Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow CD146 rightarrow Monoclonal CD146  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Sialic acid (SA) ELISA Kit
 • hairy-enhancer-of-split related with YRPW motif 2 (HEY2) Antibody
 • microtubule-associated protein 1A (MAP1A) ELISA Kit
 • Lassa Virus IgM (LV-IgM) ELISA Kit
 • Regenerating Islet Derived Protein 3 Gamma (REG3g) ELISA Kit
 • Tumor Susceptibility Gene 101 (TSG101) ELISA Kit
 • VEGF Antibody
 • free thyroxine (FT4) ELISA Kit
 • T-complex Protein 1 epsilon (CCT5) Antibody
 • TLN1 - Talin Antibody
 • Androstenedione (ASD) Antibody
 • H-2 class II histocompatibility antigen, A-S beta chain (H2-... Recombinant Protein
 • TRIM38 Antibody
 • AADACL3 siRNA
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-CD146 antibody :: Mouse CD146 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS215541
Unit / Price
0.025 mg  /  $175 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD146, Monoclonal Antibody

 Also Known As   

MOUSE ANTI HUMAN CD146:FITC

 Product Gene Name   

anti-CD146 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    155735
 3D Structure    ModBase 3D Structure for P43121
Section Bar
 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    OJ79c
 Host    Mouse
Section Bar
 Form/Format    FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
 Concentration    IgG concentration 0.1 mg/ml (lot specific)
Section Bar
 Perservative Stabilisers    0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
 Buffer Solution    Phosphate buffered saline
 Target Species    Human
Section Bar
 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-CD146 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Product Description specifically for anti-CD146 antibody

   Mouse anti Human CD146 antibody, clone OJ79c recognizes human Cell surface glycoprotein MUC18, also known as CD146, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule or S-endo 1 endothelial-associated antigen. CD146 is a 646 amino acid single pass type 1 transmembrane glycoprotein with a calculated molecular mass of ~72 kDa. However due to extensive N-linked glycosylation CD146 migrates in polyacrylamide gels with an apparent molecular mass of ~:118 kDa. CD146 is a member of the immunoglobulin superfamily bearing 2 V-type Ig-like and 3 C-type Ig-like domains. CD146 is expressed by all endothelial cells and by melanoma cells and appears to act as an adhesion molecule (UniProt: P43121). Expression in melanoma may be linked to tumor progression (Lehmann et al. 1989). Mouse anti Human CD146 antibody, clone OJ79c is highly expressed on pericytes and has been utilized for the identification of perivascular mesenchymal precursor cells from cardiac muscle using flow cytometry (Chen et al. 2014).
 Applications Tested/Suitable for anti-CD146 antibody   

Flow cytometry (FC/FACS)

 Application Notes for anti-CD146 antibody    Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
Section Bar
 Testing Data of anti-CD146 antibody    Published customer image; Conjugated and unconjugated anti-CD326, anti-CD146 and anti-CD104 were analysed by flow cytometry for binding to CORL23 and LoVo cells [black line, negative control; blue line, unconjugated antibody; red line, immunoconjugates with porphyrin 1 (a) or porphyrin 2 (b)].From: Smith K, Malatesti N, Cauchon N, Hunting D, Lecomte R, van Lier JE, Greenman J, Boyle RW. Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action. Immunology. 2011 Feb;132(2):256-65.
anti-CD146 antibody Testing Data image
Section Bar
 Testing Data of anti-CD146 antibody    Published customer image: Brain-derived cell lines express markers for mesenchymal stem cells and pericytes but not for glial or neuronal precursors. (A) Representative histogram of flow cytometry analysis of cortical line and (B) ventricular zone line. Progenitors from all donors and both regions highly express MSC (CD90, CD73, CD105, CD29, CD166 and CD49d) and pericyte markers (CD140b/PDGFR-beta, RGS5, CD146, Nestin, a-SMA and NG2). They do not express hematopoietic (CD45), endothelial (CD31, CD34), microglial (CD14, CD11), glial or neuronal precursor cell markers (GFAP, O4) or myofibroblast markers (CD56), (green = isotype, red = respective marker).From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, et al. (2012) The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells. PLoS ONE 7(4): e35577.
anti-CD146 antibody Testing Data image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for CD146. It may not necessarily be applicable to this product.
 NCBI GI #    71274107
 NCBI GeneID    4162
 NCBI Accession #    NP_006491.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_006500.2 [Other Products]
Section Bar
 UniProt Primary Accession #    P43121 [Other Products]
 UniProt Secondary Accession #    O95812; Q59E86; Q6PHR3; Q6ZTR2 [Other Products]
 UniProt Related Accession #    P43121 [Other Products]
 Molecular Weight    57,605 Da
Table BarTOPTable Bar
 NCBI Official Full Name    cell surface glycoprotein MUC18
 NCBI Official Synonym Full Names    melanoma cell adhesion molecule
 NCBI Official Symbol    MCAM [Similar Products]
 NCBI Official Synonym Symbols   
CD146; MUC18
[Similar Products]
 NCBI Protein Information    cell surface glycoprotein MUC18; Gicerin; S-endo 1 endothelial-associated antigen; cell surface glycoprotein P1H12; melanoma adhesion molecule; melanoma-associated antigen A32; melanoma-associated antigen MUC18
 UniProt Protein Name    Cell surface glycoprotein MUC18
 UniProt Synonym Protein Names   
Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD_antigen: CD146
 UniProt Gene Name    MCAM [Similar Products]
 UniProt Synonym Gene Names    MUC18 [Similar Products]
 UniProt Entry Name    MUC18_HUMAN
Section Bar
 UniProt Comments for CD146    MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: extracellular space; focal adhesion; plasma membrane; integral to membrane; nucleus; external side of plasma membrane

Biological Process: anatomical structure morphogenesis; glomerular filtration; angiogenesis; cell adhesion; positive regulation of cell migration
Table BarTOPTable Bar
 Research Articles on CD146    1. With the ability of migration and survival in the advanced osteoarthritis cartilage, CD146+ chondroprogenitors might be "tissue-specific" for cartilage tissue regeneration.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-CD146 antibodyDiseases associated with anti-CD146 antibody
 Reference Product  PubMed Publications
 CD34 antibody  >23 publications with CD146 and CD34
 PROM1 antibody  >2 publications with CD146 and PROM1
 FYN antibody  >2 publications with CD146 and FYN
 SELE antibody  >1 publications with CD146 and SELE
 EDN1 antibody  >1 publications with CD146 and EDN1
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >34 publications with CD146 and Inflammation
 Lung Diseases Antibodies  >29 publications with CD146 and Lung Diseases
 Neoplasms, Experimental Antibodies  >24 publications with CD146 and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >23 publications with CD146 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >22 publications with CD146 and Disease Models, Animal
 Adenocarcinoma Antibodies  >22 publications with CD146 and Adenocarcinoma
 Lung Neoplasms Antibodies  >20 publications with CD146 and Lung Neoplasms
 Breast Neoplasms Antibodies  >19 publications with CD146 and Breast Neoplasms
 Prostatic Neoplasms Antibodies  >16 publications with CD146 and Prostatic Neoplasms
 Nervous System Diseases Antibodies  >16 publications with CD146 and Nervous System Diseases
Organs/Tissues associated with anti-CD146 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >125 publications with CD146 and Blood
 Vascular Antibodies  >101 publications with CD146 and Vascular
 Bone Antibodies  >49 publications with CD146 and Bone
 Skin Antibodies  >44 publications with CD146 and Skin
 Lung Antibodies  >43 publications with CD146 and Lung
 Embryonic Tissue Antibodies  >36 publications with CD146 and Embryonic Tissue
 Bone Marrow Antibodies  >34 publications with CD146 and Bone Marrow
 Muscle Antibodies  >30 publications with CD146 and Muscle
 Prostate Antibodies  >19 publications with CD146 and Prostate
 Brain Antibodies  >18 publications with CD146 and Brain
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions