagocytosis; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to magnesium ion; response to ethanol; cell surface receptor linked signal transduction; response to heat; toll-like receptor signaling pathway; innate immune response; inflammatory response; response to electrical stimulus; toll-like receptor 4 signaling pathway; positive regulation of cytokine secretion
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 Research Articles on CD14    1. CD14 -260C/T polymorphism may increase the risk of gastric cancer in H. pylori-infected individuals. (Meta-analysis)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CD14 antibodyPathways associated with anti-CD14 antibody
 Reference Product  PubMed Publications
 TLR4 antibody  >1380 publications with CD14 and TLR4
 TLR2 antibody  >727 publications with CD14 and TLR2
 LBP antibody  >430 publications with CD14 and LBP
 ITGB2 antibody  >317 publications with CD14 and ITGB2
 LY96 antibody  >147 publications with CD14 and LY96
 TLR1 antibody  >101 publications with CD14 and TLR1
 TLR6 antibody  >62 publications with CD14 and TLR6
 LY86 antibody  >9 publications with CD14 and LY86
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway antibodies  Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 IKK Complex Recruitment Mediated By RIP1 Pathway antibodies  IKK Complex Recruitment Mediated By RIP1 Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
Diseases associated with anti-CD14 antibodyOrgans/Tissues associated with anti-CD14 antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2319 publications with CD14 and Inflammation
 Necrosis Antibodies  >2310 publications with CD14 and Necrosis
 Cardiovascular Diseases Antibodies  >647 publications with CD14 and Cardiovascular Diseases
 Lung Diseases Antibodies  >531 publications with CD14 and Lung Diseases
 Liver Diseases Antibodies  >360 publications with CD14 and Liver Diseases
 Nervous System Diseases Antibodies  >359 publications with CD14 and Nervous System Diseases
 Disease Models, Animal Antibodies  >330 publications with CD14 and Disease Models, Animal
 Atherosclerosis Antibodies  >311 publications with CD14 and Atherosclerosis
 Heart Diseases Antibodies  >280 publications with CD14 and Heart Diseases
 Asthma Antibodies  >259 publications with CD14 and Asthma
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7524 publications with CD14 and Blood
 Bone Antibodies  >1329 publications with CD14 and Bone
 Vascular Antibodies  >827 publications with CD14 and Vascular
 Liver Antibodies  >622 publications with CD14 and Liver
 Lung Antibodies  >597 publications with CD14 and Lung
 Kidney Antibodies  >334 publications with CD14 and Kidney
 Brain Antibodies  >307 publications with CD14 and Brain
 Heart Antibodies  >298 publications with CD14 and Heart
 Muscle Antibodies  >281 publications with CD14 and Muscle
 Intestine Antibodies  >238 publications with CD14 and Intestine
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